Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Acinetobacter marinus sp. novo and Acinetobacter seohaensis sp. nov., Isolated from Sea Water of the Yellow Sea in Korea

  • Yoon, Jung-Hoon (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, In-Gi (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Oh, Tae-Kwang (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Published : 2007.11.30
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Abstract

Two Gram-negative, nonmotile, coccobacilli, SW-$3^T$ and SW-$100^T$, were isolated from sea water of the Yellow Sea in Korea. Strains SW-$3^T$ and SW-$100^T$ contained ubiquinone-9 (Q-9) as the predominant respiratory lipoquinone and $C_{18:1}\;{\omega}9c$ and $C_{16:0}$ as the major fatty acids. The DNA G+C contents of strains SW-$3^T$ and SW- $100^T$ were 44.1 mol% and 41.9 mol%, respectively. A neighbor-joining tree based on l6S rRNA gene sequences showed that the two isolates fell within the evolutionary radiation enclosed by the genus Acinetobacter. Strains SW-$3^T$ and SW-$100^T$ exhibited a l6S rRNA gene similarity value of 95.7% and a mean DNA-DNA relatedness level of 9.2%. Strain SW-$3^T$ exhibited l6S rRNA gene sequence similarity levels of 93.5-96.9% to the validly described Acinetobacter species and fifteen Acinetobacter genomic species. Strain SW-$100^T$ exhibited l6S rRNA gene sequence similarity levels of less than 97.0% to the other Acinetobacter species except Acinetobacter towneri DSM $14962^T$ (98.0% similarity). Strains SW-$3^T$ and SW-$100^T$ exhibited mean levels of DNA-DNA relatedness of 7.3-l6.7% to the type strains of some phylogenetically related Acinetobacter species. On the basis of phenotypic, phylogenetic, and genetic data, strains SW-$3^T$ and SW-$100^T$ were classified in the genus Acinetobacter as two distinct novel species, for which the names Acinetobacter marinus sp. novo (type strain SW-$3^T$=KCTC $12259^T$=DSM $16312^T$) and Acinetobacter seohaensis sp. novo (type strain SW-$100^T$=KCTC $12260^T$=DSM $16313^T$) are proposed, respectively.

Keywords

References

  1. Anzai, Y., H. Kim, J.-Y. Park, H. Wakabayashi, and H. Oyaizu. 2000. Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int. J. Syst. Evol. Microbiol. 50: 1563-1589
  2. Baumann, L. and P. Baumann. 1981. The marine Gramnegative eubacteria; genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes, pp. 1302-1330. In M. P. Starr, H. Stolp, H. G. Trüper, A. Balows, and H. G. Schlegel (eds.), The Prokaryotes, A Handbook on Habitats, Isolation, and Identification of Bacteria. Springer, Berlin
  3. Baumann, P., M. Doudoroff, and R. Y. Stanier. 1968. A study of the Moraxella group II. Oxidase-negative species (genus Acinetobacter). J. Bacteriol. 95: 1520-1541
  4. Bouvet, P. J. M. and P. A. D. Grimont. 1986. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffi. Int. J. Syst. Bacteriol. 36: 228-240 https://doi.org/10.1099/00207713-36-2-228
  5. Bouvet, P. J. M. and S. Jeanjean. 1989. Delineation of new proteolytic genomic species in the genus Acinetobacter. Res. Microbiol. 140: 291-299 https://doi.org/10.1016/0923-2508(89)90021-1
  6. Brisou, J. and A. R. Prevot. 1954. Etude de systématique bacterienne. X. Revision des especes reunites dans le genre Achromobacter. Ann. Inst. Pasteur (Paris) 86: 722-728
  7. Bruns, A., M. Rohde, and L. Berthe-Corti. 2001. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int. J. Syst. Evol. Microbiol. 51: 1997-2006 https://doi.org/10.1099/00207713-51-6-1997
  8. Carr, E. L., P. Kämpfer, B. K. C. Patel, V. Gürtler, and R. J. Seviour. 2003. Seven novel species of Acinetobacter isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 53: 953-963 https://doi.org/10.1099/ijs.0.02486-0
  9. Cohen-Bazire, G., W. R. Sistrom, and R. Y. Stanier. 1957. Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J. Cell. Comp. Physiol. 49: 25-68 https://doi.org/10.1002/jcp.1030490104
  10. Cowan, S. T. and K. J. Steel. 1965. Manual for the Identification of Medical Bacteria. Cambridge University Press, London
  11. Ezaki, T., Y. Hashimoto, and E. Yabuuchi. 1989. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39: 224-229 https://doi.org/10.1099/00207713-39-3-224
  12. Gerner-Smidt, P. and I. Tjernberg. 1993. Acinetobacter in Denmark. II. Molecular studies of the Acinetobacter calcoaceticus−Acinetobacter baumannii complex. Acta Pathol. Microbiol. Immunol. Scand. 101: 826-832 https://doi.org/10.1111/j.1699-0463.1993.tb00187.x
  13. Komagata, K. and K.-I. Suzuki. 1987. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol. 19: 161-207
  14. Lanyi, B. 1987. Classical and rapid identification methods for medically important bacteria. Methods Microbiol. 19: 1-67
  15. Leifson, E. 1963. Determination of carbohydrate metabolism of marine bacteria. J. Bacteriol. 85: 1183-1184
  16. Nemec, A., T. De Baere, I. Tjernberg, M. Vaneechoutte, T. J. K. Van Der Reijden, and L. Dijkshoorn. 2001. Acinetobacter ursingii sp. nov. and Acinetobacter schindleri sp. nov., isolated from human clinical specimens. Int. J. Syst. Evol. Microbiol. 51: 1891-1899 https://doi.org/10.1099/00207713-51-5-1891
  17. Nemec, A., L. Dijkshoorn, I. Cleenwerck, T. De Baere, D. Janssens, T. J. K. Van Der Reijden, P. Ježek, and M. Vaneechoutte. 2003. Acinetobacter parvus sp. nov., a small-colony-forming species isolated from human clinical specimens. Int. J. Syst. Evol. Microbiol. 53: 1563-1567 https://doi.org/10.1099/ijs.0.02631-0
  18. Nishimura, Y., T. Ino, and H. Iizuka. 1988. Acinetobacter radioresistens sp. nov. isolated from cotton and soil. Int. J. Syst. Bacteriol. 38: 209-211 https://doi.org/10.1099/00207713-38-2-209
  19. Rossau, R., A. Van Landschoot, M. Gillis, and J. De Ley. 1991. Taxonomy of Moraxellaceae fam. nov., a new bacterial family to accommodate the genera Moraxella, Acinetobacter, and Psychrobacter and related organisms. Int. J. Syst. Bacteriol. 41: 310-319 https://doi.org/10.1099/00207713-41-2-310
  20. Sasser, M. 1990. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101, MIDI, Inc., Newark, DE
  21. Stackebrandt, E. and B. M. Goebel. 1994. Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44: 846-849 https://doi.org/10.1099/00207713-44-4-846
  22. Staley, J. T. 1968. Prosthecomicrobium and Ancalomicrobium: New prosthecate freshwater bacteria. J. Bacteriol. 95: 1921-1942
  23. Tamaoka, J. and K. Komagata. 1984. Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25: 125-128 https://doi.org/10.1111/j.1574-6968.1984.tb01388.x
  24. Tjernberg, I. and J. Ursing. 1989. Clinical strains of Acinetobacter classified by DNA-DNA hybridization. Acta Pathol. Microbiol. Immunol. Scand. 97: 595-605 https://doi.org/10.1111/j.1699-0463.1989.tb00449.x
  25. Warskow, A. L. and E. Juni. 1972. Nutritional requirements of Acinetobacter strains isolated from soil, water, and sewage. J. Bacteriol. 112: 1014-1016
  26. Wayne, L. G., D. J. Brenner, R. R. Colwell, et al. 1987. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37: 463-464 https://doi.org/10.1099/00207713-37-4-463
  27. Yoon, J.-H., H. Kim, S.-B. Kim, H.-J. Kim, W. Y. Kim, S. T. Lee, M. Goodfellow, and Y.-H. Park. 1996. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int. J. Syst. Bacteriol. 46: 502-505 https://doi.org/10.1099/00207713-46-2-502
  28. Yoon, J.-H., S. T. Lee, and Y.-H. Park. 1998. Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 48: 187-194 https://doi.org/10.1099/00207713-48-1-187
  29. Yoon, J.-H., I.-G. Kim, D.-Y. Shin, K. H. Kang, and Y.-H. Park. 2003. Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int. J. Syst. Evol. Microbiol. 53: 53-57 https://doi.org/10.1099/ijs.0.02342-0