Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Comparison of Culture-dependent and DGGE based Method for the Analysis of Marine Bacterial Community

배양법과 DGGE에 의한 해양세균 군집의 비교분석

  • Published : 2006.12.01
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Abstract

Seasonal variation of marine bacterial community was analyzed in the surface sea water collected from one of the stations locating at Tongyeoung coastal area, Korea. The results obtained by the culture method through identification with the VITEK Microbe ID system after pure culture in the selective medium were compared with those obtained by the DGGE based 16S rRNA PCR method. The composition of the marine bacterial community in the sea water samples harvested in September, 2004, November, 2004, January, 2005, May, 2005 and August, 2005 determined by the culture method showed 5, 5, 4, 6, and 10 strains respectively. Pseudomonas fluorescens and Acinetobacter lwoffii were detected in all seasons. The other strains were identified to be Pseudomonas stutzeri, Sphingomonas paucimobilis, Burkholderia mallei and Chryseobacterium indologenes. In contrast, the 16S rRNA PCR-DGGE method detected 10, 11, 6, 9 and 13 populations respectively in the same sea water samples and the strains were identified to be Acinetobacter lwoffii, Burkholderia mallei, Pseudomonas fluoresence, Actinobacillus ureae, Burkholderia sp., Pseudomonas stutzeri, Roseobacter sp., Vibrio parahaemolyticue, Sphingomonas paucimobilis and Rugeria algocolus. This results indicated that the DGGE based 16S rRNA PCR method was more efficient than the culture method for the grasp of the characteristics of the marine bacterial community.

2004년 9월과 11월, 2005년 1월, 5월 및 8월의 총 5회에 걸쳐 대한민국 통영연안의 1개 정점에서 표층해수를 계절별로 채취하여 해양세균 군집을 분석하였다. 선택배지에서 순수분리하여 VITEK Microbe ID system으로 동정한 배양법과 16S rRNA PCR-DGGE로 분석한 결과를 상호비교하였다. 배양법에 의한 각 계절별 해양세균 군집의 종조성은 2004년 9월은 5종, 11월은 5종, 2005년 1월은 4종, 5월은 6종 및 8월은 10종으로 조사되었고, Pseudomonas fluorescens와 Acinetobacter lwoffii는 계절과 관계없이 모두 검출되었으며, 그 외에 Pseudomonas stutzeri, Sphingomonas paucimobilis 및 Burkholderia mallei 등이 동정되었다. 16S rRNA PCR-DGGE에 의한 군집분석 결과는 2004년 9월은 10개체군, 11월은 11개체군, 2005년 1월은 6개체군, 5월은 9개체군 및 8월에는 13개체군이 조사되었고, Acinetobacter lwoffii, Burkholderia mallei, Pseudomonas fluoresence, Actinobacillus ureae, Burkholderia sp., Pseudomonas stutzeri, Roseobacter sp., Vibrio parahaemolyticus, Sphingomonas paucimobilis 및 Rugeria algocolus 등이 동정되었다. 이로부터 해양세균 군집의 분포특성을 파악하는 데는 16S rRNA PCR-DGGE가 배양법보다 더 효율적임이 판명되었다.

Keywords

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