Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Immunostimulating Effect of Goamshimshinhwan on Methotrexate-induced Immunosuppressed SD Rats

고암심신환(古庵心腎丸)이 methotrexate로 유발된 SD Rat의 면역기능저하에 미치는 영향

  • Kang, Dae-Sung (Department of Internal Medicine, College of Oriental medicine, Daegu Hanny University) ;
  • Kwon, Eun-Hee (Department of Internal Medicine, College of Oriental medicine, Daegu Hanny University) ;
  • Lee, Yeon-Kyeong (Department of Internal Medicine, College of Oriental medicine, Daegu Hanny University) ;
  • Shin, Hyeon-Cheol (Department of Internal Medicine, College of Oriental medicine, Daegu Hanny University) ;
  • Kang, Seok-Bong (Department of Internal Medicine, College of Oriental medicine, Daegu Hanny University) ;
  • Park, Song-Kee (Department of Internal Medicine, College of Oriental medicine, Daegu Hanny University)
  • 강대성 (대구한의대학교 한의과대학 내과학교실) ;
  • 권은희 (대구한의대학교 한의과대학 내과학교실) ;
  • 이연경 (대구한의대학교 한의과대학 내과학교실) ;
  • 신현철 (대구한의대학교 한의과대학 내과학교실) ;
  • 강석봉 (대구한의대학교 한의과대학 내과학교실) ;
  • 박송기 (대구한의대학교 한의과대학 내과학교실)
  • Published : 2006.12.25
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Abstract

In order to investigate the effect of Goamshimshinhwan(GASSW) on SD rats with deteriorated immunity caused by methotrexate. Methotrexate was fed to the SD rats once a day for 4 days. After the immune responses of the at a dosage 1,000, 500 and 250mg/kg/10ml. and the changes on body weight and gains, spleen weight, total blood leukocyte numbers, total lymphocyte numbers, the percentage of B-cell, T-cell, CD3+CD+4 T-cell, CD3+CD8+ T-cell and CD4+/CD8+ T-cell ratios in the bolld and spleen were observed. In addition, the serum IL-2 levels and productivity of IL-2 of splenic cells were also demonstrated in this study. The changes on body weight were increased significantly in 100 and 500mg/kg of GASSW groups and the changes on body gain were increased significantly in 1000mg/kg of GASSW groups as compared with control group. The changes on the spleen weight (absolutely or relatively) were increased significantly in all GASSW groups as compared with control group. The total blood leukocyte numbers were increased significantly in 1000 and 500mg/kg of GASSW groups as compared with control group. The total lymphocyte numbers were increased significantly in all GASSW groups in the blood and increased significantly in 1000 and 500mg/kg of GASSW goups in spleen as compared with control group. The percentage of B-cell and T-cell were increased significantly in 1000mg/kg of GASSW groups in the blood and increased significantly in 1000 and 500mg/kg of GASSW groups in spleen as compared with control group. The percentage of CD3+CD4+ T-cell and the serum IL-2 levels and productivity of IL-2 of splenic cells were increased significantly in 100 and 500 mg/kg of GASSW groups in the blood and spleen as compared with control group. The percentage of CD3+CD8+ T-cell were increased significantly in 1000mg/kg of GASSW groups only in spleen as compared with control groups. The CD4+/CD8+ T-cell ratios were increased significantly in 1000 and 500mg/kg of GASSW groups only in the blood as compared with control group. Goamshimshinhwan(GASSW) has immuno-stimulating effect on SD rats with deteriorated immunity caused by methotrexate.

Keywords

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