The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Screening of GLA (γ-Linolenic Acid) from Fungi by Gas Chromatography and Mass Spectroscopy

Gas Chromatograph-Mass Spectrometer를 이용한 γ-Linolenic Acid(GLA) 생성 균주탐색 및 확인

  • Kim, Jung-Bong (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Kim, Kyung-Hwan (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Hong, Seung-Beom (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Park, Jong-Sug (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Lee, Jong-Yeoul (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Kim, Sam-Sun (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Bae, Shin-Chul (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Cho, Kang-Jin (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Lee, Dong-Jin (College of Bio-resources Science, Dankook University)
  • 김정봉 (농촌진흥청 농업생명공학연구원) ;
  • 김경환 (농촌진흥청 농업생명공학연구원) ;
  • 홍승범 (농촌진흥청 농업생명공학연구원) ;
  • 박종석 (농촌진흥청 농업생명공학연구원) ;
  • 이종렬 (농촌진흥청 농업생명공학연구원) ;
  • 김삼선 (농촌진흥청 농업생명공학연구원) ;
  • 배신철 (농촌진흥청 농업생명공학연구원) ;
  • 조강진 (농촌진흥청 농업생명공학연구원) ;
  • 이동진 (단국대학교 식량자원학과)
  • Published : 2007.12.31
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Abstract

In order to select ${\gamma}-Linolenic$ acid (GLA)-producing fungi, a total of forty-four strains of 4 genera such as Phytophthora, Pythium, Mucor and Rhizopus were obtained from Koran Agricultural Culture Collection (KACC) and then analysed by using GC-FID and GC-MS. GLA was detected on 39 fungal strains, and the highest rate of GLA was found as 24.8% of total fatty acids on Mucor hiemalis f. sp. hiemalis KACC 40264. Total GLA content of Zygomycota was comparatively high - Mucor (14.2%) and Rhizopus (14.3%), whereas that of Oomycetes was low - Phytophthora (3.3%) and Pythium (3.0%). Moreover, total fatty acids of the Zygomycota fungi such as Mucor (15.4 mg/100 ml) and Rhizopus (7.1 mg/100 ml) were higher compared with the Oomycetes such as Phytophthora (2.6 mg/100 ml) and Pythium (4.5 mg/100 ml). Thus, two genera such as Mucor and Rhizopus have higher potential as an useful microbial resource. The total fatty acid content varies even within the strains of the same genus e.g. Mucor. M. blumbeus KACC 40935 showed the highest values on productivity (18.2%) of GLA and total fatty acid contents (50.8 mg/100 ml liquid medium).

가스크로마토그라프와 질량분석기 (GC-MS)를 이용하여 감마리놀렌산(GLA) 생산 균주를 선발하였다. KACC에 보존 중인 Phytophthora 속, Pythium 속 및 Mucor 속 및 Rhizopus 속 등 4속에 속하는 44개 균주를 GC-FID를 통하여 GLA를 분석하였다. 4속 39종의 곰팡이 균주에서 GLA를 확인하였으며, M. hiemalis f. sp. hiemalis KACC 40264 균주에서는 GLA의 조성비가 전체 지방산의 24.8%로서 가장 높은 비율을 나타냈다. 속간에 전체적인 GLA 함량은 Mucor 속(14.2%)과 Rhizopus 속(14.3%)의 Zygomycota가 상대적으로 높은 함량을, 그리고 Phytophthora 속(3.3%)과 Pythium 속(3.0%)의 Oomycota는 낮은 함량을 나타냄으로서 뚜렷한 양분현상을 나타냈다. 지방산총량에서도 Mucor 속(15.4 mg/100 ml)과 Rhizopus 속(7.1 mg/100 ml)가 Phytophthora 속(2.6 mg/100 ml)와 Pythium 속(4.5 mg/100 ml)보다 높게 나타나서 전체적으로 지방산을 생산하는 유용한 균류자원으로 평가되었다. Mucor 속 내에서도 계통별로 큰 차이를 나타냈는데 18.2%의 GLA를 생성함으로 지방산총량이 50.8 mg/100 ml까지 이른 Mucor blumbeus KACC 40935가 실제적으로 가장 활력이 좋은 균주로 평가되었다.

Keywords

References

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