Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Analysis of Bacterial Diversity and Community Structure in Forest Soils Contaminated with Fuel Hydrocarbon

  • Ahn Jae-Hyung (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim Mi-Soon (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim Min-Cheol (School of Agricultural Biotechnology, Seoul National University) ;
  • Lim Jong-Sung (National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Lee Goon-Taek (National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Yun Jun-Ki (Research Institute of Technology, Samsung Corporation) ;
  • Kim Tae-Sung (Ecosystem Disturbance Assessment Division, Nature and Ecology Research Department, National Institute of Environmental Research) ;
  • Kim Tae-San (Genetic Resources Division, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Ka Jong-Ok (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2006.05.01
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Abstract

Oil spill was found in 1999 from a diesel storage facility located near the top of Baekun Mountain in Uiwang City. Application of bioremediation techniques was very relevant in removing oil spills in this site, because the geological condition was not amenable for other onsite remediation techniques. For efficient bioremediation, bacterial communities of the contaminated site and the uncontaminated control site were compared using both molecular and cultivation techniques. Soil bacterial populations were observed to be stimulated to grow in the soils contaminated with diesel hydrocarbon, whereas fungal and actinomycetes populations were decreased by diesel contamination. Most of the dieseldegrading bacteria isolated from contaminated forest soils were strains of Pseudomonas, Ralstonia, and Rhodococcus species. Denaturing gradient gel electrophoresis (DGGE) analysis revealed that the profiles were different among the three contaminated sites, whereas those of the control sites were identical to each other. Analysis of 16S rDNA sequences of dominant isolates and clones showed that the bacterial community was less diverse in the oil-contaminated site than at the control site. Sequence analysis of the alkane hydroxylase genes cloned from soil microbial DNAs indicated that their diversity and distribution were different between the contaminated site and the control site. The results indicated that diesel contamination exerted a strong selection on the indigenous microbial community in the contaminated site, leading to predominance of well-adapted microorganisms in concurrence with decrease of microbial diversity.

Keywords

References

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