Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Investigation of ${\beta}$-Lactamase-producing Multidrug-resistant Pseudomonas aeruginosa Isolated from Non-Tertiary Care Hospitals in Korea

  • Sohn, Eui-Suk (Division of Antimicrobial Resistance, Center for Infectious Disease Research, National Institute of Health) ;
  • Yoo, Jeong-Sik (Division of Antimicrobial Resistance, Center for Infectious Disease Research, National Institute of Health) ;
  • Lee, Jeom-Kyu (Division of Antimicrobial Resistance, Center for Infectious Disease Research, National Institute of Health) ;
  • Lee, Kyeong-Min (Division of Antimicrobial Resistance, Center for Infectious Disease Research, National Institute of Health) ;
  • Chung, Gyung-Tae (Division of Antimicrobial Resistance, Center for Infectious Disease Research, National Institute of Health) ;
  • Shin, Eun-Shim (Division of Antimicrobial Resistance, Center for Infectious Disease Research, National Institute of Health) ;
  • Han, Sun-Young (Division of Antimicrobial Resistance, Center for Infectious Disease Research, National Institute of Health) ;
  • Lee, Sang-Hee (Department of Biological Sciences, School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Kim, Joon (Department of Laboratory of Biochemistry, School of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Yeong-Seon (Division of Antimicrobial Resistance, Center for Infectious Disease Research, National Institute of Health)
  • Published : 2007.10.30
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Abstract

A total of 2,280 nonduplicate clinical isolates of Pseudomonas aeruginosa, obtained nationwide from Korean non-tertiary care hospitals from 2002 to 2005, were identified and their susceptibilities to aminoglycosides, antipseudomonal penicillins, carbapenems, cephalosporins, monobactams, and quinolones were studied, together with their production of ${\beta}$-lactamases. Using disk diffusion and minimum inhibitory concentration tests, it was found that 2.9% of isolates were multidrug-resistant (MDR) P. aeruginosa. An EDTA-disk synergy test, PCR amplification with specifically designed primers, and direct sequencing of the PCR products showed that the $bla_{OXA-10}$, $bla_{VIM-2}$, $bla_{OXA-2}$, $bla_{OXA-17}$, $bla_{PER-1}$, $bla_{SHV-12}$, and $bla_{IMP-1}$ genes were carried by 34.3%, 26.9%, 3.0%,3.0%, 1.5%, 1.5%, and 1.5% of 67 MDR P. aeruginosa isolates, respectively. The prevalence of MDR P. aeruginosa was three-fold higher, compared with that from the United States. More than two types of ${\beta}$-lactamase genes were carried by 10.4% of isolates. The most prevalent ${\beta}$-lactamase genes were $bla_{VIM-2}$ and $bla_{OXA-10}$. This study is the first description of MDR P. aeruginosa trom non-tertiary care hospitals in Korea and the coexistence of the $bla_{VIM-2}$, $bla_{IMP-1}$, or $bla_{PER-1} in these clinical isolates.

Keywords

References

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