Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Occurrence of Rice black-streaked dwarf fijivirus in Maize

옥수수의 벼검은줄오갈병

  • Lee Bong-Choon (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Hong Yeon-Kyu (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Hong Sung-Jun (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Park Sung-Tae (Yeongnam Agricultural Research Institute, NICS, RDA)
  • 이봉춘 (농촌진흥청, 작물과학원, 영남농업연구소) ;
  • 홍연규 (농촌진흥청, 작물과학원, 영남농업연구소) ;
  • 홍성준 (농촌진흥청, 작물과학원, 영남농업연구소) ;
  • 박성태 (농촌진흥청, 작물과학원, 영남농업연구소)
  • Published : 2006.04.01
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Abstract

July in 2005, we collected infected maize plant that showing stripe dwarf disease on maize leaf in Jeonbuk provinces including Gochang-gun and conducted genomic dsRNA extraction and RT-PCR. Genomic dsRNA was extracted directly in infected maize plant and electrophoresis in agarose gel. We confirmed 10 segments of genomic dsRNA. We conducted RT-PCR by genomic dsRNA and specific primer of S7, S8 and S10. As a result, specific band of expected size was confirmed respectively. In the results of dsRNA and RT-PCR analysis, we confirmed Rice black-streaked dwarf fijivirus (RBSDV) from naturally infected maize plant. Occurrence of RBSDV of maize plant was dealt 22 ha's damage in maize field. The occurrence rate was 80% in a lot of places of disease.

2005년 7월 전북고창 지역에서 옥수수에 줄무늬 위축증상을 나타내는 이병주를 채집하여 게놈 dsRNA 분리 및 RT-PCR 반응을 실시하였다. 게놈 dsRNA 분리는 이병엽 100mg에서 직접 dsRNA를 추출하여 agarose gel에서 전기영동 하였으며, 10개의 분절을 확인하였다. 추출한 dsRNA를 주형으로 하여 S7, S8, S10 full-length 특이적인 primer를 제작하여 RT-PCR을 실시하였다. 그 결과 각각의 분절에서 예상되는 크기의 밴드를 확인하였다. 고창지역의 사료용 옥수수 재배지에서 RBSDV의 발생면적은 약 22ha에 달하였으며 발생이 심한 곳은 약 80%의 이병율을 나타내기도 하였다.

Keywords

References

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Cited by

  1. In Vitro Expression and Antibody Preparation of Rice black-streaked dwarf virus Coat Protein Gene vol.22, pp.1, 2016, https://doi.org/10.5423/RPD.2016.22.1.32
  2. Sequence Analysis of Segments 8 and 10 of Rice black-streaked dwarf virus from Maize Plants vol.18, pp.4, 2012, https://doi.org/10.5423/RPD.2012.18.4.387