Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Apoptotic Cell Death by Methanol Extract of Phellinus linteus-Barley Corn in Human Leukemic U937 Cells through Induction of p21 and Bax, and Activation of Caspase-3

상황보리 추출물에 의한 p21 및 Bax 발현 증가와 caspase 활성화를 통한 U937 인체백혈병 세포의 apoptosis 유발

  • Park, Cheol (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Kim, Hyun-Joog (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Chung, Kyung-Tae (Department of Biomedical Laboratory Science, College of Natural Science, Dong-Eui University) ;
  • Yoon, Tae-Kyung (Environmental Engineering Major, College of Engineering, Dong-Eui University) ;
  • Choi, Byung-Tae (Department of Anatomy, Pusan National University Graduate School of Oriental Medicine) ;
  • Lee, Yong-Tae (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Park, Dong-Il (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Choi, Yung-Hyun (Department of Oriental Medicine, College of Oriental Medicine, Dong-Eui University)
  • 박철 (동의대학교 한의과대학 한의학과) ;
  • 김현중 (동의대학교 한의과대학 한의학과) ;
  • 정경태 (동의대학교 자연과학대학 임상병리학과) ;
  • 윤태경 (동의대학교 공과대학 환경공학과) ;
  • 최병태 (부산대학교 한의학전문대학원 해부학교실) ;
  • 이용태 (동의대학교 한의과대학 한의학과) ;
  • 박동일 (동의대학교 한의과대학 한의학과) ;
  • 최영현 (동의대학교 한의과대학 한의학과)
  • Published : 2007.10.25
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Abstract

Phellinus linteus is a well-known Oriental medicinal fungus that has various biological activities, including immunomodulatory and anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the anti-proliferative activity of the methanol extract of P. linteus-Barley corn (MEPLB) in human lekemic U937 cells. It was found that exposure of U937 cells to MEPLB resulted morphological change and growth inhibition in a dose-dependent manner as measured by trypan blue count and MTT assay. Upon treatment with MEPLB, U937 cells developed many of the hallmark features of apoptosis, including condensation of chromatin and an increase in the sub-G1 population suggesting that the anti-proliferative effect of MEPLB is associated with the induction of apoptosis. The anti-proliferative and apoptotic effects of MEPLB were connected with a marked induction of the pro-apoptotic Bax and cyclin-dependent kinase (Cdk) inhibitor p21 in a p53-independent manner. Additionally, MEPLB treatment significantly induced the caspase-3 activity in U937 cells. Taken together, the present results suggest that apoptotic signals evoked by MEPLB in human leukemic U937 cells may converge caspase-3 activation through an up-regulation of Bax rather than a down-regulation of Bcl-2 or Bel-xL.

Keywords

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