Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Cnidii Rhizoma on Phase II Enzyme and Ornithine Decarboxylase Activities

천궁이 Phase II 효소 유도와 Ornithine Decarboxylase 활성에 미치는 영향

  • Shon, Yun-Hee (Intractable Disease Research Center, Dongguk University) ;
  • Kim, Mee-Kyung (Intractable Disease Research Center, Dongguk University) ;
  • Cho, Hyun-Jung (Intractable Disease Research Center, Dongguk University) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine, Dongguk University)
  • 손윤희 (동국대학교 난치병한양방치료연구센터) ;
  • 김미경 (동국대학교 난치병한양방치료연구센터) ;
  • 조현정 (동국대학교 난치병한양방치료연구센터) ;
  • 남경수 (동국대학교 의과대학 약리학교실)
  • Published : 2006.12.25
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Abstract

Water extract from Cnidii Rhizoma (CRW) was tested for colon cancer chemopreventive activity by measuring the induction of phase II detoxification enzyme activity [quinone reductase (QR) and glutathione S-transferase (GST)] and glutathion (GSH) levels and ornithine decarboxylase (ODC) activity in cultured human colorectal adenocarcinoma HT-29 cells. CRW inhibited cell proliferation in cultured HT-29 cells. CRW induced QR activity in a dose-dependent manner in a concentration range of 0.1${\sim}$5.0 $mg/m{\ell}$. GST activity was also induced with the treatment of CRW in HT-29 cells. In addition GSH levels was increased with CRW. CRW inhibited ODC activity, a key enzyme of polyamine biosynthesis, which is enhanced in tumor promotion. These results suggest that CRW has colon cancer chemopreventive activity by increasing phase II enzyme activity and GSH levels and inhibiting ODC activity in vitro.

Keywords

References

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