한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제51권3호
  • /
  • Pages.248-258
  • /
  • 2006
  • /
  • 0252-9777(pISSN)
  • /
  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
권호 표지

한국 콩 육성품종의 SSR마커에 의한 유전적 다양성과 유연관계

Genetic Diversity and Relationship by SSR Markers of Korean Soybean Cultivars

  • 김성훈 (충북대학교 농업생명환경대학) ;
  • 정종욱 (농촌진흥청 농업생명공학연구원) ;
  • 문중경 (농촌진흥청 작물과학원) ;
  • 우선희 (충북대학교 농업생명환경대학) ;
  • 조용구 (충북대학교 농업생명환경대학) ;
  • 정승근 (충북대학교 농업생명환경대학) ;
  • 김홍식 (충북대학교 농업생명환경대학)
  • Kim Seong-Hun (Department of Crop science, Chungbuk National University) ;
  • Jung Jong-Wook (National Institute of Agricultural Biotechnology, RDA) ;
  • Moon Jung-Kyung (National Institute of Crop Science, RDA) ;
  • Woo Sun-Hee (Department of Crop science, Chungbuk National University) ;
  • Cho Yong-Gu (Department of Crop science, Chungbuk National University) ;
  • Jong Seung-Keun (Department of Crop science, Chungbuk National University) ;
  • Kim Hong-Sig (Department of Crop science, Chungbuk National University)
  • 발행 : 2006.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

우리나라에서 1913년부터 2002년까지 육성된 콩 91개 품종에 대하여 SSR 마커를 이용하여 유전적 다양성을 평가하고 유연관계를 분석한 결과를 요약하면 다음과 같다. 1. Primer 20개를 이용하여 분석한 결과 총 149개의 대립 인자가 확인되었고, 각 유전좌별로 최소 3개(Satt477)에서 최대 15개(Sat_036, Sat_043)의 대립인자가 확인되었으며, primer당 평균 7.5개였다. 2. 우리나라 콩 육성품종들의 유전적 다양성은 $0.424{\sim}0.905$의 범위로 평균 0.711이었고, Sat_043가 0.905로 가장 높았고 SOYHSP176가 0.424로 가장 낮았다. 3. 비가중 평균 결합법에 의한 군집분석에서 90개 품종(검정콩 4호 제외)이 7개 그룹으로 분류되었으며, I 그룹은 26품종(28.6%), IV그룹은 24품종(26.4%) 및 VI그룹은 18품종(19.8%)이 속하는 큰 그룹이었다. 4. 유전적 다양성은 육성년대별로 1970년대(0.576)에 육성된 품종이 가장 낮았고, 1990년대(0.706)에 육성된 품종들이 가장 높았고, 용도별로는 장류 및 두부용콩 품종들이 가장 높았고(0.691), 풋콩 및 올콩 품종들이 가장 낮았으며(0.514), 육성모지별로는 큰 차이가 없었다. 5. 유전적거리는 육성년대 별로는 1969년 이전과 1970년대에 육성된 품종 간에 가장 가까웠고, 1970년대와 1990년대 육성된 품종 간에는 가장 멀었다. 용도별로는 장류 및 두부용콩 품종과 풋콩 및 올콩 품종 간에 가장 멀었고, 밥밑콩 품종과 풋콩 및 올콩 품종 간이 가장 가까웠다. 육성모지별 간에는 수원과 익산에서 육성된 품종 간에 멀었고, 밀양과 익산에서 육성된 품종 간에는 가까웠다.

Genetic diversity of 91 Korean soybean cultivars was assessed with 20 simple sequence repeat (SSR). Twenty SSR loci generated a total of 149 alleles. The number of alleles for each SSR locus ranged from 3 to 15 with a mean of 7.5 alleles. Genetic diversity estimated by PIC value of 91 cultivars was ranged from 0.424 to 0.905 with an average of 0.711. Cluster analysis based on Nei's genetic distances classified 91 soybean cultivars except Geomjeongkong 4 into 7 groups. The majority groups were I, IV, and VI which included 26, 24, and 18 cultivars, respectively. Obvious differences in genetic diversity appeared to be related with the released periods of cultivars and utilization type of cultivars, but not with breeding sites. Cultivars released in 1970's and in 1990's showed the lowest and the highest genetic diversities with 0.576 and 0.706, respectively. Soybean cultivars for vegetable and early maturity showed the lowest genetic diversity with 0.514, while those for soy sauce and tofu showed the highest genetic diversity with 0.691. Genetic distance between soybean cultivar groups developed before 1969 and during 1970's was the nearest, while genetic distance between those developed in 1970's and 1990's was the furthest. Cultivar group for vegetable and early maturity showed the furthest genetic distance with cultivar group for soy sauce and tofu, while it showed the nearest genetic distance with cultivar group for cooking with rice. Genetic distance was greater between soybean cultivar groups developed in Suwon and Iksan than between those developed in Milyang and Iksan.

키워드

참고문헌

  1. Baek, I.Y., D.C. Shin, G.H. Park, H.T. Kim, and H.S. Suh, Y.H Kim, Y.J. Oh. 1997. Genetic diversity in Glycine species based on morphological characters. Kor. J. Breed. 29(2) : 249-257
  2. Bommi, P. and D.L. Ferguson. 2005. Soybean cultivar identification within a selected group using only an agarose gel system with simple sequence repeat DNA markers. Soybean Genetics Newsletter 32 : 1-5
  3. Brown-Guedira, G.L., J. A. Thompson, R. L. Nelson, and M. L. Warburton. 2000. Evaluation of genetic diversity of soybean introductions and North American ancestors using RAPD and SSR markers. Crop Sci. 40 : 815-823 https://doi.org/10.2135/cropsci2000.403815x
  4. Cansian, R. L. and S. Echeverrigaray. 2000. Discrimination among cultivars of cabbage using randomly amplified polymorphic DNA markers. Hort Sci. 35(6): 1155-1158
  5. Cho, Y.G., T. Ishii, S. M. Temnykh, X. Chen, L. Lipovich, S.R. McCouch, W.D. Park, N. Ayres, and S. Cartinhour. 2000. Diversity of microsatellites derived from genomic libraries and GeneBank sequences in rice (Oryza sativa L.). Theor. Appl. Genet. 100 : 249-257 https://doi.org/10.1007/s001220050033
  6. Cregan, P.B., T Jarvik, A. L. Bush, R. C. Shoemaker, K. G. Lark, A. L. Kahler, N. Kaya, T.T. VanToai, D. G. Lohnes, J. Chung, and J. E. Specht. 1999. An integrated genetic linkage map of the soybean genome. Crop Sci 39 : 1464-1490 https://doi.org/10.2135/cropsci1999.3951464x
  7. Cui, Z., T.E. Carter, and J.W. Burton. 2000. Genetic diversity patterns in Chinese soybean cultivars based on coefficient of parentage. Crop Sci. 40 : 1780-1793 https://doi.org/10.2135/cropsci2000.4061780x
  8. Gizlice, Z., T.E.Carter, and J.W. Burton. 1993. Genetic diversity in North American soybean : I. multivariate analysis of founding stock and relation to coefficient of parentage. Crop Sci. 33 : 614-620 https://doi.org/10.2135/cropsci1993.0011183X003300030038x
  9. Gizlice, Z., T.E. Carter, T.M. Gerig, and J.W. Burton. 1996. Genetic diversity patterns in North American public soybean cultivars based on coefficient of parentage. Crop Sci. 36 : 753-765 https://doi.org/10.2135/cropsci1996.0011183X003600030038x
  10. Han, O., J. Abe, and Y Shimamoto. 1999. Genetic diversity of soybean landraces in Korea. Korean J. Crop Sci. 44(3) : 256-262
  11. Hwang, Y.H. 2004. Historical review on soybean cultivation in korea. International Symposium on the development of Functional Soybean Varieties, New Materials, Medicine, and Foods. 1-29. Kyungbuk National University
  12. Jong, S.K., H.S. Kim, and S.Y Son. 1999. Genetic diversity using pedigree analysis in Korean soybean varieties. Korean J. Breed. 31(4) : 313-322
  13. Jung, H.S., K. Van, M.Y. Kim, and S.H. Lee. 2004. Identification of DNA using AFLP and SSR markers in soybean somaclonal variants. Korean J. Crop Sci. 49(1) : 69-72
  14. Kim, M.S., M. J. Park, J. G. Hwang, S. H. Jo, M. S. Ko, I. M. Chung, and J. I. Chung. 2004. Identification of quantitative trait loci associated with isoflavone contents in soybean seed. Korean J. Crop Sci. 49(5) : 69-72
  15. Narvel, J.M., W.R. Fehr, W.C. Chu, D. Grant, and R.C. Shoemaker. 2000. Simple sequence repeat diversity among soybean plant introductions and elite genotypes. Crop Sci. 40 : 1452-1458 https://doi.org/10.2135/cropsci2000.4051452x
  16. Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. U. S. A. 70(12) : 3321-3323
  17. Park, K.Y., Y. H. Lee, S. D. Kim, and E. H. Hong. 2000. Review and future planning for soybean breeding in Korea. Korea Soybean Digest. 17(1) : 13-26
  18. Rongwen, J.M., S. Akkaya, A. A. Bhagwat, U. Lavi, and P.B. Cregan. 1995. The use of microsatellite DNA markers for soybean genotype identification. Theor. Appl. Genet. 90 : 43-48
  19. Tatineni, V., R.G. Cantrell, and D.D Davis. 1996. Genetic diversity in elite cotton germplasm determinated by morphological characteristics and RAPDs. Crop Sci. 36 : 186-192 https://doi.org/10.2135/cropsci1996.0011183X003600010033x
  20. Tanya, P., P. Srinives, T. Toojinda, A. Vanavichit, B.K. Ha, J.S. Bae, J.K. Moon, and S.H. Lee. 2001. Evaluation of genetic diversity among soybean genotypes using SSR and SNP. Korean J. Crop Sci. 46(4) : 334-340
  21. Weising, K., P. Winter, B. Huttel, and G. Kahl. 1998. Microsatellites marker for molecular breeding. J. of Crop Production 1(1) : 113-143
  22. Yeh, F.C., R.C. Yang, T.B.J. Boyle, Z.H. Ye, and J.X. Mao. 1997. POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, Canada
  23. Yoon, M.S., Y.J. Park, J.H. Kang, H.J. Beak, M.S. Lim, J.S. Song, and Y.D. Rho. 1998. DNA Polymorphism and geographical genetic distance of soybean (Glycine max (L.) Merrill) landraces by microsatellite. Korean J. Breed. 30(2) : 192-198
  24. Zhou, X., E. Thomson, Jr. Carter, Z. Cui, S. Miyazaki, and J.W. Burton. 2002. Genetic diversity patterns in Japanese soybean cultivars based on cofficient of parentage. Crop Sci. 42 : 1331-1342 https://doi.org/10.2135/cropsci2002.1331