Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Analysis of ITS DNA Sequences of Korean Oxalis Species (Oxalidaceae)

한국산 괭이밥속(Oxalis) 식물 ITS DNA 염기서열 분석

  • Koo, Jachoon (Division of Science Education, Chonbuk National University) ;
  • Chae, Mi Suk (Division of Science Education, Chonbuk National University) ;
  • Lee, Jeoung-Ki (Division of Science Education, Chonbuk National University) ;
  • Whang, Sung Soo (Division of Science Education, Chonbuk National University)
  • 구자춘 (전북대학교 과학교육학부) ;
  • 채미숙 (전북대학교 과학교육학부) ;
  • 이종기 (전북대학교 과학교육학부) ;
  • 황성수 (전북대학교 과학교육학부)
  • Received : 2007.10.04
  • Accepted : 2007.12.04
  • Published : 2007.12.31
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Abstract

This study was conducted to know the taxonomic features of nuclear ribosomal ITS DNA sequences, ITS1, ITS3 and 5.8S regions, as to nine individuals belonging to four Oxalis species in Korea and an induced species. Sequences of the same regions of sixteen taxa deposited in GenBank were also aligned with those of Korean species as outgroups. The length of ITS sequences aligned in this study is 679 by in total. Evidences, from not only the sequence similarities and divergences but also the phylogenetic and statistical treatments with ITS sequences aligned, were useful for the taxonomy of the genus. The similarity of sequences, among both cauline and acauline taxa, is high as 89% and 95% respectively, but between cauline and acauline taxa, relatively low in the range of 64~69%. The sequence divergences, among both cauline and acauline taxa, is also high as much as 0.36~0.42, but between both cauline and both acauline taxa, low as 0.04~0.06. Two groups between cauline and acauline taxa are paraphyletic, and each group makes a single Glade with a high bootstrap value. The analysis of variance, using ITS sequence aligned, revealed that taxa are significantly different in the level of 0.5%, and O. corymbosa, an induced speices, is also separated from the Korean taxa in the Duncan analysis.

본 연구는 한국산 괭이밥 4분류군과 도입종 1분류군의 9개체를 대상으로 핵 리보솜 DNA인 5.8S, ITS1 그리고 ITS2 지역 염기서열의 분류학적 특정을 알아보기 위해 수행되었다. 군외군으로 GenBank에 축적되어 있는 16분류군의 동일지역 염기서열도 같이 정렬하여 사용하였다. 정렬 된 ITS 구간의 염기서열 길이는 679 bp 이었다. 분류군 별 유사도 및 염기서열 분기와 계통학적 및 통계학적인 분석 등의 결과는 염기서열 특징이 본 속의 분류에 유용한 것으로 나타났다. 유경종간 및 무경종간에 염기서열 유사도는 95%와 89%로 높게 나타나는 반면, 유경종과 무경종 사이는 64~69%로 비교적 낮게 나타난다. 염기서열 분기에서도 유경종과 무경종간에는 0.36~0.42로 높게 나타나며, 유경종과 유경종 또는 무경종과 무경종간에는 0.04~0.06으로 낮게 나타났다. 계통학적으로 유경종과 무경종 집단은 병계원으로 나타났으며, 두 집단은 각각 신빙성이 높은 단일 계통군을 형성한다. 정렬된 염기서열은 통계학적으로 유의성이 있으며, Duncan 사후검정에서 자주괭이밥은 한국산 분류군들에서 분리되었다.

Keywords

Acknowledgement

Supported by : 과학재단

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