Effect of Limiting Factors for Hydrogen Production in Sulfur Deprived Chlamydomonas Reinhardtii

황결핍 된 Chlamydomonas Reinhardtii 배양액에서 수소생산을 위한 제한 인자들의 영향

  • Kim, Jun-Pyo (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Sim, Sang-Jun (Department of Chemical Engineering, Sungkyunkwan University)
  • 김준표 (성균관대학교 화학공학과) ;
  • 심상준 (성균관대학교 화학공학과)
  • Published : 2006.09.15

Abstract

Chlamydomonas reinhardtii is a green algae that can use light energy and water to produce hydrogen under anaerobic condition. This work reports the effect of limiting factors on hydrogen production in sulfur deprived anaerobic C. reinhardtii culture. In order to confirm the relationship between hydrogen production and limiting factors such as residual PSII activity and endogenic substrate degradation, the increase in chlorophyll concentration and the decrease in starch concentration was investigated during sulfur deprivation. The overall hydrogen production increased depending on cell density in range of $0.4{\sim}0.96\;g$ DCW/l. At this time, the increase in chlorophyll concentration during 24 h after sulfur deprivation increased in proportion to hydrogen production, however, the decrease in starch concentration was not proportional to that. Therefore, hydrogen production under sulfur deprivation using green alga was closely associated with the residual PSII activity than the endogenic substrate degradation.

Keywords

References

  1. S. Dunn, 'Hydrogen futures: toward a sustainable energy system', Int. J. Hydrogen Energy, Vol. 27, 2002, pp. 235-264 https://doi.org/10.1016/S0360-3199(01)00131-8
  2. I. Dincer, 'Technical and exergetic aspects of hydrogen energy systems', Int. J. Hydrogen Energy, Vol. 27, 2002, pp. 265-285 https://doi.org/10.1016/S0360-3199(01)00119-7
  3. D. Das and V. Nejat, 'Hydrogen production by biological processes: a survey of literature', Int. J. Hydrogen Energy Vol. 26, 2001, pp. 13-28 https://doi.org/10.1016/S0360-3199(00)00058-6
  4. M. L. Ghirardi, L. Zhang, J. W. Lee, T. Flynn, M. Seibert, E. Greenbaum, and A. Melis, 'Microalgae: a green source of renewable $H_{2}$.', Trends Biotechnol. Vol. 18, 2000, pp. 506-511 https://doi.org/10.1016/S0167-7799(00)01511-0
  5. A. Melis, L. Zhang, M. Forestier, M. L. Ghirardi, and M. Seibert, 'Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen eVolution in the green alga Chlamydomonas reinhardtii', Plant Physiol, Vol. 122, 2000, pp. 127-135 https://doi.org/10.1104/pp.122.1.127
  6. E. H. Harris, 'The Chlamydomonas sourcebook', Academic Press, Inc., San Diego, California, U.S.A., 1989, pp. 607-608
  7. A. Tsygankov, S. Kosourov, M. Seibert, M. L. Ghirardi, 'Hydrogen photoproduction under continuous illumination by sulfur-deprived, synchronous Chlamydomonas reinhardtii cultures', Int. J. Hydrogen Energy, Vol. 27, 2002, pp. 1239-1244 https://doi.org/10.1016/S0360-3199(02)00108-8
  8. J. P. Kim, C. D. Kang, S. J. Sim, M. S. Kim, T. H. Park, D. H. Lee, D. J. Kim, J. H. Kim, Y. K. Lee, D. Pak, 'Cell age optimization forhydrogen production induced by sulfur deprivation using a green alga CWamydomonas reinhardtii UTEX 90', J. Microbiol. Biotechnol., Vol. 15, 2005, pp. 131-135
  9. S. Kosourov, A. Tsygankov, M. Seibert, and M. L. Ghirardi, 'Sustained hydrogen photoproduction by CWamydomonas reinhardtii: Effects of Culture Parameters', Biotechnol. and Bioeng., Vol. 78, 2002, pp. 731-740 https://doi.org/10.1002/bit.10254
  10. T. K. Antal, T. E. Krendeleva, T. V. Laurinavichene, V. V. Makarova, M. L. Ghirardi, A. B. Rubin, A. A. Tsygankov, and M. Seibert, 'The dependence of algal $H_{2}$ production on PhotosystemIl and $O_{2}$ consumption activities in sulfur-deprived CWamydomonas reinhardtii cells', Biochimica et Biophysica Vol. 1607, 2003, pp. 153-160 https://doi.org/10.1016/j.bbabio.2003.09.008
  11. L. T. Zhang, Happe, and A. Melis, 'Biochemical and morphological characterization of suIfur-deprived and $H_{2}-Producing$ Chlamydomonas reinhardtii (green alga)', Planta, Vol. 214, 2002, pp. 552-561 https://doi.org/10.1007/s004250100660
  12. W. G. Hopkins and N. P. A. Huner, 'Introduction to plant physiology', 3rd ed., John Wiley & Sons, Inc., Hoboken, New Jersey, U.S.A. 2004, pp. 63-166