나노 에멀젼이 잇몸 염증의 예방 및 치료에 미치는 효과에 대한 연구

THE STUDY ON THE EFFECT OF NANOEMULSION FOR THE PREVENTION AND TREATMENT OF GINGIVAL INFLAMMATION

  • 채창훈 (나노큐어텍 나노-바이오 퓨전 부설연구소) ;
  • 박준우 (한림대학교 의과대학 구강악안면외과학교실)
  • Chae, Chang-Hoon (Institute of Nano-Bio Fusion Technology, NanoCureTech Inc) ;
  • Park, Jun-Woo (Dept. of Oral and Maxillofacial Surgery, College of Medicine, Hallym University)
  • 발행 : 2007.10.31

초록

We have found out the relationship of nanoemulsion containing nano vitamin C, E and propolis and gingival disease. We've confirmed effect of nanoemulsion through the experiment of in vivo and in vitro. We tested cell viability of gingival fibroblast cells by MTT assay and mRNA appearance of interleukin-$1{\beta}$, using mouse that was guided inflammation. Anti-microbacterial activity for Antibacterial effect's experiment was carried out by using S.aureus and E.coli. In addition, inflammation tissue has been observed with scanning electrical microscopy. In this study, expression of interleukin-$1{\beta}$ was decreased after adding nanoemulsion containing nanovitamin C, E and propolis. We've also obtained good results from the test of Antibacterial effect against S.aureus and E.coli. Also, swelling of inflammation tissues observed by scanning electrical microscopy has gone down. In conclusion, we have gained confidence that nanoemulsion containing nano vitamin C, E and propolis has very high Antibacterial effect against bacteria in oral. And it made us guess that inflammation of gingival reduces after decreasing interleukin-$1{\beta}$. Thus, we expect that nanoemulsion containing nano vitamin C, E and propolis gives good effects to patient having gingival disease.

키워드

참고문헌

  1. Shojiro Kitajima, et al.: Role of Cks1 Overexpression in Oral Squamous Cell Carcinomas Cooperation with Skp2 in Promoting p27 Degradation. Am J Pathol 2004;165(6):2147-2155 https://doi.org/10.1016/S0002-9440(10)63264-6
  2. Crispian Scully and Stephen Porter. Oral cancer West J Med. 2001;174(5):348-351 https://doi.org/10.1136/ewjm.174.5.348
  3. Jon Sudb: Novel Management of Oral Cancer: A Paradigm of Predictive Oncology. Clin Med Res. 2004;2(4):233-242 https://doi.org/10.3121/cmr.2.4.233
  4. E Benjamin, et al.: Malignant fibrous histiocytomas of salivary glands. Clin Pathol 1982; 35(9):946-953 https://doi.org/10.1136/jcp.35.9.946
  5. SANGEETA GAJENDRA, GUSTAVO D. CRUZ, and JAYANTH V KUMAR: Oral Cancer Prevention and Early Detection: Knowledge, Practices, and Opinions of Oral Health Care Providers in New York State. J Cancer Educ. Author manuscript; available in PMC 2007 June 20
  6. Yan-min Wu, Jie Yan, Li-li Chen, Wei-lian Sun, and Zhi-yuan Gu: Infection frequency of Epstein-Barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters. Zhejiang Univ Sci B 2006;7(11):876-883. Published online 2006 October 17 https://doi.org/10.1631/jzus.2006.B0876
  7. Uros Skaleric, et al.: Proinflammatory and Antimicrobial Nitric Oxide in Gingival Fluid of Diabetic Patients with Periodontal Disease. Infect Immun 2006;74(12):7010-7013. Published online 2006 October 2 https://doi.org/10.1128/IAI.00071-06
  8. Lorna Mason, et al.: Topical NSAIDs for chronic musculoskeletal pain: systematic review and meta-analysis. BMC Musculoskelet Disord 2004;5:28. Published online 2004 August 19
  9. Ong KS and Seymour RA: Maximizing the safety of nonsteroidal anti-inflammatory drug use for postoperative dental pain: an evidence-based approach. Anesth Prog 2003;50(2):62-74
  10. Lands L, Stanojevic S: Oral non-steroidal anti-inflammatory drug therapy for cystic fibrosis. Cochrane Datebase Syst Rev 2007; 17:(4):CD001505
  11. Anna Holdgate and Tamara Pollock: Systematic review of the relative efficacy of non-steroidal anti-inflammatory drugs and opioids in the treatment of acute renal colic. BMJ 2004 June 12; 328(7453): 1401
  12. Joshi HN: Drug development and imperfect design. Int J Pharm 2007:1;343(1-2):1-3. Epub 2007 Jul 4 https://doi.org/10.1016/j.ijpharm.2007.06.046
  13. Schrier RD, McCutchan JA, and Wiley CA: Mechanisms of immune activation of human immunodeficiency virus in monocytes/macrophages. J Virol 1993;67(10):5713-5720
  14. Garner A, Rahi AH, and Wright JE: Lymphoproliferative disorders of the orbit: an immunological approach to diagnosis and pathogenesis. Br J Ophthalmol 1983;67(9):561-569 https://doi.org/10.1136/bjo.67.9.561
  15. Nicholas P Restifo: Building better vaccines: how apoptotic cell death can induce inflammation and activate innate and adaptive immunity. Curr Opin Immunol Author manuscript; available in PMC 2007 August 29
  16. Singer RE and Buckner BA: Butyrate and propionate: important components of toxic dental plaque extracts. Infect Immun 1981; 32(2):458-463
  17. Tanzer JM, Slee AM, Kamay B, and Scheer ER: In Vitro Evaluation of Three Iodine-Containing Compounds as Antiplaque Agents. Antimicrob Agents Chemother 1977;12(1):107-113 https://doi.org/10.1128/AAC.12.1.107
  18. Tsai CC, et al.: Extraction and partial characterization of a leukotoxin from a plaque-derived Gram-negative microorganism. Infect Immun 1979;25(1):427-439
  19. Ming Jiang: Review of 'Glossary of Biotechnology and Nanobio technology Terms' by Kimball Nill. Biomed Eng Online 2007;6:5. Published online 2007 January 31
  20. Junbai Wang, et al.: Tumor classification and marker gene prediction by feature selection and fuzzy c-means clustering using microarray data. BMC Bioinformatics 2003;4:60
  21. Laura De Laporte and Lonnie D. Shea.: Matrices and Scaffolds for DNA Delivery in Tissue Engineering. Adv Drug Deliv Rev. Author manuscript; available in PMC 2007 August 16
  22. Salata OV: Applications of nanoparticles in biology and medicine. J Nanobiotechnology. 2004;2:3. Published online 2004 April 30
  23. Levine M, et al.: Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance. Proc Natl Acad Sci USA. 1996:16;93(8):3704-3709
  24. Frei B, England L, and Ames BN: Ascorbate is an outstanding antioxidant in human blood plasma. Proc Natl Acad Sci USA. 1989;86(16):6377-6381