Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지

The Effect of Antibacteria and Antioxidantion Activities from Needle Leaf Tree

침엽수 추출물의 항균.항산화 효과

  • Zhoh, Choon-Koo (Department of Chemical & Environmental Engineering, Soong Sil University) ;
  • Kim, Kyung-Rae (Department of Chemical & Environmental Engineering, Soong Sil University) ;
  • Kim, Joo-Chan (Department of Chemical & Environmental Engineering, Soong Sil University)
  • 조춘구 (숭실대학교 환경.화학공학과) ;
  • 김경례 (숭실대학교 환경.화학공학과) ;
  • 김주찬 (숭실대학교 환경.화학공학과)
  • Published : 2007.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

We studied anti-microbial and anti-oxidant activities of bio-active components in stem of Juniperus chinensis L. and leaf of Abies koreana Wilson. Those plants of needle leaf tree family were reported to contain anti-cancer compounds. The anti-bacterial activity was tested by Broth dilution method against Escherichia coli and Staphylococcus aureus. As results, Juniperus chinensis L. and Abies koreana Wilson extracts showed 17.0% and 8.5% higher anti-bacterial activity than methyl paraben, respectively. The free radical scavenging activity of Juniperus chinensis L. and Abies koreana Wilson extracts showed 45 % and 44 % at 5,000 ppm. We measured polyphenol (catechin equivalent) and flavonoids quantity. The Juniperus chinensis L. extract contained 312 mg/g of polyphenol and 105 mg/g of flavonoids. The Abies koreana Wilson extract contained 280 mg/g of polyphenol and 103.8 mg/g of flavonoids. The cytotoxicity of extracts was measured by neutral red assay. Extracts did not affect the viability of CCK-986sk cells up to a concentration of 1,250 ppm. In conclusion, these data suggest that extracts of needle leaf trees would be usefull as antiseptic agents and anti-oxidants for cosmetic products.

산업발전에 따라 환경분야가 대두됨에 따라 민감성 피부에 화장품을 사용하는데 있어 부작용이 증가하였다. 이에 따라 저자극성 방부제의 개발을 위하여 항암효과가 입증된 침엽수 중 구상나무, 향나무의 활성성분을 추출하여 항균력을 측정 평가하였다. 항균력 측정에는 Broth dilusion법이 사용되었으며, 균주는 그람 음성균인 Escherichia coli와 그람 양성균인 Staphylococcus aureus를 사용하였다. 그 결과 항균효과는 향나무와 구상나무가 625 ppm에서 methyl paraben에 비하여 각각 17.02 %, 8.5 % 더 높은 효과를 나타냈다. 항산화 효과 측정은 DPPH법, 총 폴리페놀 함량은 Folin-Denis법, 총 플라보노이드의 함량은 Nieva Moreno법을 사용하여 분석하였다. 항산화 효과에서 향나무는 5,000 ppm에서 45 %, 구상나무는 44 %의 항산화 효과를 나타냈으며 분석 결과 총 폴리페놀과 총 플라보노이드 함유량과 비례하였다. 침엽수 추출물의 세포독성실험은 피부섬유아세포인 CCK-986sk를 사용하였다. 그 결과 향나무, 구상나무가 39 ppm에서 1,250 ppm까지의 모든 농도에서 95% 이상의 높은 세포 생존률을 나타냈다. 이상을 종합해 본 결과 향나무 줄기, 구상나무 잎은 합성물질을 대체할 수 있는 방부제 및 보조 항산화제로서 사용이 가능하다고 할 수 있다.

Keywords

References

  1. K. J. Lee and K. H. Row, Comparison of extraction methods for aglycone isoflavones from Korean soybean, Korean J. Biotechnol. Bioeng., 19, 421 (2004)
  2. Y. L. Hong, M. H. Kim, C. Ahn, H. Y. Lee, and J. D. Kim, Studies on the biological activities of the extract from Hovenia dulcis Thunb, Inst. Agr. Sci. 11, 1 (2000)
  3. M. K. Lee, Y. G. Kim, S. W. An, M. H. Kim, J. H. Lee, and H. Y. Lee, Biological activities of Hovenia dulcis Thunb, Korean J. Meddicinal Crop. Soc., 7, 185 (1999)
  4. S. R. Marouchoc, Cosmetic preservation, Cosmet. Technol. 2, 38 (1980)
  5. D. K. Brannan, Cosmetic microbiology, 593, Sandiego academic press, Sandiago, USA (1993)
  6. I. Y. Kim, S. W. Jung, H. C. Ryoo, and C. K. Zhoh, Anti-aging effects of the extracts from leaf. stem, fruit and seed of Yew (Taxus cuspidata Sieb) by solvent extraction method, J. Soc. Cosmet. Scientist Korea, 30(2), 211 (2004)
  7. C. K. Zhoh, B. N. Kim, S. H. Hong, and C. G. Han, The antimicrobial effects of natural aromas for substitution of parabens, J. Soc. Cosmet. Scientist Korea, 28(1), 166 (2002)
  8. Y. G. Kim, Studies on the extractives of Abies koreana Wilson-concerned with lignans, J. Korean Wood Sci. Tech., 25(4), 1 (1997)
  9. R. Crawford, Ecological case stories of plant adaptation to adversity, 253, Blackwell scientific publications, England (1989)
  10. S. Jodai and S. Kazuhiko, Wood science chemistry 8th, 69, Kaijousya, Tokyo (1993)
  11. T. H. Kim, A study of physiology comparison of Juniperus chinesis L. and Juniperus rigida Sieb. Extract, NIAST, 11, 174 (2000)
  12. V. Bondet, W. Brand-wiliams, and C. Berset, Kinetics and mechanisms of antioxidnant activity using the DPPH free radical method, Lebensm. Wiss. u. Technol., 30, 609 (1979) https://doi.org/10.1006/fstl.1997.0240
  13. O. Folin and W. Denis, On phosphotungastic phosoho mdybdic compounds as color reagents, J. Biol. Chem., 12, 239 (1912)
  14. M. M. Nivea, A. R. Sampietro, and M. A. Vattuone, Comparison of the free radical-scavenging activity of propolis from several regions of Argentina, J. Ethnopharmacol., 71, 109 (1999) https://doi.org/10.1016/S0378-8741(99)00189-0