KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Characterization on Fatty Acids and Amino Acids of Quality Protein Maize Lines

Quality Protein Maize 육성계통의 지방산 및 아미노산 특성

  • Published : 2006.09.01
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Abstract

This study was carried out to evaluate the nutritional properties of quality protein maize (QPM) and to select the elite lines for corn breeding. Two laboratory procedures for simultaneous identification of QPM and lysine content analysis were performed. The $BC_{1}F_{2}$ lines of KS5/QPM and KS135/QPM were analyzed with opaque-2(o2) specific SSR marker in order to differentiate the opaque-2 carrying QPM lines from the non-opaque genotypes. Although no significant difference in protein content, significant differences in lipid, ash, and crude fiber contents were observed. The composition of unsaturated fatty acid of QPM lines was slightly lower than non-QPM lines, but there was no significant difference. Sulphur-containing amino acids such as methionine and cystine showed no difference between QPM and non-QPM lines. However, lysine content of QPM lines was 38% higher than that of non-QPM lines, and the essential amino acid content of QPM lines (28.1%) was higher compared to non-QPM lines (27.1%).

Quality protein maize(QPM) 육성계통의 화학적 특성과 지방산 및 아미노산 조성을 검토하기 위하여 KS5/QPM 계통의 $BC_{1}F_{2}$ 및 KS135/QPM 계통의 $BC_{1}F_{2}$ 자식계통을 대상으로 opaque-2 specific micro-satellite marker인 umc1066를 사용하여 QPM 26계통을 선발하고 non-QPM 34계통을 공시하여 얻어진 결과를 요약하면 다음과 같다. 1. QPM계통은 non-QPM계통에 비하여 지방함량이 유의하게(p<0.05) 증가되었으나 단백질함량은 통계적 유의차가 없었다. 2. QPM 계통과 non-QPM 계통 모두 linoleic acid(Cl8:2)의 조성비가 가장 높고 oleic acid(Cl8:1), palmitic acid(Cl6:0), linolenic acid(Cl8:3), stearic acid(Cl8:0)의 순으로 지방산 조성비가 높았다. 3. QPM 계통은 non-QPM 계통에 비하여 포화지방산의 비율이 다소 높고 불포화지방산의 비율은 non-QPM 계통에 비하여 조성비가 낮았으나 통계적 유의성은 없었다. 4. QPM 계통은 non-QPM 계통에 비하여 lysine의 조성 비율이 높고 황함유 아미노산(cystine, methionine)의 비율이 증가되었다. 5. QPM 계통의 필수아미노산의 조성비율은 28.1%였고 non-QPM 계통은 27.1%로 QPM 계통이 영양학적인 면에서 유리한 것으로 판단되었다.

Keywords

References

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