Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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High-throughput Gene Expression Analysis to Investigate Host-pathogen Interaction in Avian Coccidiosis

  • Lillehoj Hyun, S. (Animal Parasitic Disease laboratory Animal and Natural Resources Institute Beltsville Agricultural Research Center United States Department of Agriculture Agricultural Research Service)
  • Published : 2007.03.31
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Abstract

Poultry products including meat and eggs constitute a major protein source in the American diet and disease-causing pathogens represent major challenges to the poultry industry. More than 95% of pathogens enter the host through the mucosal surfaces of the respiratory, digestive and reproductive tracts and over the past few decades, the two main mechanisms used to control diseases have been the use of vaccines and antibiotics. However, in the poultry industry, there are mounting concerns over the ability of current vaccines to adequately protect against emerging hyper-virulent strains of pathogens and a lack of suitable, cost effective adjuvants. Thorough investigation of the immunogenetic responses involved in host-pathogen interactions will lead to the development of new and effective strategies for improving poultry health, food safety and the economic viability of the US poultry industry. In this paper, I describe the development of immunogenomic and proteomic tools to fundamentally determine and characterize the immunological mechanisms of the avian host to economically significant mucosal pathogens such as Eimeria. Recent completion of poultry genome sequencing and the development of several tissue-specific cDNA libraries in chickens are facilitating the rapid application of functional immunogenomics in the poultry disease research. Furthermore, research involving functional genomics, immunology and bioinformatics is providing novel insights into the processes of disease and immunity to microbial pathogens at mucosal surfaces. In this presentation, a new strategy of global gene expression using avian macrophage (AMM) to characterize the multiple pathways related to the variable immune responses of the host to Eimeria is described. This functional immunogenomics approach will increase current understanding of how mucosal immunity to infectious agents operates, and how it may be enhanced to enable the rational development of new and effective strategies against coccidiosis and other mucosal pathogens.

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References

  1. Abbas AK, Murphy KM, Sher A 1996 Functional diversity of helper T lymphocytes. Nature 383:787-793 https://doi.org/10.1038/383787a0
  2. Ashkar S, Weber GF, Panoutsakopoulou V, Sanchirico ME, Jansson M, Zawaideh S, Rittling SR, Denhardt DT, Glimcher MJ, Cantor H 2000 Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity. Science 287: 860- 864 https://doi.org/10.1126/science.287.5454.860
  3. Bedard PA, Balk SD, Gunther HS, Morisi A, Erikson RL 1987 Repression of quiescence-specific polypeptides in chicken heart mesenchymal cells transformed by Rous sarcoma virus. Mol Cell Biol 7: 1450-1458 https://doi.org/10.1128/MCB.7.4.1450
  4. Bliss TW, Dohms JE, Emara MG, Keeler CL Jr 2005 Gene expression profiling of avian macrophage activation. Vet Immunol Immunopathol 105: 289-299 https://doi.org/10.1016/j.vetimm.2005.02.013
  5. Chapman HD 1998 Evaluation of the efficacy of anticoccidial drugs against Eimeria species in the fowl. Int J Parasitol 28: 1141-1144 https://doi.org/10.1016/S0020-7519(98)00024-1
  6. Cogburn LA, Wang X, Carre W, Rejto L, Aggrey SE, Duclos MJ, Simon J, Porter TE 2004 Functional genomics in chickens: Development of integrated-systems microarrays for transcriptional profiling and discovery of regulatory pathways. Comp Funct Genomics 5:253-261 https://doi.org/10.1002/cfg.402
  7. Dalloul R, Lillehoj H 2005 Recent advances in immunomodulation and vaccination strategies against coccidiosis. Avian Diseases 49:1-8 https://doi.org/10.1637/7306-11150R
  8. Dalloul RA, Bliss TW, Hong YH, Ben-Chouikha I, Park DW, Keeler CL, Lillehoj HS 2007 Unique responses of the avian macrophage to different species of Eimeria. Mol Immunol In Press
  9. Dalloul RA, Lillehoj HS 2006 Poultry coccidiosis: Recent advancements in control measures and vaccine development. Expert Rev Vaccines 5:143-163 https://doi.org/10.1586/14760584.5.1.143
  10. Gavora JS 1990 Disease genetics. In: Poultry Breeding and Genetics, ed. R. D. Crawford. Elsevier, New York
  11. Laurent F, Mancassola R, Lacroix S, Menezes R, Naciri M 2001 Analysis of chicken mucosal immune response to Eimeria tenella and Eimeria maxima infection by quantitative reverse transcription-PCR. Infect. Immun 69:2527-2534 https://doi.org/10.1128/IAI.69.4.2527-2534.2001
  12. Lillehoj HS, Choi KD 1998 Recombinant chicken interferon-gamma-mediated inhibition of Eimeria tenella development in vitro and reduction of oocyst production and body weight loss following Eimeria acervulina challenge infection. Avian Dis 42:307-314 https://doi.org/10.2307/1592481
  13. Lillehoj HS, Choi KD, Jenkins MC, Vakharia VN, Song KD, Han JY, Lillehoj EP 2000 A recombinant Eimeria protein inducing chicken interferon-${\gamma}$ production. Comparison of different gene expression systems and immunization strategies for vaccination against coccidiosis. Avian Disease 44:379- 389 https://doi.org/10.2307/1592553
  14. Lillehoj HS, Jenkins MC, Bacon LD, Fetterer RH, Briles WE 1988 Eimeria acervulina: Evaluation of the cellular and antibody responses to recombinant coccidial antigens in B-congenic chickens. Experimental Parasitology 67:148-158 https://doi.org/10.1016/0014-4894(88)90062-8
  15. Lillehoj HS, Min WG, Dalloul RA 2004 Recent progress on the cytokine regulation of intestinal immune responses to Eimeria. Poult Science. 83:611-623 https://doi.org/10.1093/ps/83.4.611
  16. Lillehoj HS, Yun CH, Lillehoj EP 1999 Recent progress in poultry vaccine development against coccidiosis. Korean J Poultry Sci 26:149-170
  17. Mao PL, Beauchemin M, Bedard PA 1993 Quiescence- dependent activation of the p20K promoter in growth- arrested chicken embryo fibroblasts. J Biol Chem 268: 8131- 8139
  18. Min Wongi, Lillehoj HS, Ashwell CM, van Tassell C, Matukumalli LK, Han JY, Lillehoj EP 2005 EST analysis of Eimeria-activated intestinal intra-epithelial lymphocytes in chickens. Molecular Biotechnology. 30:143-150 https://doi.org/10.1385/MB:30:2:143
  19. Min Wongi, Lillehoj HS, Kim Sungwon, Zhu JJ, Beard H, Alkharouf N, Matthews BF 2003 Profiling local gene expression changes associated with Eimeria maxima and Eimeria acervulina using cDNA microarray. Appl Microbiol Biotechnol 62:392-399 https://doi.org/10.1007/s00253-003-1303-x
  20. Morgan RW, Sofer L, Anderson AS, Bernberg EL, Cui J, Burnside J 2001 Induction of host gene expression following infection of chicken embryo fibroblasts with oncogenic Marek's disease virus. J Virol 75:533-539 https://doi.org/10.1128/JVI.75.1.533-539.2001
  21. Neiman PE, Grbic JJ, Polony TS, Kimmel R, Bowers SJ, Delrow J, Beemon KL 2003 Functional genomic analysis reveals distinct neoplastic phenotypes associated with c-myb mutation in the bursa of Fabricius. Oncogene 22:1073-1086 https://doi.org/10.1038/sj.onc.1206070
  22. O'Regan AW, Nau GJ, Chupp GL, Berman JS 2000 Osteopontin (Eta-1) in cell-mediated immunity: Teaching an old dog new tricks. Immunol Today 21: 475-478 https://doi.org/10.1016/S0167-5699(00)01715-1
  23. Patarca R, Saavedra R.A, Cantor H 1993 Molecular and cellular basis of genetic resistance to bacterial infection: the role of the early T-lymphocyte activation-1/osteopontin gene. Crit Rev Immunol 13: 225-246
  24. Rodenburg RJ, Brinkhuis RF, Peek R, Westphal JR, Van Den Hoogen FH, van Venrooij WJ, van de Putte LB 1998 Expression of macrophage-derived chemokine (MDC) mRNA in macrophages is enhanced by interleukin-1beta, tumor necrosis factor alpha, and lipopolysaccharide. J Leukoc Biol 63:606-611 https://doi.org/10.1002/jlb.63.5.606
  25. Song KD, Lillehoj HS, Choi KD, Parcells MS, Huynh JT, Yun CH and Han JY 2000 A DNA vaccine encoding a conserved Eimera protein induces protective immunity against live Eimeria acervulina challenge. Vaccine 19, 243-252 https://doi.org/10.1016/S0264-410X(00)00169-9
  26. Trout JM, Lillehoj HS 1996 T lymphocyte roles during Eimeria acervulina and Eimeria tenella infections. Vet Immunol Immunopathol 53:163-172 https://doi.org/10.1016/0165-2427(95)05544-4
  27. Yun CH, Lillehoj HS, Choi KD 2000 Eimeria tenella infection induces local gamma interferon production and intestinal lymphocyte subpopulation changes. Infect Immun 68:1282- 1288 https://doi.org/10.1128/IAI.68.3.1282-1288.2000
  28. Zhu J, Lillehoj HS, Allen PC, Min W, Van Tassell C, Sonstegard TS, Cheng HH, Pollock DL, Sadjadi M, Emara M 2003 Mapping quantitative trait loci associated with resistance to coccidiosis and growth. Poultry Science 82:9-16 https://doi.org/10.1093/ps/82.1.9
  29. Zhu J, Lillehoj HS, Allen PC, Yun CH, Pollock D, Emara MG 2000 Analysis of disease resistance associated parameters in broiler chickens challenged with Eimeria maxima. Poultry Sci 79:619-625 https://doi.org/10.1093/ps/79.5.619