Preventive Nutrition and Food Science

  • 제11권2호
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  • Pages.94-99
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  • 2006
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  • 2287-1098(pISSN)
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  • 2287-8602(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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Protective Effects of the BuOH Fraction from Laminaria japonica Extract on High Glucose-induced Oxidative Stress in Human Umbilical Vein Endothelial Cells

  • Park, Min-Jung (Department of Food Science and Nutrition, Pusan National University) ;
  • Song, Young-Sun (School of Food and Life Science, Biohealth Product Research Center and Food Science Institute, Inje University) ;
  • Han, Ji-Sook (Department of Food Science and Nutrition, Pusan National University)
  • 발행 : 2006.06.01
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초록

This study investigated the protective effect of the butanol (BuOH) fraction from Laminaria japonica (BFLJ) extract on high glucose-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). Freeze-dried L japonica was extracted with distilled water, and the extracted solution was mixed with ethanol then centrifuged. The supernatant was subjected to sequential fractionation with various solvents. The BuOH fraction was used in this study because it possessed the strongest antioxidant activity among the various solvent fractions. To determine the protective effect of the BFLJ, oxidative stress was induced by exposing of HUVECs to the high glucose (30 mM) or normal glucose (5.5 mM) for 48 hr. Cell viability, lipid peroxidation, glutathione (GSH) concentration, and antioxidant enzyme activities such as catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-px), and glutathion reductase (GSH-re) were measured. Exposure of HUVECs to high glucose for 48 hr resulted in a significant (p<0.05) decrease in cell viability, SOD, GSH-px and GSH-re and a significant (p<0.05) increase in thiobarbituric acid reactive substances (TBARS) formation in comparison to the cells treated with 5.5 mM glucose or untreated with glucose. BFLJ treatment decreased TBARS formation and increased cell viability, GSH concentration, and activities of antioxidant enzymes including catalase, SOD, GSH-px, and GSH-re in high glucose pretreated HUVECs. These results suggest that BFLJ may be able to protect HUVECs from high glucose-induced oxidative stress, partially through the antioxidative defence systems.

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참고문헌

  1. Gulter RG, Packer LB, Eartum J, Mori A. 1995. Oxidative stress, antioxidants, aging and disease. In Oxidative stress and aging. Birkhauser Verlag, Basel, Switzerland. p 152- 163
  2. Rosen P, Nawroth PP, King G, Moller W, Tritschler HJ, Packer L. 2001. The role of oxidative stress in the onset and progression of diabetes and its complications: a sum-mary of a Congress Series sponsored by UNESCO- MCBN, the American Diabetes Association and the German Diabetes Society. Diabetes Metab Res Rev 17: 189-212 https://doi.org/10.1002/dmrr.196
  3. Wiernsperger NF. 2003. Oxidative stress: the special case of diabetes. Biofactors 19: 11-18 https://doi.org/10.1002/biof.5520190103
  4. Diabetes Control and Complications Trial Research Group. 1993. The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. N Engl J Med 329: 977-986 https://doi.org/10.1056/NEJM199309303291401
  5. Kinoshita JH. 1990. A thirty year journey in the polyol pathway. Exp Eye Res 50: 567-573 https://doi.org/10.1016/0014-4835(90)90096-D
  6. Kuusisto JL, Mykkanen K, Pyorala K, Laakso M. 1994. NIDDM and its metabolic control predict heart disease in elderly subjects. Diabetes 43: 960-967 https://doi.org/10.2337/diabetes.43.8.960
  7. Stehouwer CAD, Schaper NC. 1996. The pathogenesis of vascular complications of diabetes mellitus: One voice or many?. Eur J Clin Invest 26: 535-543 https://doi.org/10.1046/j.1365-2362.1996.1780527.x
  8. Celermajer DS. 1997. Endothelial dysfunction: Does it matter? Is it reversible?. J Am Coll Cardiol 30: 325-333 https://doi.org/10.1016/S0735-1097(97)00189-7
  9. Lorenzi M, Montisano DF, Toledo S, Barrieux A. 1986. High glucose induces DNA damage in cultured human endothelial cells. J Clin Invest 77: 322-325 https://doi.org/10.1172/JCI112295
  10. Graier WF, Simecek S, Hoebel BG, Wascher TC, Dittrich P, Kostn GM. 1997. Antioxidants prevent high D-glucose- enhanced endothelial Ca2+/cGMP response by scavenging superoxide anions. Eur J Pharmacol 322: 113-122 https://doi.org/10.1016/S0014-2999(96)00989-2
  11. Yan X, Nagata T, Fan X. 1998. Antioxidative activities in some common seaweed. Plant Foods Hum Nutr 52: 253-262 https://doi.org/10.1023/A:1008007014659
  12. Percival E, McDowell RH. 1967. Chemistry and enzymology of marine algal polysaccharides. Academic Press, New York. p 157-175
  13. Han J, Kang S, Choue R, Kim H, Leem K, Chung S, Kim C, Chung J. 2002. Free radical scavenging effect of Dios-pyros kaki, Laminaria japonica and Undaria pinnatifida. Fitoterapia 73: 710-712 https://doi.org/10.1016/S0367-326X(02)00236-8
  14. Lee KS, Bae BS, Bae MJ, Jang MA, Seo JS, Choi YS. 1999. Effect of sea tangle and metformin on lipid peroxide and antioxidants levels in diabetic rats. Korean J Nutr 32: 230-238
  15. Jin DQ, Li G, Kim JS, Yong CS, Kim JA, Huh K. 2004. Preventive effects of Laminaria japonica aqueous extract on the oxidative stress and xanthine oxidase activity in streptozotocin-induced diabetic rat liver. Biol Pharm Bull 27: 1037-1040 https://doi.org/10.1248/bpb.27.1037
  16. Wang Y, Tang X. 2005. Relationships between antioxidant activities and heat-resistant features of two Laminaria japonica strains. Ying Yong Sheng Tai Xue Bao 16: 1507- 1512
  17. Nan JX, Park EJ, Nam JB, Zhao YZ, Cai XF, Kim YH, Sohn DH, Lee JJ. 2004. Effect of Acanthopanax koreanum Nakai (Araliaceae) on-galactosamine and lipopolysaccharide- induced fulminant hepatitis. J Ethnopharmacol 92: 71-77 https://doi.org/10.1016/j.jep.2004.02.007
  18. Nagal T, Inoue R, Inoue H, Suzuki N. 2002. Scavenging capacities of pollen extracts from cistus ladaniferus on autoxidation, superoxide radicals, hydroxyl radicals and DPPH radicals. Nutr Res 22: 519-526 https://doi.org/10.1016/S0271-5317(01)00400-6
  19. Shyama R, Rao P, Muralikrishna G. 2004. Nonstarch polysaccharide-phenolic acid complexes from native and germinated cereals and millet. Food Chemistry 84: 527- 531 https://doi.org/10.1016/S0308-8146(03)00274-7
  20. Fautz R, Husen B, Hechenberger C. 1991. Application of the neutral red assay (NR assay) to monolayer cultures of primary hepatocytes: rapid colorimetric viability deter-mination for the unscheduled DNA synthesis test (UDS). Mutat Res 253: 173-179 https://doi.org/10.1016/0165-1161(91)90130-Z
  21. Fraga CG, Leibovita RM, Roeder RG. 1988. Lipid peroxidation measured as thiobarbituric-reactive substances in tissue slices: characterization and comparision with homo-genates and microsomes. Free Radic Biol Med 4: 155-161 https://doi.org/10.1016/0891-5849(88)90023-8
  22. Tietze F. 1969. Enzymic method for quantitative deter-mination of nanogram amounts of total and oxidaized glutathione: applications to mammalian blood and other tissue. Anal Biochem 27: 502-522 https://doi.org/10.1016/0003-2697(69)90064-5
  23. Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Ann Biochem 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  24. Marklund S, Marklund G. 1974. Involvement of the super-oxide anion radical in antioxidant of pyrogallol and a con-venient assay for superoxide dismutase. Eur J Biochem 47: 469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  25. Aebi H. 1984. Catalase in vitro. Methods Enzymol 105: 121-126 https://doi.org/10.1016/S0076-6879(84)05016-3
  26. Lawrence RA, Burk RF. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71: 952-958 https://doi.org/10.1016/0006-291X(76)90747-6
  27. Carlberg I, Mannervik B. 1985. Glutathione reductase. Methods Enzymol 113: 484-490 https://doi.org/10.1016/S0076-6879(85)13062-4
  28. Sevanian A, Hochstein P. 1985. Mechanism and conse-quence of lipid peroxidation in biological systems. Annu Rev Nutr 5: 365-390 https://doi.org/10.1146/annurev.nu.05.070185.002053
  29. Hochstein P, Jain SK. 1981. Association of lipid peroxidation and polymerization of membrane proteins with erythrocyte aging. Fed Proc 40: 183-188
  30. Pacifici RE, Davies KJ. 1991. Protein, lipid and DNA repair systems in oxidative stress: The free radical theory of aging revisited. Gerontology 37: 166-180 https://doi.org/10.1159/000213257
  31. Tasi CH, Chern CL, Liu TZ. 2000. Antioxidant action of glutathion: Its reaction with superoxide anion and hydroxyl radicals. J Biomed Lab Sci 12: 107-111
  32. Reed DJ, Farris MW. 1984. Glutathion depletion and susceptibility. Pharmacol Rev 36: 25S-33S
  33. Chance B, Boveris A. 1980. Hyperoxia and hydroperoxide metabolism. In Extra pulmonary manifestations of respiratory disease. Debs ER, ed. Marcel Dekker, NY. p 185- 237
  34. Husain K, Somani SM. 1998. Interation of exercise training and chronic ethanol ingestion on testicular antioxidant system in rat. J Appl Toxicol 18: 421-429 https://doi.org/10.1002/(SICI)1099-1263(199811/12)18:6<421::AID-JAT532>3.0.CO;2-R
  35. Runnegar MTC, Andrews J, Gerdes RG, Falconer IR. 1987. Injury to hepatocytes induced by a peptide toxin from the cyanobacterium Microcystis aeruginosa. Toxicon 25: 1235-1239 https://doi.org/10.1016/0041-0101(87)90142-5
  36. Alptekin N, Seckin S, Dogru-Abbasoglu S, Yelkenci F, Kocak-Toker N, Toker G, Uysal M. 1996. Lipid peroxides, glutathione, gamma-glutamylcysteine synthetase and gamma- glutamyltranspeptidase activities in several tissues of rats following water-immersion stress. Pharmacol Res 34: 167-169 https://doi.org/10.1006/phrs.1996.0084
  37. Kono Y, Fridovich I. 1982. Superoxide radicals inhibit catalase. J Biol Chem 257: 5751-5754

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