Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Isolation and Identification of Newly Isolated Antagonistic Streptomyces sp. Strain AP19-2 Producing Chromomycins

  • Wu, Xue-Chang (Institute of Microbiology, College of Life Sciences, Zhejiang University) ;
  • Chen, Wei-Feng (Institute of Microbiology, College of Life Sciences, Zhejiang University) ;
  • Qian, Chao-Dong (Institute of Microbiology, College of Life Sciences, Zhejiang University) ;
  • Li, Ou (Institute of Microbiology, College of Life Sciences, Zhejiang University) ;
  • Li, Ping (Institute of Microbiology, College of Life Sciences, Zhejiang University) ;
  • Wen, Yan-Ping (Institute of Microbiology, College of Life Sciences, Zhejiang University)
  • Published : 2007.12.31
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Abstract

A new antagonistic strain of actinomycete, designated AP19-2, was isolated from the feces of giant pandas inhabiting the Foping National Nature Reserve in China. Cultural characteristic studies strongly suggested that this strain is a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene of strain AP19-2 evidenced profound similarity (97-99 %) with other Streptomyces strains. Two pure active molecules were isolated from a fermentation broth of Streptomyces sp. strain AP19-2 via extraction, concentration, silica gel G column chromatography, and HPLC. The chemical structures of the two related compounds (referred to as chromomycin $A_2$ and chromomycin $A_3$) were established on the basis of their Infrared spectra (IR), High Resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS), and Nuclear Magnetic Resonance (NMR) data, and by comparison with published data.

Keywords

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