한국환경성돌연변이발암원학회지 (Environmental Mutagens and Carcinogens)

한국환경성돌연변이발암원학회 (Korean Environmental Mutagen Society)
권호 표지

식용 달팽이 [Achatina fulica]로부터 항균성 물질의 분리 및 정제

Isolation and Purification of Antimicrobial Substance from the Giant Snail, Achatina fulica

  • 김인혜 (신라대학교 공과대학 제약공학과) ;
  • 현진원 (제주대학교 의과대학) ;
  • 이재화 (신라대학교 공과대학 제약공학과)
  • Kim In-Hae (Department of Pharmaceutical Engineering, College of Engineering, Silla University) ;
  • Hyun Jin-Won (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Lee Jae-Hwa (Department of Pharmaceutical Engineering, College of Engineering, Silla University)
  • 발행 : 2006.03.01
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초록

식용 달팽이 (Achatina fulica])의 추출물 RM 60을 사용하여 E. coli D31을 대상으로 순수한 항균성 물질을 분리 정제하였다. 정제한 항균성 물질은 MALDI-TOF Mass spectrometra를 사용하여 분자량을 측정한 결과, 1392.64 Da 단일 peak를 얻을 수 있었으며, 이 후 Edman 분해법을 이용한 peptide sequencer를 사용하여 일차구조 분석을 조사하고 있다.

An antimicrobial substance was purified from the giant snail body extract (Achatina fulica) using solid-phase extraction and separation on HPLC reversed-phase chromatography. The primary structure were determined by a combination of an automated amino acid sequence and MALDI-TOF Mass. Its molecular mass was found to be 1392.64 Da. This result was in excellent agrement with the theoretical molecular mass calculated from the amino acid sequence. purified peptide showed antimicrobial activity in vitro against Escherichia coli D31. This result indicate that giant snail whole body was potentially antimicrobial.

키워드

참고문헌

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