Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Efficient Gene Delivery through the Human Transferrin Receptor of Fibroblast-like Synoviocytes Stimulated with bFGF: a Potential Target Receptor for Gene Transduction in Rheumatoid Arthritis

  • Kim, Hak-Jae (Kohwang Medical Research Institute, Kyung Hee University) ;
  • Joung, In-Sil (Department of Biological Science, Hanseo University) ;
  • Nah, Seong-Su (Kohwang Medical Research Institute, Kyung Hee University) ;
  • Lee, Kyu-Hoon (Department of Physical Medicine and Rehabilitation, College of Medicine, Hanyang University) ;
  • KimKwon, Yun-Hee (Department of Biology and Department of Life and Nanopharmaceutical Science, Kyung Hee University) ;
  • Chung, Joo-Ho (Kohwang Medical Research Institute, Kyung Hee University) ;
  • Hong, Seung-Jae (Kohwang Medical Research Institute, Kyung Hee University)
  • Published : 2007.06.30
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Abstract

Efficient gene delivery to specific tissues, such as inflammatory and cancerous tissues, is currently a major concern in disease treatment. The human transferrin receptor (hTR) has been detected in the synovium and fibroblast-like synoviocytes (FLS), which raises the possibility that expression of hTR is associated with the pathogenesis of rheumatoid arthritis (RA). To investigate whether the hTR is a useful target for gene transduction into the FLS of RA patients, recombinant adenoviruses with wildtype fiber (AdLac) and transferrin peptide-tagged fiber (Tf-AdLac) were used. The hTR expression level in FLS was notably increased by basic fibroblast growth factor (bFGF). Gene transduction to FLS was significantly higher by the hTR-targeted adenovirus than by the AdLac adenovirus, and treatment of the FLS with bFGF resulted in increased gene transduction by Tf-AdLac. Taken together, these data support Tf-AdLac as a new strategy for gene transduction in the treatment of RA patients.

Keywords

References

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