Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Expression of Endometriosis Related Genes in the Shed Endometrial Tissues from Menstrual Blood

생리혈에 존재하는 자궁내막조직에서 자궁내막증 관련 유전자의 발현 양상

  • Park, Chan-Woo (Division of Reproductive Endocrinology & Infertility, Department of Obstetrics and Gynecology, Cheil General Hospital Kwandong University College of Medicine) ;
  • Jun, Jin-Hyun (Laboratory of Reproductive Biology & Infertility Cheil General Hospital Kwandong University College of Medicine) ;
  • Koong, Mi-Kyoung (Division of Reproductive Endocrinology & Infertility, Department of Obstetrics and Gynecology, Cheil General Hospital Kwandong University College of Medicine) ;
  • Song, In-Ok (Division of Reproductive Endocrinology & Infertility, Department of Obstetrics and Gynecology, Cheil General Hospital Kwandong University College of Medicine)
  • 박찬우 (관동대학교 의과대학 제일병원 산부인과 생식내분비 및 불임분과) ;
  • 전진현 (관동대학교 의과대학 제일병원 산부인과 생식생물학 및 불임연구실) ;
  • 궁미경 (관동대학교 의과대학 제일병원 산부인과 생식내분비 및 불임분과) ;
  • 송인옥 (관동대학교 의과대학 제일병원 산부인과 생식내분비 및 불임분과)
  • Published : 2007.12.31
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Abstract

Objective: This study was performed to investigate the expressions of endometriosis related genes in shed endometrial tissues from menstrual blood of patients with or without endometriosis. Methods: The shed endometrial tissues were collected on 2$^{nd}$ or 3$^{rd}$ day of menstrual cycle with Wallace catheter in patients with endometriosis (n=16) and without endometriosis (n=26). The mRNA expressions of twelve kinds of endometriosis related genes were compared between two groups using semi-quantitative RT-PCR. Results: The collected shed endometrium was confirmed by histological observation. Expressions of telomerase, c-kit and aromatase mRNA were not detected by RT-PCR in shed endometrial tissues. The mRNA expressions of apoptosis related genes (fas, fas ligand, bcl-2, bax), stem cell factor, estrogen receptor-$\alpha$/$\alpha$, endometriosis protein-I and secretory leukocyte protease inhibitor gene were similar between shed endometrial tissues with endometriosis and without endometriosis. Conclusion: We could not find the difference of mRNA expressions of tested endometriosis related genes between shed endometrial tissues with or without endometriosis by semi-quantitative RT-PCR analysis. It may be related to the dynamical changes of gene expressions in the endometrium with menstrual cycle.

목 적: 본 연구에서는 생리혈에 존재하는 탈락된 자궁내막조직에서의 자궁내막증 관련 유전자들의 발현 양상과 자궁내막증 병태생리와의 관련성을 살펴보고자 하였다. 연구방법: 자궁내막증으로 확진된 환자 (n=16)와 정상 대조군 (n=26)에서 생리주기 2$\sim$3일째 Wallace catheter로 채취한 생리혈로부터 탈락된 자궁내막조직을 분리하였다. 기존의 연구들에서 보고된 12종류의 자궁내막증 관련 유전자들의 mRNA 발현 양상을 semi-quantitative RT-PCR 방법으로 비교, 분석하였다. 결 과: 생리혈에서 분리한 탈락된 자궁내막조직은 조직학적 관찰을 통해 자궁내막조직임을 확인하였다. 총 12가지 종류의 자궁내막증 관련 유전자에 대한 RT-PCR 분석에서 telomerase, c-kit, aromatase등의 mRNA 발현이 관찰되지 않았다. 세포사멸 (apoptosis)과 관련성이 있는 fas, fas ligand, bcl-2, bax 유전자와 stem cell factor, ER-$\alpha$/$\beta$, endometriosis protein-I, secretory leukocyte protease inhibitor 등의 mRNA 발현 양상은 자궁내막증으로 확진된 환자군과 대조군에서 통계적으로 유의한 차이를 나타내지 않았다. 결 론: 결론적으로 자궁내막증과 관련된 다양한 유전자들의 발현 양상을 생리혈에 존재하는 탈락된 자궁내막조직에서 분석하였지만 의미성이 있는 유전자를 동정하지는 못하였다. 이는 자궁내막조직의 생리학적 특징인 생리주기에 따른 유전자 발현의 역동적인 변화와 관련이 있을 것으로 생각된다.

Keywords

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