Journal of Korean Traditional Oncology (대한암한의학회지)

Korean Association of Traditional Oncology (대한암한의학회)
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The Study on the Process and Quality Control of Rhus Verniciflua Stokes Extract (Nexia)

이공산(異功散)의 혈관신생(血管新生) 및 암전이(癌轉移) 억제효과(抑制效果)에 관한 연구(硏究)

  • Kang, Chang-Hee (Department of Oriental Pathology, College of Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Kang, Hee (Department of Oriental Pathology, College of Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Shin, Hyeun-Kyoo (Korea Institute of Oriental Medicine) ;
  • ;
  • Kim, Sung-Hoon (Department of Oriental Pathology, College of Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Choi, Seung-Hoon (Department of Oriental Pathology, College of Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
  • 강창희 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 강희 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 신현규 (한국한의학연구원) ;
  • 심범상 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 김성훈 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 최승훈 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 안규석 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소)
  • Published : 2006.12.29
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Abstract

Ekongsan (EKS) was expected to have inhibitory effects on angiogenesis, considering the fact that its constituents such as Ginseng Radix, Glycyrrhizae Radix and Citri Pericarpium were reported to inhibit angiogenesis. Moreover, recently several metabolites transformed by the human intestinal microflora were reported to enhance effectiveness compared to their crude drugs. Based on these data, this study was designed to confirm whether the EKS metabolites (EKS-M) can significantly exert the anti-angiogenic and anti-metastatic activites. Hence, with EKS and EKS-M, viability assay, proliferation assay, in vitro tube formation assay, gelatin zymogram assay, in vitro invasion assay were carried out. EKS showed less toxicity in ECV304 and HT1080 cells than EKS-M. EKS-M inhibited the proliferation of HT1080 cells by 30% at 200 ${\mu}g/m{\ell}$ and 42% at 400 ${\mu}g/m{\ell}$ respectively. Also, EKS-M degraded the tube network at 200 ${\mu}g/m{\ell}$. EKS and EKS-M inhibited the expression of MMP-9 at 200 and 400 ${\mu}g/m{\ell}$in HT1080 cells. EKS reduced the invasive activity of HT1080 cells through matrigel coated transfilter at the concentration of 200 ${\mu}g/m{\ell}$ more effectively than EKS-M. These data suggest that EKS and EKS-M has anti-angiogenic and anti-metastatic activities.

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