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Study of Genetic Diversity and Taxonomy of Genus Sorbus in Korea Using Random Amplified Polymorphic DNA

RAPD에 의한 한국내 마가목속 식물의 유전적 다양성과 분류학적 연구

  • Park, So-Hye (Department of Molecular Biology, Dongeui University) ;
  • Kim, Sea-Hyun (Biotechnology Division of Forest Genetics, Korea Forest Research Institute) ;
  • Seo, Hee-Won (Biotechnology Division of Forest Genetics, Korea Forest Research Institute) ;
  • Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
  • 박소혜 (동의대학교 분자생물학과) ;
  • 김세현 (임업연구원 산림유전자원부) ;
  • 서희원 (임업연구원 산림유전자원부) ;
  • 허만규 (동의대학교 분자생물학과)
  • Published : 2007.04.25

Abstract

Genus Sorbus is a long lived woody species. Plants of this genus are primarily distributed patchy throughout Asia and Europe. Sorbus commixta is primarily distributed throughout Europe. Eastern Asian Sorbus species are regarded as very important herbal medicines in Korea and China. Random amplified polymorphic DNA (RAPD) was used to investigate the genetic variation and phylogenetic analysis of four species of this genus in Korea. Although some Korean populations of these species were isolated and patchily distributed, they exhibited a high level of genetic diversity. Twenty-six primers revealed 205 loci, of which 128 were polymorphic (62.4%). S. commixta showed the highest diversity (0.165), whereas S. aucuparia showed the lowest diversity (0.109). The estimated gene flow (Nm) was low high among intra-species (mean Nm=0.755). A similarity matrix based on the proportion of shared fragments (GS) was used to evaluate relatedness among species. The estimate of GS ranged from 0.786 to 0.963. The molecular data allowed us to resolve well-supported clades in Korean taxa and European species. An addition, especially, species-specific markers for genus Sorbus by RAPD analysis may be useful in germ-plasm classification and agricultural process of several taxa of this genus.

마가목 속(genus Serbus)은 아시아와 유럽에 분포되어 있는 목본이다. 유럽마가목(Sorbus commixta)은 유럽에 일차적으로 분포한다. 마가목 속 식물은 한국과 중국에서 약용으로 쓰인다. 한국내 마가목 속 식물에 대해 RAPD에 의한 유전적 변이와 계통관계를 조사하였다. 한국 내 마가목 속 식물은 비록 패치분포를 보이나 높은 유전적 다양도를 가지고 있었다. 26 시발체로 205 유전자좌위를 얻었으며 그 중 128 개(62.4%)는 다형성을 나타내었다. 마가목(S. commixta)이 가장 높은 유전적 다양도를 나타낸 반면(0.165), 유럽마가목(S. aucuparia)이 가장 낮은 유전적 다양도를 나타내었다(0.109). 종간 세대를 통해 이주하는 유전자수(Nm)는 매우 낮았다(평균 Nm=0.755). 분류군간 유사도는 0.786에서 0.963사이에 있었다. 이 분자마크(RAPD)로 한국내 분류군과 유럽종간 구분이 잘 되었다. 또한 종 특이 마크로 종 동정에 이용할 수 있었으며, 종의 보전이나 생식질 보전에 기초로 이용될 수 있을 것으로 사료된다.

Keywords

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