Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Construction of spSac3 Null Mutants Defective in mRNA Export

mRNA의 핵에서 세포질로의 이동에 관여하는 spSac3 유전자의 결실돌연변이 제조와 특성 조사

  • Kang Sook-Hee (Department of Biology, and Institute of Basic Sciences, Sungshin Women's University) ;
  • Yoon Jin-Ho (Department of Biology, and Institute of Basic Sciences, Sungshin Women's University)
  • 강숙희 (성신여자대학교 생물학과 및 기초과학 연구소) ;
  • 윤진호 (성신여자대학교 생물학과 및 기초과학 연구소)
  • Published : 2006.06.01
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Abstract

We constructed the null mutants of fission yeast Schizosaccharomyces pombe spSac3 gene that is homologous to budding yeast Saccharomyces cerevisiae SAC3 involved in mRNA export out of nucleus. Tetrad analysis showed that the spSac3 is essential for vegetative growth. The spSac3 mutants harboring pREP81X-spSac3 plasmid showed poly(A)+ RNA export defect in the presence of thiamine. These results suggest that spSac3 is also involved in mRNA export from the nucleus.

mRNA의 핵에서 세포질로의 이동에 관여하는 발아효모 Saccharomyces cerevisiae의 SAC3유전자와 유사한 분열효모 Schizosaccharomyces pombe의 유전자 (spSac3로 명명)의 결실돌연변이주 (knockout mutant)를 제조하여 그 특성을 조사하였다. S. pombe의 이배체 (diploid) 균주에서 한 spSac3 유전자만을 결실시킨 후 4분체분석 (tetrad analysis)을 수행한 결과, 이 유전자는 성장에 필수적이었다. 또한 in situ hybridization을 통해 세포 내의 poly(A)+ RNA분포를 살펴본 결과, spSac3 유전자의 결실돌연변이주는 mRNA의 핵에서 세포질로의 이동에 이상이 있었다. 이와 같은 결과들은 spSac3 유전자 역시 mRNA의 핵에서 세포질로의 이동에 매우 중요한 역할을 담당하고 있음을 시사한다.

Keywords

References

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