한국어류학회지 (Korean Journal of Ichthyology)

  • 제19권2호
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  • Pages.88-92
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  • 2007
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  • 1225-8598(pISSN)
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  • 2288-3371(eISSN)
한국어류학회 (The Ichthyological Society of Korea)
권호 표지

Dexamethasone 투여가 넙치(Paralichthys olivaceus)의 marine birnavirus (MABV) 감염강도에 미치는 영향

Effects of Dexamethasone on the Burden of Marine Birnavirus (MABV) in Olive Flounder, Paralichthys olivaceus

  • Kwon, Se Ryun (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Nam, Yoon Kwon (Department of Aquaculture, Pukyong National University)
  • 투고 : 2007.03.30
  • 심사 : 2007.05.21
  • 발행 : 2007.06.30
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초록

Marine birnavirus (MABV)에 무증상적으로 감염된 넙치 (Paralichthys olivaceus) 치어에 면역억제제의 일종인 Dexamathasone을 투여하였을 때 MABV의 감염강도에 영향을 미치는가를 조사하였다. Real time PCR 분석결과 dexamethasone을 투여한 그룹이 생리식염수를 주사한 그룹 및 no handling 그룹에 비해 유의적으로 낮은 Ct 값을 나타냈으며, 또한 semi-quantitative RT-PCR 분석결과에 있어서도 dexamethasone을 주사한 그룹이 대조구 그룹들에 비해 MABV 유전자가 유의적으로 높게 증폭되는 것으로 나타났다. 이러한 결과로부터 dexamethasone 투여가 넙치 치어에 감염된 MABV의 복제를 증가시킴을 확인하였다.

The effect of dexamethasone injection on the burden of marine birnavirus (MABV) in asymptomatically infected olive flounder (Paralichthys olivaceus) fingerlings was investigated. In real time PCR analysis, the threshold cycle (Ct) value of the fish injected with dexamethasone was significantly lower than that of the fish in the PBS-injected and no-handling groups. The higher amplification of the MABV gene in the dexamethasone-injected group than the 2 control groups was confirmed also by semi-quantitative RT-PCR. The results indicate an increase of MABV burden in olive flounder fingerlings after a single injection with dexamethasone.

키워드

과제정보

연구 과제 주관 기관 : Ministry of Maritime Affairs and Fisheries

참고문헌

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