Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Susceptibility of the Alfalfa Weevil, Hypera postica (Coleoptera: Curculionidae) to Korean Entomopathogenic Nematodes in Laboratory Assays

한국산 곤충병원성 선충에 대한 알팔파바구미의 감수성 실내 검정

  • Kim, Hyeong-Hwan (Horticultural Environment Division, National Horticultural Research Institute) ;
  • Han, Gun-Yeong (Department of Applied Biology and Environmental Sciences, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Park, Chung-Chan (Department of Applied Biology and Environmental Sciences, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Choo, Ho-Yul (Department of Applied Biology and Environmental Sciences, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Cho, Sung-Rae (Gyeongnam Agricultural Research and Extensiion Services) ;
  • Lee, Heung-Soo (Gyeongnam Agricultural Research and Extensiion Services) ;
  • Lee, Dong-Woon (Department of Applied Biology, Sangju National University) ;
  • Park, Chung-Gyoo (Department of Applied Biology and Environmental Sciences, Institute of Agriculture and Life Sciences, Gyeongsang National University)
  • 김형환 (원예연구소 원예환경과) ;
  • 한건영 (경상대학교 응용생물환경학과 농업생명과학연구원) ;
  • 박정찬 (경상대학교 응용생물환경학과 농업생명과학연구원) ;
  • 추호렬 (경상대학교 응용생물환경학과 농업생명과학연구원) ;
  • 조성래 (경남농업기술원) ;
  • 이홍수 (경남농업기술원) ;
  • 이동운 (상주대학교 생물응용학과) ;
  • 박정규 (경상대학교 응용생물환경학과 농업생명과학연구원)
  • Published : 2007.04.30
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Abstract

Laboratory tests were conducted to determine the susceptibility of the alfalfa weevil, Hypera postica to Korean entomopathogenic nematodes, Steinernema carpocapsae GSN1 strain (ScG), S. glaseri Dongrae strain (SgD), Heterorhabditis bacteriophora Hamyang strain (HbH), and Heterorhabditis sp. Gyeongsan strain (HeG) at the petri dish assay. The larval mortality of H. postica was significantly different depending on nematode species and treatment concentration. SgD and HeG strain were more effective against H. postica larva than ScG and HbH strain. When SgD and HeG strain were treated with the rate of >20 infective juveniles (ijs) per alfalfa weevil larva, mortality was ca. 77.5-100% at the late instars of H. postica in 3 days. The number of established nematode was significantly different depending on nematode species, whereas number of progeny was not significantly different. The mean number of established us of SgD strain in a host was the highest at 80 ijs by 30.2. The highest progeny number of HeG strain was 2,671.5 with 80 ijs. All nematode strains were not parasitic entirely to H. postica adults. These results show that alfalfa weevil late larva is highly susceptible to Korean entomopathogenic nematodes in the laboratory.

한국산 곤충병원성 선충 S. carpocapsae GSN1 계통(ScG), S. glaseri Dongrae 계통(SgD), Heterorhabditis bacteriophora Hamyang 계통(HbH) 및 Heterorhabditis spp. Cyeongsan 계통(HeG)에 대한 알팔파바구미의 감수성을 실내 페트리디쉬 검정하였다. 알팔파바구미의 유충 치사율은 선충 종과 처리농도에 따라 유의적인 차이가 있었다. 알팔파바구니의 유충 한 마리당 각 선충 계통의 감염충(3령충)을 20마리 이상 접종했을 때 3일 후 77.5(HbH)-100(SgD)의 높은 치사율을 나타내었다. 선충의 침입수는 선충 종과 처리농도에 따라 차이가 있었으나, 증식수는 유의적 인 차이가 없었다. 침 입수에서는 SgD를 알팔파바구미 유충 한 마리당 80마리로 접종했을 때 30.2마리로 가장 많았다. 그리고 증식수는 HeG를 알팔파바구미 유충 한 마리당 80마리로 접종했을 때 2,671.5로 가장 많았다. 한국산 곤충병원성 선충 4 계통은 알팔파바구미의 성충에는 전혀 기생하지 않았으나 노숙 유충에 대해서는 매우 감수성이었다.

Keywords

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