Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Genotoxicity Study of Magnolia obovata Extracts

후박 추출물의 유전독성평가

  • Lee, Seung-Ho (PeerBiotech Co., Ltd.) ;
  • Ryu, Jae-Myun (PeerBiotech Co., Ltd.) ;
  • Seo, Im-Kwon (PeerBiotech Co., Ltd.) ;
  • Lee, Tae-Hee (PeerBiotech Co., Ltd.) ;
  • Kim, Yun-Bae (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Moon, Sung-Kwon (Department of Food & Biotechnology, Chungju National University) ;
  • Jung, Kyung-Hwan (Department of Food & Biotechnology, Chungju National University) ;
  • Park, Ki-Rang (Department of Biotechnology and Clinical Laboratory Science, Juseong College) ;
  • Hwang, Seock-Yeon (Department of Biotechnology and Clinical Laboratory Science, Juseong College)
  • Published : 2007.03.31
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Abstract

To evaluate the immuno-toxicity of magnolia extracts, mutagenicity of Salmonella, chromosome aberration of Chinese hamster ovary (CHO) cells and micronucleus formation in rats were examined. Magnolia extracts at the concentrations of $312{\sim}5,000{\mu}g/plate$ did not induce mutagenicity in Salmonella typhimurium TA 98, TA 100 and TA 1535 with and without metabolic activation of S-9 mixture. In chromosome aberration assay, Magnolia extracts at the concentrations of $50{\sim}800{\mu}g/plate$ did not cause a significant chromosome aberration in CHO cells with and without metabolic activation of S-9 mixture. Magnolia extracts were treated with dose of 0.5, 1 and 2 g/kg in ICR mice. After 48 hours, the frequencies of the micro-nucleided polychromasia erythrocytes (MNPCE) were determined in bone marrows isolated from the mice. Magnolia extracts did not increase the incidence of polychromasia erythrocytes of bone marrow in ICR mice. These results show that Mgnolia extracts did not induce any harmful genotoxic effects.

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References

  1. Ames, B.N., McCann, J. and Yamasaki, E. (1975). Methods for detecting carcinogens & mutagenes with the salmonella/ mammalian-microsome mutagenicity test. Mutat. Res., 31, 347-364 https://doi.org/10.1016/0165-1161(75)90046-1
  2. Bai, X., Cerimele, F., Ushio-Fukai, M., Waqas, M., Campbell,P.M., Govindarajan, B., Der, C.J., Battle, T., Frank, D.A., Ye, K., Murad, E., Dubiel, W., Soff, G. and Arbiser, J.L. (2003). Honokiol, a small molecular weight natural product, inhibits angiogenesis in vitro and tumor growth in vivo. J. Biol. Chem., 278, 35501-35507 https://doi.org/10.1074/jbc.M302967200
  3. Boca Raton, F.L. (2002). Li TSC. Chinese and Related North American Herbs: Phytopharmacology and Therapeutic Values. CRC Press
  4. Hayashi, M., Sofuni, T. and Ishidate, M. Jr. (1984). A pilot experiment for the micronucleus test: The multi-sampling at multi-dose levels method. Mutat. Res., 141, 165-169 https://doi.org/10.1016/0165-7992(84)90091-5
  5. Hayashi, M., Yoshimura, I., Sofuni, T. and Ishidate, M. Jr. (1989). A procedure for data analysis of the rodent micronucleus test involving a historical control. Environ. Mol. Mutagen., 13, 347-356 https://doi.org/10.1002/em.2850130412
  6. Kenji, I., Teru, H., Makoto, H., Noopur, R., Shaji, K., Hiromasa, H., Norihiko, S., Hiroshi, Y., Aldo, M.R., Paul, R., Klaus, P., Steven, L.G., Dharminder, C., Kazuo, T., Jack, A. and Kenneth, C.A. (2005). Honokiol overcomes conventional drug resistance in human multiple myeloma by induction of caspase-dependent and -independent apoptosis. Blood, 106, 1794-1800 https://doi.org/10.1182/blood-2005-01-0346
  7. Kim, B.S. and Margolin, B.H. (1999). Statistical methods for the Ames Salmonella assay: a review. Mutant. Res., 436, 113-122
  8. Maron, D.M. and Ames, B.N. (1983). Revised methods for the Salmonella mutagenicity test. Mutat. Res., 113, 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  9. Yang, S.E., Hsieh, M.T., Tsai, T.H. and Hsu, S.L. (2003). Effector mechanism of magnolol-induced apoptosis in human lung squamous carcinoma CH27 cells. Br. J. Pharmacol., 138, 193201 https://doi.org/10.1038/sj.bjp.0705024
  10. 식품의약품안전청고시 제1999-61호 (1999). 의약품 등의 독성시험기준-유전독성시험