The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antioxidant Effects of Rehmanniae Radix Preparat in GC-1 Cells

숙지황(熟地黃)이 남성생식세포 GC-1의 항산화에 미치는 영향

  • Park, Seong-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Chang, Mun-Seog (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Yang, Woong-Mo (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Yu, Tae-Weon (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Do-Rim (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Park, Eun-Hwa (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Ko, Eun-Bit (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Moon-Jung (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Hyu-Young (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Oh, Ji-Hoon (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Shim, Kyung-Jun (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Yoon, Ji-Won (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
  • 박성규 (경희대학교 한의과대학 처방제형학교실) ;
  • 장문석 (경희대학교 한의과대학 처방제형학교실) ;
  • 양웅모 (경희대학교 한의과대학 처방제형학교실) ;
  • 유태원 (경희대학교 한의과대학 처방제형학교실) ;
  • 김도림 (경희대학교 한의과대학 처방제형학교실) ;
  • 박은화 (경희대학교 한의과대학 처방제형학교실) ;
  • 고은빛 (경희대학교 한의과대학 처방제형학교실) ;
  • 최문정 (경희대학교 한의과대학 처방제형학교실) ;
  • 김휴영 (경희대학교 한의과대학 처방제형학교실) ;
  • 오지훈 (경희대학교 한의과대학 처방제형학교실) ;
  • 심경준 (경희대학교 한의과대학 처방제형학교실) ;
  • 윤지원 (경희대학교 한의과대학 처방제형학교실)
  • Published : 2007.12.31
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Abstract

Objectives : The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Rehmanniae Radix Preparat (RR). Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on H2O2-induced cytotoxicity by MTT assay and lipid peroixidation by malondialdehyde (MDA) formation, respectively. Results : The results showed that the extract scavenged DPPH radical in a dose-dependent manner by up to 43.1%. The extract at concentrations of 100 ${\mu}g/ml$ showed peak level of 136.5% in growth of GC-1 cell. The hydrogen peroxide-induced cytotoxicity was blocked by the extract at concentrations of 10, 50 and 100 ${\mu}g/ml$. The extract (50 and 100 ${\mu}g/ml$) also displayed a dose-dependent reduction of MDA formation on hydrogen peroxide-induced lipid peroxidation. Conclusions : In conclusion, the extract of Rehmanniae Radix Preparat has strong antioxidant activity.

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