Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Tyrosinase Inhibitory Xanthones from Cudrania tricuspidata

꾸지뽕나무에서 분리한 xanthones의 tyrosinase 저해제 연구

  • Oh, Sang-Seok (Department of Horticulture, Gyeongsang National University) ;
  • Seo, Eun-Jin (Division of Applied Life Science(BK2l Program), Environmental Biotechnology National Core Research Center, Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Hoi-Young (Division of Applied Life Science(BK2l Program), Environmental Biotechnology National Core Research Center, Research Institute of Life Science, Gyeongsang National University) ;
  • Ryu, Young-Bae (Division of Applied Life Science(BK2l Program), Environmental Biotechnology National Core Research Center, Research Institute of Life Science, Gyeongsang National University) ;
  • Lee, Jin-Hwan (Division of Applied Life Science(BK2l Program), Environmental Biotechnology National Core Research Center, Research Institute of Life Science, Gyeongsang National University) ;
  • Gal, Sang-Wan (Department of Microbiological Engineering, Jinju National University) ;
  • Park, Ki-Hun (Division of Applied Life Science(BK2l Program), Environmental Biotechnology National Core Research Center, Research Institute of Life Science, Gyeongsang National University)
  • 오상석 (경상대학교 원예학과) ;
  • 서은진 (경상대학교 응용생명과학부) ;
  • 김회영 (경상대학교 응용생명과학부) ;
  • 류영배 (경상대학교 응용생명과학부) ;
  • 이진환 (경상대학교 응용생명과학부) ;
  • 갈상완 (진주산업대학교 미생물공학과) ;
  • 박기훈 (경상대학교 응용생명과학부)
  • Published : 2007.04.25
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Abstract

The methanolic roots bark extract of Cudrania tricuspidata (Carr.) Bureau was chromatographed, which yielded three xanthones 1-3 by tyrosinase inhibitory activity-guided fractionation. The structures were fully characterized by analysis of physical and spectral data. Among them, furano prenylxanthone 3, never reported as tyrosinase inhibitor, showed potent activity with $IC_{50}$ value of $16.5{\mu}M$, and appeared to inhibit the polyphenol oxidase activity of tyrosinase in an uncompetitive inhibitor($K_i=1.6{\mu}m$) when L-tyrosine was used as a substrate. Moreover, potent inhibitor furano prenylxanthone 3 had an extended lag time of 310 sec at $20{\mu}M$, while lag time of kojic acid as positive control was prolonged with 350 sec at the same concentration.

꾸지뽕나무(Cudrania tricuspidata(Carr.) Bureau) 뿌리껍질에서 3종의 xanthones 화합물을 분리하였으며, 분광학적인 자료를 바탕으로 cudraxanthone L (1), cudraxanthone D (2), 그리고 cudraxanthone M (3)으로 구조동정되었다. 분리된 화합물을 L-tyrosine이 기질로 작용할 때 tyrosinase 저해 활성을 측정한 결과 cudraxanthone M (3)의 $IC_{50}$ 값이 $16.5{\mu}M$로 가장 높은 저해 효능을 보였으며, kinetic type은 uncompetitive inhibition로 저해 상수($K_i$)는 $1.6{\mu}M$로 측정되었다. 또한 이 화합물은 $20{\mu}M$의 농도에서 lag time이 310초로 측정 되어 대조화합물인 kojic acid와 유사한 저해능을 나타내었다.

Keywords

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