Journal of Microbiology

  • 제44권2호
  • /
  • Pages.200-205
  • /
  • 2006
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Microarray-Mediated Transcriptome Analysis of the Tributyltin (TBT)-Resistant Bacterium Pseudomonas aeruginosa 25W in the Presence of TBT

  • Dubey Santosh K. (Center for Marine Environmental Studies (CMES), Ehime University, Department of Microbiology, Goa University) ;
  • Tokashiki Tsutomu (Center for Marine Environmental Studies (CMES), Ehime University) ;
  • Suzuki Satoru (Center for Marine Environmental Studies (CMES), Ehime University)
  • 발행 : 2006.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The tributyltin (TBT)-resistant bacterium, Pseudomonas aeruginosa 25W, which was isolated in seawater from the Arabian Sea, was subjected to transcriptome analysis in the presence of high concentrations of TBT. Only slight effects were observed at TBT concentration of $50{\mu}M$, but exposure to $500{\mu}M$ resulted in the upregulation of 6 genes and the downregulation of 75. Among the 75 downregulated genes, 53% (40 out of 75) were of hypothetical function, followed by 14 transcriptional regulation- and translation-associated genes. The results of this study indicated that although the 25W strain was highly resistant to TBT, high concentrations of TBT result in toxic effect on the transcriptional and translational levels. The target genes likely belong to a specific category of transcription- and translation-associated genes rather than to other gene categories.

키워드

참고문헌

  1. Fukagawa, T., S. Suzuki, K. Fukunaga, T. Suzuki, and K. Takama. 1992. Isolation and characterization of tributyltin chloride-resistant marine Vibrio. FEMS Microbiol. Lett. 93, 83-86 https://doi.org/10.1111/j.1574-6968.1992.tb05044.x
  2. Fukagawa, T. and S. Suzuki. 1993. Cloning of gene responsible for tributyltin chloride (TBTCl) resistance in TBTClresistant marine bacterium, Alteromonas sp. M-1. Biochem. Biophys. Res. Commun. 194, 733-740 https://doi.org/10.1006/bbrc.1993.1883
  3. Hoch, M. 2001. Organotin compounds in the environment, an overview. Appl. Geochem. 16, 719-743 https://doi.org/10.1016/S0883-2927(00)00067-6
  4. Jude, F., C. Arpin, C. Brachet-Castang, M. Cadepuy, P. Caumette, and C. Quentin. 2004. TbtABM, a multidrug efflux pump associated with tributyltin resistance in Pseudomonas stutzeri. FEMS Microbiol. Lett. 232, 7-14 https://doi.org/10.1016/S0378-1097(04)00012-6
  5. Kawai, S., Y. Kurokawa, H. Hirano, and M. Fukushima. 1998. Degradation of tributyltin by a bacterial strain isolated from polluted river water. Environ. Pollut. 102, 259-263 https://doi.org/10.1016/S0269-7491(98)00079-7
  6. Kimata, N., T. Nishino, S. Suzuki, and K. Kogure. 2004. Pseudomonas aeruginosa isolated from marine environments in Tokyo Bay. Microb. Ecol. 47, 41-47 https://doi.org/10.1007/s00248-003-1032-9
  7. Miller, J.H. 1972. Experiments in molecular genetics, p. 431-435. Cold Spring Harbor Laboratory Press, Plainview, N.Y
  8. Palma, M., S. Worgall, and L.E.N. Quadri. 2003. Transcriptome analysis of the Pseudomonas aeruginosa response to iron. Arch. Microbiol. 180, 374-379 https://doi.org/10.1007/s00203-003-0602-z
  9. Palma, M., D. DeLuca, S. Worgall, and L.E.N. Quadri. 2004. Transcriptome analysis of the response of Pseudomonas aeruginosa to hydrogen peroxide. J. Bacteriol. 186, 248-252 https://doi.org/10.1128/JB.186.1.248-252.2004
  10. Roy, U., S.K. Dubey, and S. Bhosle. 2004. Tributyltin chloride -utilizing bacteria from marine ecosystem of west coast of India. Curr. Sci. 86, 702-705
  11. Rudel, H., P. Lepper, and J. Steinhanses. 2003. Retrospective monitoring of organotin compounds in marine biota from 1985 to 1999: results from German environmental specimen bank. Environ. Sci. Technol. 37, 1731-1738 https://doi.org/10.1021/es026059i
  12. Stover, C.K., X.Q. Pham, A.L. Erwin, S.D. Mizoguchi, P. Warrener, M.J. Hickey, F.S. Brinkman, W.O. Hufgagle, D.J. Kowalik, M. Lagrou, R.L. Garber, L. Goltry, E. Tolentino, S. Westrock-Wadman, Y. Yuan, L.L. Brody, S.N. Coulter, K.R. Folger, A. Kas, K. Larbig, R. Lim, K. Smith, D. Spencer, G.K. Wong, Z. Wu, and I.T. Paulsen. 2000. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406, 959-964 https://doi.org/10.1038/35023079
  13. Suzuki, S., T. Fukagawa, and K. Takama. 1992. Occurrence of tributyltin-tolerant bacteria in tributyltin- or cadmiumcontaining seawater. Appl. Environ. Microbiol. 58, 3410-3412
  14. von Ballmoos, C., J. Brunner, and P. Dimroth. 2004. The ion channel of F-ATP synthase is the target of toxic organotin compounds. Proc. Natl. Acad. Sci. USA 101, 11239- 11244
  15. Wuertz, S., C.E. Miller, R.M. Pfister, and J.J. Cooney. 1991. Tributyltin-resistant bacteria from estuarine and freshwater sediments. Appl. Environ. Microbiol. 57, 2783-2789