Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Genetic Transformation of a Mushroom Forming Fungus Coprinellus congregatus to an Antibiotic Resistance Using Oidia Instead of Protoplast Generation

먹물버섯 Coprinellus congregatus에서 분열자를 사용한 형질전환

  • Park, Nam-Mee (Microbial Physiology Lab, Division of Life Sciences, Kangwon National University) ;
  • Kim, Dong-Sik (Microbial Physiology Lab, Division of Life Sciences, Kangwon National University) ;
  • Choi, Hyoung-T. (Microbial Physiology Lab, Division of Life Sciences, Kangwon National University)
  • 박남미 (강원대학교 생명과학부 미생물생리학 실험실) ;
  • 김동식 (강원대학교 생명과학부 미생물생리학 실험실) ;
  • 최형태 (강원대학교 생명과학부 미생물생리학 실험실)
  • Published : 2006.03.01
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Abstract

Genetic transformation of a mushroom-forming fungus Coprinellus congregatus to antibiotic resistance gene had been successfully carried out by electroporation to oidia instead of protoplasts. Since there was no protoplast generation step which required not only cell wall degrading enzymes but many skillful procedures, commercial herbicide (basta) could be used without any difficulty with simple procedure. The transformation yield was 10-20 $transformants/{\mu}g$ DNA, and the transformants were very stable even after 10 consecutive transfers through the non-selective medium.

먹물버섯의 하나인 Coprinellus congregatus를 대상으로 유전자의 도입을 위한 형질전환실험에서 원형질체를 생성하지 않고 분열자 (oidium)를 사용하는 방법을 확립하였다. 분열자는 20일 이상된 CKMM 한천배지에서 생성되며, 이를 밤샘배양으로 발아를 촉진시킨 상태에서 전기천공방법으로 항생물질 basta에 대한 형질전환을 수행한 결과 10-20 형질전환체/${\mu}g$ DNA의 수율로 형질전환체를 확보할 수 있었다. 이 형질전환체들은 도입된 벡터가 염색체 상에 삽입되어 유전적으로 안정된 상태를 유지하였다.

Keywords

References

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