전기학회논문지 (The Transactions of The Korean Institute of Electrical Engineers)

  • 제56권3호
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  • Pages.561-565
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  • 2007
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  • 1975-8359(pISSN)
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  • 2287-4364(eISSN)
대한전기학회 (The Korean Institute of Electrical Engineers)
권호 표지

연속흐름 중합효소연쇄반응칩 제작을 위한 인듐 산화막 전극의 특성분석

Characteristics of Indium-Tin-Oxide Electrode for Continuous-flow PCR Chip

  • 발행 : 2007.03.01
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⟨ 이전 논문 다음 논문 ⟩

초록

We propose glass and PDMS (polydimethylsiloxane) chips for DNA amplification with continuous-flow PCR (polymerase chain reaction). The PDMS microchannel was fabricated using a negative molding method for sample injection. Three heaters and sensors of ITO (indium-tin-oxide) thin films were fabricated on glass chip. ITO heaters and sensors were calibrated accurately for the temperature control of the liquid flow. ITO heater generated stable heat versus applied power. ITO sensor resistance was changed linearly versus temperature increase as a RTD (resistance temperature detector) sensor. As a result, we enable precision temperature control of continuous-flow PCR chip. Using the continuous-flow PCR chip DNA plasmid pKS-GFP 720 bp was successfully amplified.

키워드

참고문헌

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