Preventive Nutrition and Food Science

  • 제11권2호
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  • Pages.100-104
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  • 2006
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  • 2287-1098(pISSN)
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  • 2287-8602(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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Extracts and Essential Oil of Ledum palustre L. Leaves and Their Antioxidant and Antimicrobial Activities

  • 발행 : 2006.06.01
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초록

The in vitro antimicrobial and antioxidant activities of the essential oil and methanol extracts of Ledum palustre L. were investigated. Using GC-MS analysis, we identified 37 compounds in the essential oil, constituting 87.58% of the total oil. There are several monoterpenes, of which sabinene is the major compound ($16{\sim}17%$). There are several oxygenated monoterpenes of which terpinen-4-ol(7.6%) and myrtenal (3.5%) are the main constituents. $\beta$-Selinene, a-selinene, $\gamma$-elemene, a-caryophyllene are the main sesquiterpenes ($2{\sim}6%$ range). The oil strongly reduced the diphenylpicrylhydrazyl radical ($IC_{50}=1.56{\mu}g/mL$) formation and exhibited a hydroxyl radical scavenging effect in the $Fe^{3+}-EDTA-H_2O_2$ deoxyribose system ($IC_{50}=2.7{\mu}g/mL$), and also inhibited the nonenzymatic lipid peroxidation of rat liver homogenate ($IC_{50}=13.5{\mu}g/mL$). The polar phase of the extract showed antioxidant activity. The oil showed antimicrobial activity against Streptococcus pneumoniae, Clostridium perfringens, Candida albicans, Mycobacterium smegmatis, Acinetobacter lwoffii and Candida krusei while the water-insoluble parts of the methanolic extracts exhibited slight or no activity. This study confirms that the essential oil of Ledum palustre L. possesses antioxidant and low antimicrobial properties in vitro.

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참고문헌

  1. Baldioli M, Servili G, Montedoro GF. 1996. Antioxidant activity of tocoferols and phenolic compounds of virgin olive oil. J Am Oil Chem Soc 73: 1589-1593 https://doi.org/10.1007/BF02523530
  2. Lee KG, Shibamoto T. 2001. Antioxidant activities of volatile components isolated from Eucalyptus species. J Sci Food Agric 81: 1573-1579 https://doi.org/10.1002/jsfa.980
  3. Six P. 1994. Current research in natural food antioxidants. International News on Fats, Oils & Related Materials 5: 679-688
  4. Adams RP. 2001. Identification of Essential Oils components by Gas Chromatography/Quadrupole Mass Spec-troscopy. Allured Publishing Corporation, Illinois, USA
  5. Larson RA. 1988. The antioxidants of higher plants. Phytochemistry 27: 969-978 https://doi.org/10.1016/0031-9422(88)80254-1
  6. Yoshida H, Takagi S. 1999. Antioxidative effects of sesamol and tocopherols at various concentrations in oils during microwave heating. J Sci Food Agric 79: 220-226 https://doi.org/10.1002/(SICI)1097-0010(199902)79:2<220::AID-JSFA173>3.0.CO;2-8
  7. Narimanov AA. 1992. The reproductive capacity of male mice protected against the superlethal action of gamma radiation by the administration of a mixture of Archangelica officinalis and Ledum palustre extracts. Radiobiologiia 32: 271-275
  8. Narimanov AA, Miakisheva SN, Kuznetsova SM. 1991. The radioprotective effect of extracts of Archangelica officinalis Hoffm. and Ledum palustre L. on mice. Radiobiologiia 31: 391-393
  9. Jaenson TGT, Palsson K, Borg-Kalson AK. 2005. Evaluation of extracts and oils of tick-repellent plants from Sweden. Med Vet Entomol 19: 345-352 https://doi.org/10.1111/j.1365-2915.2005.00578.x
  10. Kuusik A, Harak M, Hiiesaar K, Metspalu L, Tartes U. 1995. Studies on insect growth regulating (IGR) and toxic effects of Ledum palustre extracts on Tenebrio molitor pupae (Coleoptera, Tenebrionidae) using calorimetric recordings. Thermochimica Acta 251: 247-253 https://doi.org/10.1016/0040-6031(94)02048-S
  11. Palsson K, Jaenson TGT. 1999. Plant products used as mosquito repellents in Guinea Bissau, West Africa. Acta Tropica 72: 39-52 https://doi.org/10.1016/S0001-706X(98)00083-7
  12. Hippokrates F. 1973. Stuttgart Hagers Handbuch der Pharmazeutischen Praxis. Hiirhammer L, ed. Springer, Berlin. p 56
  13. Baytop T. 1999. Turkiye'de bitkiler ile tedavi (treatment with plants in Turkey). Istanbul University Publications No. 3255:40, Istanbul. p 176
  14. Davis PH. 1982. Flora of Turkey and the East Aegean Islands. University Press, Edinburgh. Vol 5, p 244
  15. Robak J, Gryglewski RJ. 1988. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol 37: 837-841 https://doi.org/10.1016/0006-2952(88)90169-4
  16. Pattnaik S, Subramanyam VR, Bapaji M, Kole CR. 1997. Antibacterial and antifungal activity of aromatic constituents of essential oils. Microbios 89: 39-46
  17. Kunchandy E, Rao MNA. 1990. Oxygen radical scavenching activity of curcumin. Int J Pharm 58: 237-240 https://doi.org/10.1016/0378-5173(90)90201-E
  18. Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  19. Cuendet M, Hostettmann K, Potterat O. 1997. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta 80: 1144-1152 https://doi.org/10.1002/hlca.19970800411
  20. Bishayee S, Balasubramanian AS. 1971. Lipid peroxide formation in rat brain. J Neurochem 18: 909-920 https://doi.org/10.1111/j.1471-4159.1971.tb12020.x
  21. Hayase F, Kato H. 1984. Antioxidative components of sweet potatoes. J Nutr Sci Vitaminol 30: 37-46 https://doi.org/10.3177/jnsv.30.37
  22. Ramarathnam N, Osawa T, Namiki M, Tashiro T. 1986. Studies on the relationship between antioxidative activity of rice hull and germination ability of rice seeds. J Sci Food Agric 37: 719-726 https://doi.org/10.1002/jsfa.2740370803
  23. Sokmen A, Jones BM, Erturk M. 1999. The in vitro antibacterial activities of Turkish medicinal plants. J Ethnopharmacol 67: 79-86 https://doi.org/10.1016/S0378-8741(98)00189-5
  24. Knobloch K, Pauli A, Iberi B, Wegand H, Weis N. 1989. Antibacterial and antifungal properties of essential oil components. J Ess Oil Res 1: 119-128 https://doi.org/10.1080/10412905.1989.9697767

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