Antibacterial Effect of Fermented Rice Water against Food-borne Bacteria in Kitchen Towel

행주 내 식품위해미생물에 대한 쌀뜨물 발효액의 살균 효과

  • Ha, Ji-Hyoung (Department of Food Science and Technology, Chung-Ang University) ;
  • Lee, Yu-Si (Department of Food Science and Technology, Chung-Ang University) ;
  • Lee, Seok-Joo (Department of Food Science and Technology, Chung-Ang University) ;
  • Hwang, Sun-Soon (Korea Food & Drug Administration) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University)
  • Published : 2007.12.31

Abstract

This study was conducted to seek optimum concentration and treatment time of fermented rice water (Rizen) to disinfect food-borne bacteria in kitchen towel. $2.65\;log_{10}cfu/g$ of E. coli was reduced when double or triple diluted fermented rice water was treated during 2 hours. In case of concentrated fermented rice water, crude and double diluted solutions showed complete sterilization after 2 and 5 hours, respectively. On the other hand, triple, quadruple, quintuple diluted solutions needed 24 hours for complete sterilization. The effect of fermented rice water as a disinfectant was compared with water, detergent and chlorine treatment against E. coli and S. Typhimurium contaminated in kitchen towel. The initial number of E. coli in untreated kitchen towel were $5.0\;log_{10}cfu/g$ and were decreased to 4.32, 3.70 and 3.02 by treatments of water, detergent and chlorine, respectively. The double and triple diluted fermented rice water and double diluted concentrated fermented rice water also reduced the E. coli in kitchen towel to 2.43, 2.30 and $1.88\;log_{10}cfu/g$, respectively. The initial number of S. Typhimurium in untreated kitchen towel were $4.80\;log_{10}cfu/g$ and were decreased to 4.12, 3.58 and 2.81 by treatments of water, detergent and chlorine, respectively. The double and triple diluted fermented rice water and double diluted concentrated fermented rice water also reduced the E. coli in kitchen towel to 2.14, 2.03 and $1.59\;log_{10}cfu/g$, respectively. Consequently, the fermented rice water is thought to be a good disinfectant to reduce food-borne bacteria like E. coli and Salmonella contaminated in kitchen towel.

본 연구는 현재 유통 판매되고 있는 쌀뜨물 발효액 라이젠의 살균소독력을 평가하기 위해 최적 조건 탐색 연구 및 오염된 행주에 대한 일반적인 세척 및 소독제들과의 살균력 비교 실험을 실시하였다. 라이젠은 2 또는 3배 희석 용액에서 2시간 처리하였을 때 $2.65\;log_{10}cfu/g$의 E. coli를 감소시켜 가장 우수한 효과를 나타내었다. 또한 라이젠 농축액의 경우 원액과 2배 희석한 용액에서 각각 2, 5시간 처리하였을 때 완전 살균효과를 나타내었다. 반면 $3{\sim}5$배 희석한 용액은 24시간 동안 처리해야만 완전 살균이 가능하였다. 행주에 오염된 E. coli에 대한 라이젠 및 농축액의 살균소독력을 비교 평가하기 위해 무처리, 물세척, 합성세제(15 ppm), 차아염소산나트륨(200 ppm)으로 처리한 결과 행주 내 잔여 균수가 각각 5.05, 4.32, 3.70, $3.02\;log_{10}cfu/g$로 나타난 반면 라이젠 2, 3배 희석액, 농축액 원액, 2배 희석액은 각각 2.30, 2.43, 0, $1.88\;log_{10}cfu/g$로 나타나 라이젠은 생균제로서 우수한 살균소독 효과를 보여주었다. 또한 행주에 오염된 S. Typhimurium을 대상으로 살균 실험을 실시한 결과 E. coli의 결과와 유사한 결과를 보여주었다. 행주 내 잔여 균수는 무처리에서 4.80, 물세척 처리에서 4.12, 합성세제 처리에서 3.58, 차아염소산나트륨 처리에서 $2.81\;log_{10}cfu/g$로 나타났다. 라이젠 2, 3배 희석액 및 농축액 2배 희석액은 2.03, 2.14, $1.59\;log_{10}cfu/g$로 나타났으며 농축액 원액은 완전 살균효과를 보여주었다. 본 연구 결과 쌀뜨물 발효액은 생균제로서 행주내 대장균, 살모넬라 등 식품위해미생물 제거를 위한 살균제로서 효과가 우수하였다.

Keywords

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