Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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First Detection of $bla_{IMP-1}$ in Clinical Isolate Multiresistant Acinetobacter baumannii from Korea

  • Jeong Seok-Hoon (Department of Laboratory Medicine, College of Medicine, Kosin University) ;
  • Bae Il-Kwon (Department of Laboratory Medicine, College of Medicine, Kosin University) ;
  • Sohn Seung-Ghyu (Department of Biological Sciences, School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Park Kwang-Ok (Department of Laboratory Medicine, College of Medicine, Kosin University) ;
  • An Young-Jun (Department of Biological Sciences, School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Sung Kwang-Hoon (Department of Biological Sciences, School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Jang Seon-Ju (Department of Biological Sciences, School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Heo Myong-Jin (Department of Biological Sciences, School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Yang Ki-Suk (Department of Biological Sciences, School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Lee Sang-Hee (Department of Biological Sciences, School of Biotechnology and Environmental Engineering, Myongji University)
  • Published : 2006.09.01
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Abstract

Among 46 Acinetobacter baumannii isolates collected in 2004, two imipenem-resistant isolates were obtained from clinical specimens taken from patients hospitalized in Busan, Republic of Korea. Two carbapenemase-producing isolates were further investigated to determine the mechanism of resistance. These isolates were analyzed by antibiotic susceptibility testing, microbiological tests of carbapenemase activity, determination of pI, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. Two cases of infection by A. baumannii producing the IMP-1 ${\beta}$-lactamase were detected. The isolates were characterized by a modified cloverleaf synergy test and EDTA-disk synergy test. Isoelectric focusing of crude bacterial extracts revealed nitrocefin-positive bands with a pI value of 9.0. PCR amplification and characterization of the amplicons by direct sequencing indicated that the isolates carried a $bla_{IMP-l}$ determinant. The isolates were characterized by a multidrug resistance phenotype, including penicillins, extended-spectrum cephalosporins, carbapenems, and aminoglycosides. These results indicate that the observed imipenem resistance of two Korean A. baumannii isolates was due to the spread of an IMP-1-producing clone. Our microbiological test of carbapenemase activity is simple to screen class B metallo-${\beta}$-lactamase-producing clinical isolates to determine their clinical impact and to prevent further spread. This study shows that the $bla_{IMP-l}$ resistance determinant, which is emerging in Korea, may become an emerging therapeutic problem, since clinicians are advised not to use extended-spectrum cephalosporins, imipenem, and aminoglycosides. This observation emphasizes the importance of having effective control measures in Asian hospitals, such as early detection of colonized patients, isolation procedures, and a judicious use of antibiotics.

Keywords

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