Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Modeling, Synthesis, and Screening of New Bacterial Quorum-sensing Antagonists

  • Kim, Cheol-Jin (School of Chemical & Biological Engineering, Seoul National University) ;
  • Kim, Jae-Eun (School of Chemical & Biological Engineering, Seoul National University) ;
  • Park, Hyung-Yeon (Department of Chemistry, College of Natural Science, Inha University) ;
  • Mclean, Robert J.C. (Department of Biology, Texas State University) ;
  • Kim, Chan-Kyung (Department of Chemistry, College of Natural Science, Inha University) ;
  • Jeon, Jong-Ho (School of Chemical & Biological Engineering, Seoul National University) ;
  • Yi, Song-Se (School of Chemical & Biological Engineering, Seoul National University) ;
  • Kim, Young-Gyu (School of Chemical & Biological Engineering, Seoul National University) ;
  • Lee, Yoon-Sik (School of Chemical & Biological Engineering, Seoul National University) ;
  • Yoon, Je-Yong (School of Chemical & Biological Engineering, Seoul National University)
  • Published : 2007.10.30
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Abstract

A new series comprising 7 analogs of N-(sulfanyl ethanoyl)-L-HSL derivatives, 2 analogs of N-(fluoroalkanoyl)-$_L$-HSL derivatives, N-(fluorosulfonyl)-L-HSL, and 2,2-dimethyl butanoyl HSL were synthesized using a solid-phase organic synthesis method. Each of the 11 synthesized compounds was analyzed using NMR and mass spectroscopies, and molecular modeling studies of the 11 ligands were performed using SYBYL packages. Thereafter, a bacterial test was designed to identify their quorum-sensing inhibition activity and antifouling efficacy. Most of the synthesized compounds were found to be effective as quorum-sensing antagonists, where antagonist screening revealed that 10 among the 11 synthesized ligands were able to antagonize the quorum sensing of A. tumefaciens.

Keywords

References

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