대한한의학방제학회지 (Herbal Formula Science)

  • 제15권2호
  • /
  • Pages.119-126
  • /
  • 2007
  • /
  • 1229-1218(pISSN)
  • /
  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
권호 표지

한약 탕전 팩의 미생물 연구

Observation of Microorganism in Herbal Decoction mafactured by Korean Medical Clinic

  • 유영법 (한국한의학연구원 한약제제연구부) ;
  • 마진열 (한국한의학연구원 한약제제연구부) ;
  • 하혜경 (한국한의학연구원 한약제제연구부) ;
  • 황대선 (한국한의학연구원 한약제제연구부) ;
  • 김복규 (한국한의학연구원 한약제제연구부) ;
  • 신광수 (대전대학교 미생물학과) ;
  • 신현규 (한국한의학연구원 한약제제연구부)
  • Yu, Young-Beob (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Ma, Jin-Yeul (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Huang, Dae-Sun (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Kim, Bok-Kyu (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Shin, Kwang-Soo (Department of Microbiology, College of Science, Daejeon University) ;
  • Shin, Hyun-Kyoo (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine)
  • 발행 : 2007.12.30
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초록

Objectives: This study presents observation of microorganism such as total aerobic bacteria, total fungus, E. coli, Pseudonomas aerugjnosa, Staphylococcus aureus, and Salmonella typhimurium in herbal decoction manufactured by Korean medical clinic. Methods: We examined to observe microorganism using the requirements for the experimental methods recommended by FDA. For the identification, we observed microscopic methods and carried out polymerase chain reaction (PCR) and DNA purification. The purified DNA samples were analyzed by DNA sequencer. As compared with NCBI database. the results were identified by sequences similarity. Results and conclusion: 26 (55%) of 46 decoctions observed positive for microbial test. 12 (46 %) of 26 positive decoctions exceed requirement of microbial limit test. These microbial colony identified genus of Bacillus using microscopic and DNA sequencing methods.

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