Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Parthenogenetic Activation of Porcine Oocytes and Isolation of Embryonic Stem Cells-like Derived from Parthenogenetic Blastocysts

  • Xu, X.M. (Northwest A & F University, Shaanxi Branch of National Stem cell Engineering and Technology) ;
  • Hua, J.L. (Northwest A & F University, Shaanxi Branch of National Stem cell Engineering and Technology) ;
  • Jia, W.W. (Northwest A & F University, Shaanxi Branch of National Stem cell Engineering and Technology) ;
  • Huang, W. (Northwest A & F University, Shaanxi Branch of National Stem cell Engineering and Technology) ;
  • Yang, C.R. (Northwest A & F University, Shaanxi Branch of National Stem cell Engineering and Technology) ;
  • Dou, Z.Y. (Northwest A & F University, Shaanxi Branch of National Stem cell Engineering and Technology)
  • Received : 2006.12.16
  • Accepted : 2007.05.21
  • Published : 2007.10.01
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Abstract

These experiments were carried out to optimize the parameters of electrical activation, methods of parthenogenetic activation and embryo culture in vitro and meanwhile to isolate embryonic stem cells-like (ESCs) derived from porcine parthenogenetic blastocysts (pPBs). These results showed that, as the electric field strength increased from 1.0 to 2.7 kV/cm, the cleavage rate of parthenogenetic embryos increased gradually but the rate of oocyte lysis was significantly increased when using 2.7 kV/cm field strength. The rate of cleavage in 2.2 and 2.7 kV/cm groups was significantly increased in comparison with that of the 1.0 kV/cm group. A voltage field strength of 2.2 kV/cm DC was used to investigate blastocyst development following activation with a single pulse of 30 or $60-{\mu}sec$ pulse duration. The optimum pulse duration was 30-${\mu}sec$, with a blastocyst rate of 20.7%. Multiple pulses were inferior to a single pulse for blastocyst yield (8.0% vs. 29.9) (p<0.05). For porcine oocyte parthenogenetic activation methods, the rates of cleavage (79.0% vs. 59.8%) and blastocysts (19.4% vs. 3.4%) were significantly increased in electrical activation in contrast to chemical activation with ionomycin/6-DMAP (p<0.05). Rates of cleavage and blastocyst formation in NCSU-23 and PZM-3 embryo media were higher than those of G1.3/G2.3 serial culture media, but there was no significant difference among the three groups. The total cell number of blastocysts in PZM-3 embryo culture media containing $5{\mu}g/ml$ insulin was significantly higher than that of the control (no insulin) ($44.3{\pm}9.1$ vs. $33.9{\pm}11.7$). For isolation of PESCs-like, the rates of porcine blastocysts attached to feeder layers and ICM colony formation in Method B (nude embryo culture) were better than those in Method A (intact embryo culture).

Keywords

References

  1. Allen, N. D., S. C. Barton, K. Hilton, M. L. Norris and M. A. Surani. 1994. A functional analysis of imprinting in parthenogenetic embryonic stem cells. Develop. 120:1473-1482.
  2. Augustin, R., P. Pocar, C. Wrenzycki, H. Niemann and B. Fischer. 2003. Mitogenic and anti-apoptotic activity of insulin on bovine embryos produced in vitro. Reprod. 126:91-99. https://doi.org/10.1530/rep.0.1260091
  3. Bettauser, J., E. Fordberg, M. Augenstein, L. Childs, K. Eilertsen, J. Enos, T. Forsyhe, P. Golueke, G. Jurgella, R. Koppang, T. Lesmeister, K. Mallon, G. Mell, P. Misica, M. Pace, M. Pfister, N. Strelchenko, G. Voelker, S. Watt, S. Thompson and M. Bishop. 2000. Production of cloned pigs from in vitro systems. Nat. Biotechnol. 18:1055-1059. https://doi.org/10.1038/80242
  4. Bing, Y. Z., L. Che, Y. Hirao, N. Takenouchi, H. Rodriguezmartinez and T. Nagai. 2003. Parthenogenetic activation and subsequent development of porcine oocytes activated by a combined electric pulse and butyrolactone I treatment. J. Reprod. Dev. 49(2):159-166. https://doi.org/10.1262/jrd.49.159
  5. Cha, S. K., N. H. Kim, S. M. Lee, C. S. Baik, H. T and Lee, K. S. Chung. 1997. Effect of cytochalasin B and cycloheximide on the activation rate, chromosome constituent an in vitro development of porcine oocytes following parthenogenetic stimulation. Reprod. Fertil. Dev. 9(4):441-446. https://doi.org/10.1071/R96078
  6. Chen, L. R., Y. L. Shiue, L. Bertolini, J. F. Medrano, R. H. Bondurant and G. B. Anderson. 1999. Establishment of pluripotent cell lines from porcine preimplantation embryos. Theriogrnol. 52:195-212. https://doi.org/10.1016/S0093-691X(99)00122-3
  7. Cibelli, J. B., K. A. Grant, K. B. Chapman, K. Cunniff, T. Worst, H. L. Green, S. J. Walker, P. H. Gutin, L. Vilner, V. Tabar, T. Dominko, J. Kane, P. J. Wettstein, R. P. Lanza, L. Studer, K. E. Vrana and M. D. West. 2002. Parthenogenetic stem cells in nonhuman primates. Sci. 295:819. https://doi.org/10.1126/science.1065637
  8. Collas, P., R. Fissore and J. M. Robl. 1993. Preparation of nuclear transplant embryos by electroportation. Anal. Biochem. 208:1-9. https://doi.org/10.1006/abio.1993.1001
  9. Fan, H. Y., L. J. Huo, X. Q. Meng, Z. S. Zhong, Y. Hou, D. Y. Chen and Q. Y. Sun. 2003. Involvement of cacium/calmodulindependent protein kinase II (CaMKII) in meiotic maturation and activation of pig oocytes. Biol. Reprod. 69(5):1552-64. https://doi.org/10.1095/biolreprod.103.015685
  10. Gardner, D. K., W. B. Schoolcraft, L. Wagley, T. Schlenker, J. Stevens and J. Hesla. 1998. A prospective randomized trail of blastocyst culture and transfer in in vitro fertilizatioin. Hum. Reprod. 13:3434-3440. https://doi.org/10.1093/humrep/13.12.3434
  11. Ghandi, A. P., M. Lane, D. K. Gardner and R. L. Krisher. 2001. Substrate utilization in porcine embryos cultured in NCSU-23 and G1.2/G2.2 sequential culture media. Mol. Reprod. Dev. 58:269-275. https://doi.org/10.1002/1098-2795(200103)58:3<269::AID-MRD4>3.0.CO;2-L
  12. Grupen, C. G., J. C. Mau, S. M. McIIfatrick, S. Maddocks and M. B. Nottle. 2002. Effect of 6-dimethylaminopurine on electrically activated in vitro matured porcine oocytes. Mol. Reprod. Dev. 62(3):387-396. https://doi.org/10.1002/mrd.10126
  13. Grupen, C. G., P. J. Verma, Z. T. Du, S. M. McIIfatrick, R. J. Asluman and M. B. Nottle. 1999. Activation of in vivo- and in vitro-derived porcine oocytes by using multiple electrical pulses. Rerpod. Fertil. Dev. 11(7):457-62. https://doi.org/10.1071/RD00033
  14. Hossein, M. S., Y. W. Kim, S. M. Park, O. J. Koo, M. A. Hashem, D. P. Bhandari, Y. W. Jeong, S. Kim, J. H. Kim, E. G. Lee, S. W. Park, S. K. Kang, B. C. Lee and W. S. Hwang. 2007. Antioxidant fovors the developmental competence of porcine parthenogenotes by reducing reactive oxygen species. Asian-Aust. J. Anim. Sci. 20(3):334-339. https://doi.org/10.5713/ajas.2007.334
  15. Hwang, S., E. J. Choi, S. You, Y. J. Choi, K. S. Min and J. T. Yoon. 2006. Development of bovine nuclear transfer embryos using life-span extended donor cells transfected with foreign gene. Asian-Aust. J. Anim. Sci. 19(11):1574-1579. https://doi.org/10.5713/ajas.2006.1574
  16. Hyun, S., G. Lee, D. Kim, H. Kim, S. Lee, D. Nam, Y. Jeong, S. Kim, S. Yeom, S. Kang, J. Han, B. Lee and W. Hwang. 2003. Production of nuclear transfer-derived piglets using porcine fetal fibroblasts transfected with the enhanced green fluorescent protein. Biol. Reprod. 69:1060-1068. https://doi.org/10.1095/biolreprod.102.014886
  17. Im, G. S., L. Lai, Z. Liu, Y. Hao, D. Wax, A. Bonk and R. S. 2004. Prather. In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres. Theriogenol. 61:1125-1135. https://doi.org/10.1016/j.theriogenology.2003.06.006
  18. Jang, H. Y., H. S. Kong, K. D. Choi, G. J. Jeon, B. K. Yang, C. K. Lee and H. K. Lee. 2005. Effects of melatonin on gene expression of IVM/IVF porcine embryos. Asian-Aust. J. Anim. Sci. 18(1):17-22. https://doi.org/10.5713/ajas.2005.17
  19. Koo, D. B., N. H. Kim, H. T. Lee and K. S. Chung. 1997. Effects of fetal calf serum, amino acids, vitamins and insulin on blastocoel formation and hatching of in vitro and IVM/IVF derived porcine embryos developing in vitro. Theriogenol. 48:791-802. https://doi.org/10.1016/S0093-691X(97)00302-6
  20. Krisher, R. L., M. Lane and B. D. Bavister. 1993. Development competence and metabolism of bovine embryos cultured in semi defined and defined culture media. Biol. Reprod. 60:1345-1352. https://doi.org/10.1095/biolreprod60.6.1345
  21. Lee, G. S., H. S. Kim, S. H. Hyun, H. Y. Jeon, D. H. Nam, Y. W. Jeong, S. Kim, J. H. Kim, J. Y. Han, C. Ahn, S. K. Kang, B. C. Lee and W. S. Hwang. 2005. Production of transgenic cloned piglets from genetically transformed fetal fibroblasts selected by green fluorescent protein. Theriogenol. 63:973-991. https://doi.org/10.1016/j.theriogenology.2004.04.017
  22. Lee, J. W., X. Tian and X. Yang. 2004. Opeimization of parthenogenetic activation protocol in porcine. Mol. Reprod. Dev. 68(1):51-57. https://doi.org/10.1002/mrd.20043
  23. Lee, Y. S., S. Ock, S. K. Cho, B. G. Jeon, T. Y. Kang, S. Balasubramanian, S. Y. Choe and G. J. Rho. 2007. Effect of donor cell types and passages on preimplantation development and apoptosis in porcine cloned embryos. Asian-Aust. J. Anim. Sci. 20(5):711-717. https://doi.org/10.5713/ajas.2007.711
  24. Li, M., D. Zhang, Y. Hou, X. F. Sun, Q. Sun and W. H. Wang. 2003. Isolation and culture of embryonic stem cells from porcine blastocysts. Mol. Reprod. Dev. 65:429-434. https://doi.org/10.1002/mrd.10301
  25. Liu, L. and R. M. Moor. 1997. Factors affecting electrical activation of porcine oocyte matured in vitro. Anim. Reprod. Sci. 48(1):67-80. https://doi.org/10.1016/S0378-4320(97)00044-4
  26. Liu, L. and X. Yang. 1999. Interplay of maturation-promoting factor and mitogen-acivated protein kinase inactivation during metaphase-to-interphase transition of activated bovine oocytes. Biol. Reprod. 61:1-7. https://doi.org/10.1095/biolreprod61.1.1
  27. Loi, P., S. Ledda, J. J. Fulka, P. Cappai and R. M. Moor. 1998. Development of parthenogenetic and cloned ovine embryos:effect of activation protocols. Biol. Reprod. 58:1177-1187. https://doi.org/10.1095/biolreprod58.5.1177
  28. Moore, K and J. A. Piedrahita. 1996. Effects of heterologous hematopoietic cytokines on in vitro differentiation of culture porcine inner cell masses. Mol. Reprod. Dev. 45(2):139-144. https://doi.org/10.1002/(SICI)1098-2795(199610)45:2<139::AID-MRD5>3.0.CO;2-U
  29. Onishi, A., M. Iwamoto, T. Akita, S. Mikawa, K. Takeda, T. Awata, H. Hanada and A. C. F. Perry. 2000. Pig cloning by microinjection of fetal fibroblast nuclei. Sci. 289:1188-1190. https://doi.org/10.1126/science.289.5482.1188
  30. Parther, R. S. 2000. Pigs is pigs. Sci. 12:395-397.
  31. Piedrahita, J. A., G. B. Amderson and R. Bondv. 1990. Influence of feeder layer type on the efficiency of isolation of porcine embryo-derived cell lines. Theriogenol. 34(5):866-877.
  32. Robertson, E. J. 1987. Embryo-derived stem cell lines. In: Teratocarcinomas and Embryonic Stem Cells: A practical approach. IRL Press Ltd. Oxford. pp. 71-112.
  33. Somfai, T., M. Ozawa, J. Noguchi, H. Kaneko, K. Ohnuma, N. W. Karja, M. Fahrudin, N. M aedomari, A. Dinnyes, T. Nagai and K. Kikuchi. 2006. Diploid porcine parthenotes produced by inhibition of first polar body extrusion during in vitro maturation of follicular oocytes. Reprod. 132(4):559-570. https://doi.org/10.1530/rep.1.01216
  34. Stefanello, J. R., M. H. Barreta, P. M. Porciuncula, J. N. Arruda, J. F. Oliveira, M. A. Oliveira and P. B. Goncalves. 2006. Effect of angiotensin II with follicle cells and insulin-like growth factor-I or insulin on bovine oocyte maturation and embryo development. Theriogenol. 66(9):2068-2076. https://doi.org/10.1016/j.theriogenology.2006.06.005
  35. Strojek, R. M., M. A. Reed, J. A. Hoover and T. E. Wagner. 1990. A method for cultivating morphologically undifferentiated embryonic stem cells from porcine blastocysts. Theriogrnol. 33(4):901-913. https://doi.org/10.1016/0093-691X(90)90825-E
  36. Swain, J. E., C. L. Bormann and R. L. Krisher. 2001. Development and viability of in vitro derived porcine blastocysts cultured in NCSU23 and G1.2/G2.2 sequential medium. Theriogrnol. 56:459-469. https://doi.org/10.1016/S0093-691X(01)00577-5
  37. Vitullo, A. D. and J. P. Ozil. 1992. Repetitive calcium stimuli drive meiotic resumption and pronuclear development during mouse oocyte activation. Dev. Biol. 151:128-136. https://doi.org/10.1016/0012-1606(92)90220-B
  38. Vrana, K. E., J. D. Hipp, A. M. Goss, B. A. McCool, D. R. Riddle, S. J. Walker, P. J. Wettstein, L. P. Studer, V. Tabar, K. Cunniff, K. Chapman, L. Vilner, M. D. West, K. A. Grant and J. B. Cibelli. 2003. Nonhuman primate parthenogenetic stem cells. Proc. Natl. Acad. Sci. USA. 100:11911-11916. https://doi.org/10.1073/pnas.2034195100
  39. Wang, L., E. Duan, L. Y. Sung, B. S. Jeong, X. Z. Yang and C. X. Tian. 2005. Generation and Characterization of Pluripotent Stem Cells from Cloned Bovine Embryos. Biol. Reprod. 73:149-155. https://doi.org/10.1095/biolreprod.104.037150
  40. Wang, W. H., L. R. Abeydeera, R. S. Prather and B. N. Day. 1998. Functional analysis of activation of porcine oocytes by spermatozoa, calcium ionophore, and electrical pulse. Mol. Reprod. Dev. 51:346-353. https://doi.org/10.1002/(SICI)1098-2795(199811)51:3<346::AID-MRD15>3.0.CO;2-0
  41. Wheeler, M. B. 1994. Development and validation of swine embryonic stem cells. Report. Fertile. Dev. 6:563-568. https://doi.org/10.1071/RD9940563
  42. Yamazaki, W., C. R. Ferreira, S. C. Meo, C. L. Leal, F. V. Meirelles and J. M. Garcia. 2005. Use of strontium in the activation of bovine oocytes reconstructed by somatic cell nuclear transfer. Zygote. 13(4):295-302. https://doi.org/10.1017/S0967199405003333
  43. Yi, Y. J. and C. S. Park. 2005. Parthenogenetic development of porcine oocytes treated by ethanol, cycloheximide, cytochalasin B and 6-dimethylaminopurine. Anim. Reprod. Sci. 86(3-4):297-304. https://doi.org/10.1016/j.anireprosci.2004.07.007
  44. Yoshioka, K., T. Suzuki, A. Tanaka, I. M. K. Anas and S. Iwamura. 2002. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol. Reprod. 66:112-119. https://doi.org/10.1095/biolreprod66.1.112
  45. Zhu, J., E. E. Telfer, J. Fletcher, A. Springbett, J. R. Dobrinsky, P. A. De Sousa and I. Wilmut. 2002. Improvement of an electrical activation protocol for porcine oocytes. Biol. Reprod. 66:635-641. https://doi.org/10.1095/biolreprod66.3.635

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