Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Quantitative Analysis Comparison of Korea and China Scrophulariae Radix

국산과 중국산 현삼의 성분함량

  • Lee, Hye-Won (Department of Quality Control of Herbal Medicine, Korea Institute of Oriental Medicine) ;
  • Park, So-Young (Department of Quality Control of Herbal Medicine, Korea Institute of Oriental Medicine) ;
  • Choo, Byung-Kil (Department of Quality Control of Herbal Medicine, Korea Institute of Oriental Medicine) ;
  • Chun, Jin-Mi (Department of Quality Control of Herbal Medicine, Korea Institute of Oriental Medicine) ;
  • Lee, A-Yeong (Department of Quality Control of Herbal Medicine, Korea Institute of Oriental Medicine) ;
  • Kim, Ho-Kyoung (Department of Quality Control of Herbal Medicine, Korea Institute of Oriental Medicine)
  • 이혜원 (한국한의학연구원 검사사업부) ;
  • 박소영 (한국한의학연구원 검사사업부) ;
  • 추병길 (한국한의학연구원 검사사업부) ;
  • 천진미 (한국한의학연구원 검사사업부) ;
  • 이아영 (한국한의학연구원 검사사업부) ;
  • 김호경 (한국한의학연구원 검사사업부)
  • Published : 2007.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Scrophulariae Radix(Scrophulariaceae) is a perennial growing in all parts of korea, China and Asia. It has been used as a folk medicine in treating guttural diseases acting as an antiphlogistics, antipyretics and analgesics. In this report, to compare with Korea and China product, quantitative analysis of harpagoside and p-methoxy cinnamic acid(4-MCA) was performed by HPLC method. Using a Luna $C_{18}(2)$ column, we carried out quantitative analysis with 0.1% phosphoric acid in water and acetonitrile as gradient condition. Harpagoside and 4-MCA were detected at retention time of about 11.5 and 15.0 min, respectively. Contents of harpagoside and 4-MCA proved that Korean products were higher than China more than twice.

Keywords

References

  1. 전국한의과대학본초학교수 공편저 (2000) 본초학, 192-193. 영림사, 서울
  2. 강병수, 서부일, 최호영 (2003) 한약 포제와 임상응용, 244-245. 영림사, 서울
  3. Kim, S. R., Kang, S. Y., Lee, K. Y., Kim, S. H., Markelonis, G. J., Oh, T. H. and Kim, Y. C. (2003) Anti-amnestic activity of E-p-methoxycinnamic acid from Scrophularia buergeriana. Cog. Bra. Res. 17: 454-461 https://doi.org/10.1016/S0926-6410(03)00161-7
  4. Lee, E. J., Kim, S. R., Kim, J. and Kim, Y. C. (2002) Hepatoprotective phenylpropanoids from Scrophularia buergeriana roots against CCl(4)-induced toxicity: action mechanism and structure-activity relationship. Planta Med. 68(5): 407-411 https://doi.org/10.1055/s-2002-32081
  5. 신국현, 이상현, 지형준 (1999) 고속액체크로마토그라피에 의한 현삼근 중 p-Methoxycinnamic acid의 정량. 생약학회지 30(3): 328-331
  6. 한방약리학 교재편찬위원회 (2005) 한방약리학, 693-695. 신일상사, 서울
  7. 육창수, 심재호, 류기타, 김정근 (2000) 한약학 II <기원, 약리, 처방, 임상응용> 147-149. 광명의학사, 서울
  8. 우원식 (1963) 현삼의 유효성분, p-methoxycinnamic acid에 관한 연구. p-methoxycinnamic acid의 동정 및 그 해열작용. 약학회지 7: 55-57
  9. 우원식 (1965) p-methoxycinnamic acid의 진통작용. 약학회지 9: 31-33
  10. Sirichai, A., Sophon, R., Walter, H. H. and Sirinton, Y. (2005) Mechanisms of antihyperglycemic effect of p-methoxycinnamic acid in normal and streptozotocin-induced diabetic rats. Life Sci. 78: 406-421 https://doi.org/10.1016/j.lfs.2005.04.073
  11. Tom, H-W. H., Van, H. T., Rujee, K. D., Sharon. T., Sigrun, C., Basil D. R. and Colin, C. D. (2006) Harpagoside suppresses lipopolysacchride-induced iNOS and COX-2 expression through inhibition of NF-kB activation. J. of Ethno. 104: 149-155 https://doi.org/10.1016/j.jep.2005.08.055
  12. 국가약전위원회 (2005) 중화인민공화국약전, 76-77. 화학 공업출판사, 북경
  13. 식품의약품안전청 (2002) 대한약전 제8개정, 1654-6. 약업 신문, 서울
  14. 이준영, 이은일 (2001) 보건의학통계학, 43-58. 계축문화사, 서울