Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of Dietary Vitamin E Levels on Carcass Characteristics, Vitamin E Concentration of Longissimus Muscle and Lipid Oxidation in Hanwoo Steers

사료중의 비타민 E 수준이 한우 거세우의 도체특성, 배최장근내 비타민 E 함량 및 지방산화에 미치는 영향

  • Published : 2006.12.31
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Abstract

This experiment was carried out to investigate the effects of dietary vitamin E levels on carcass characteristics and vitamin E contents, fatty acid composition and lipid oxidation as indicated by thiobarbituric acid reactive substances(TBARS) of the longissimus muscle in Hanwoo steers. Forty Hanwoo steers were randomly assigned to one of four groups and fed the diet containing 200(control), 1000, 2000 or 2500 IU a(alpha)-tocopherol acetate/head/day for 3 months of last finishing period. Backfat thickness was thinner and beef fat color was whiter(P<0.05) in steers fed the vitamin E supplemented diets than those fed the control diet. But the longissimus muscle area and yield grade did not differ among treatments. Marbling score and quality grade were higher(P<0.05) in steers fed the diets containing 1000 and 2000 IU vitamin E than those fed the other diets. Vitamin E concentration in the longissimus muscle was higher(P<0.05) as the vitamin E content in diet was higher. The levels of oleic and linoleic acids in the longissimus muscle were not affected by dietary vitamin E levels. But linolenic and arachidonic acids were lower in steers fed the vitamin E supplemented diets than those fed the control diet. TBARS in the longissimus muscle was not affected by the vitamin E levels in diet until 5th day of storage but delayed muscle lipid oxidation in steers fed the vitamin E supplemented diets after 7th day of storage. Therefore according to this result, it may be concluded that steers have a higher quality grade and higher vitamin E concentration in muscle and lower in TBARS when diet contains 1000 or 2000 IU vitamin E per kg of diet.

본 시험은 사료중의 비타민 E 수준이 한우 거세우의 도체특성과 쇠고기중의 비타민 E 함량 및 지방산화에 미치는 영향을 규명하기 위하여 실시하였다. 처리구는 하루 한 마리당 비타민 E를 200, 1,000, 2,000 및 2,500 IU를 급여하는 4개 구를 두고 비육후기 출하 전 90일 동안 시험사료를 급여하였고, 한 처리 당 한우 거세우를 10두씩 배치하였다. 비타민 E는 a(alpha)-tocopheryl acetate(Rovimix E-50 SD, Roche Vitamins Co., France)를 사용하였다. 사양시험 종료직후 도체등급판정을 받았고, 도체 배최장근 중의 비타민 E 함량, 지방산 조성 및 TBARS를 측정하였다.등지방은 비타민 E 급여구가 대조구 보다 얇았고, 지방색은 비타민 E 급여구가 대조구 보다 더 백색화 되었다. 배최장근 단면적과 육량등급은 처리구간에 차이가 없었다. 근내지방도와 육질등급은 비타민 E 1,000 IU와 2,000 IU 구가 다른 처리구보다 높았다. 도체 배최장근중의 비타민 E 함량은 비타민 E 급여수준이 높아질수록 높아졌다. 배최장근 중의 지방산 함량 중에서 oleic acid 과 linoleic acid 함량은 사료중의 비타민 E 수준에 의하여 영향을 받지 않았으나, linolenic acid와 arachidonic acid 함량은 비타민 E 급여구가 대조구보다 낮았다.지방산패도는 저장 5일째까지는 비타민 E 수준에 의하여 영향을 받지 않았으나 저장 7일째부터는 비타민 E 급여구가 대조구보다 지방의 산패도가 지연되었다. 그러므로 본 시험결과에 의하면 한우 거세우에게 하루 한 마리당 비타민 E를 1,000 또는 2,000 IU를 급여하는 것이 육질등급 및 도체중의 비타민 E 함량 높이고 도체의 지방산패도를 지연시키는데 도움이 되리라 사료된다.

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References

  1. Arnold, R. N., Scheller, K. K., Arp, S. C., Williams, S. N, Buege, D. R. and Schaefer, D. M. 1992. Effect of Long- or Short-Term Feeding of $\alpha$-Tocopheryl Acetate to Holstein and Crossbred Beef Steers on Performance, Carcass Characteristics, and Beef Color Stability. J. Anim. Sci. 70:3055- 3065 https://doi.org/10.2527/1992.70103055x
  2. Arnold, R. N., Arp, S. C., Scheller, K. K., Williams, S. N. and Schaefer, D. M. 1993. Tissue equilibration and subcellular distribution of vitamin E relative to myoglobin and lipid oxidation in displayed beef. J. Anim. Sci. 71:105-118 https://doi.org/10.2527/1993.711105x
  3. Buege, J. A. and Aust, S. D. 1978. Microsomal lipid peroxidation. Method in emzymol. 105:302- 303
  4. Chan, W. K. M., Hakkarainen, K., Faustman, C., Schaefer, D. M., Scheller, K. K. and Liu, Q. 1996. Dietary vitamin E effect on color stability and sensory assessment of spoilage on three beef muscles. Meat Sci. 42(4):387-399 https://doi.org/10.1016/0309-1740(95)00055-0
  5. Demeyer, D., Hoozee, J. and Meadom, H. 1974. Specificity of lipolysis during dry sausage ripening. J. Food Sci. 39:293-296 https://doi.org/10.1111/j.1365-2621.1974.tb02878.x
  6. Dryden, F. D. and Marchello, J. A. 1970. Influence of total lipid and fatty acid composition upon the palatability of three bovine muscle. J. Anim. Sci. 31:36-41 https://doi.org/10.2527/jas1970.31136x
  7. Enser, M., Hallett, K. G., Hewett, B., Fursey, G. A. J., Wood, J. D. and Harrington, G. 1998. Fatty acid content and composition of UK beef and lamb muscle in relation to production system and implications for human nutrition. Meat Science. 49(3):329-341 https://doi.org/10.1016/S0309-1740(97)00144-7
  8. Faustman, C., Cassens, R. G., Schaefers, D. M., Buege, D. R. and Scheller, K. K. 1989a. Vitamin E supplementation of Holstein steer diet improved sirloin steak color. J. Food Sci. 54:485-486 https://doi.org/10.1111/j.1365-2621.1989.tb03114.x
  9. Faustman, C., Cassens, R. G., Schaefers, D. M., Buege, D. R., Williams, S. N. and Scheller, K. K. 1989b. Improvement of pigment and lipid stability in Holstein steer beef by dietary supplementation with vitamin E. J. Food Sci. 54:858-862 https://doi.org/10.1111/j.1365-2621.1989.tb07899.x
  10. Folch, J., Lees, M. and Stanley, G. H. S. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509
  11. Gatellier, P., Hamelin, C., Durand, Y. and Renerre, M. 2001. Effect of a dietary vitamin E supplementation on colour stability and lipid oxidation of air- and modified atmosphere packaged beef. Meat Sci. 59:133-140 https://doi.org/10.1016/S0309-1740(01)00063-8
  12. Horstein, I., Crowe, P. F. and Hiner, R. 1967. Composition of lipids on some beef muscle. J. Food Sci. 32:650-659 https://doi.org/10.1111/j.1365-2621.1967.tb00856.x
  13. Lahucky, R., Novotna, K., Zaujec, K., Mojto, J., Pavlic, M. and Roa, N. E. B. 2002. Effects of dietary vitamin E supplementation on a-tocopherol content and antioxidative status of beef muscles. Czech J. Animal Scl. 47(9):381-386
  14. Lee, S. K., Kim, Y. S., Liang, C. Y. and Song, Y. H. 2003. Effects of dietary vitamin E supplementation on color stability, lipid oxidation and reducing ability of Hanwoo(Korean cattle) beef during retail display. Asian-Aust. J. Anim. Sci. 16(10):1529-1535 https://doi.org/10.5713/ajas.2003.1529
  15. Link, B. A., Bray, R. W., Cassens, R. G. and Kauffman, R, G. 1970. Fatty acid composition of bovine skeletal muscle lipids during growth. J. Anim. Sci. 30:726-731 https://doi.org/10.2527/jas1970.305726x
  16. Lynch, M. P., Kerry, J. P., Buckley, D. J., Faustman, C. and Morriessey, P. A. 1999. Effect of dietary vitamin E supplementation on the colour and lipid stability of fresh, frozen and vacuum packed beef. Meat Sci. 52(1):95-99 https://doi.org/10.1016/S0309-1740(98)00153-3
  17. Lynch, A., Kerry, J. P., O'Sullivan, M. G., Lawlor, J. B. P., Buckley, D. J. and Morriessey, P. A. 2000. Distribution of a-tocopherol in beef muscles following dietary a-tocopherol acetate supplementation. Meat Sci. 56(2):211-214 https://doi.org/10.1016/S0309-1740(00)00033-4
  18. Melton, S. L., Amiri, M., Davis, G. W. and Backus, W. R. 1982. Flavor and chemical characteristics of ground beef from grass-, forage-grain and grain- finished steers. J. Anim. Sci(55):77-87
  19. Mitsumoto, M., Ozawa, S., Mitsuhashi, T. and Koide, K. 1998. Effects of dietary vitamin E supplementation for one week before slaughter on drip, colour and lipid stability during display in Japaness black steer beef. Meat Sci. 49(2):165- 174 https://doi.org/10.1016/S0309-1740(97)00123-X
  20. National livestock and meat board. 1988. Nutrient values of muscle food: Composition values of specific cuts of meat, poultry and fish. Nationals livestock and meat board. Chicago. IL
  21. Oka, A., Iwaki, F., Dohgo, T., Ohtagaki, S., Noda, M., Shiozaki, T., Endoh, O. and Ozaki, M. 2002. Genetic effects on fatty acid composition of carcass fat of Japanese black wagyu steers. J. Anim. Sci. 80: 1005-1011
  22. Realini, C. E., Duckett, S. K., Brito, G. W., Rizza, M. D. and Mattos, D. D. 2003. Effect of pasture vs. concentrate feeding with or without antioxidants on carcass characteristics, fatty acid composition and quality of Uruguayan beef. Meat Sci. 66(3):567-577 https://doi.org/10.1016/S0309-1740(03)00160-8
  23. Rivera, J. D., Duff, G. C., Galyean, M. L., Walker, D. A. and Nunnery, G. A. 2002. Effects of supplemental vitamin E on performance, health, and humoral immune response of beef cattle. J. Anim. Sci. 80:933-941
  24. Rumsey, T. S., Oltjen, R. R., Bovard, K. P. and Priode, B. M. 1972. Influence of widely diverse finishing regimens and breeding on depot fat composition in beef cattle. J. Anim. Sci. 35:1069- 1075 https://doi.org/10.2527/jas1972.3551069x
  25. SAS. 2000. SAS/STAT user's guide. Version 8, SAS Institute Inc., Cary, N. C., USA
  26. Schwarz, F. J., Augustini, C., Timm, M. and Kirchgener, M. 1998a. Improvement of the quality of beef by dietary vitamin E supplementation in the finishing period of young bulls: I. Experimental design, carcass quality and meat quality. Fleischwirtschaft. 78:134-137
  27. Schwarz, F. J., Augustini, C., Timm, M., Kirchgener, M. and Steinhart, H. 1998b. Effect of vitamin E on $\alpha$-tocopherol concentration in different tissues and oxidative stability of bull beef. Livestock Production Sci. 56:165-171 https://doi.org/10.1016/S0301-6226(98)00189-4
  28. Secrist, D. S., Hill, W. J., Owens, F. N., Koevering, M. T., Strasia, C. A., Dolezal, H., G., Gardner, B. A., Gill, D. R. and Van Koevering, M. T. 1995. Animal Science Research Report Agricultural Experiment Station, Oklahoma State University. No. 943, 137-141
  29. Waldman, R. C., Suess, G. G. and Brungardt, V. H. 1968. Fatty acids of certain bovine tissue and their association with growth, carcass and palatability traits. J. Anim. Sci. 27:632-635 https://doi.org/10.2527/jas1968.273632x
  30. Westerling, D. B. and Hedrick, H. B. 1979. Fatty acid composition of bovine lipids as influenced by diet, sex and anatomical location and relationship to sensory characteristics. J. Anim. Sci. 48:1343- 1348 https://doi.org/10.2527/jas1979.4861343x
  31. Witte, V. C., Krause, G. F. and Baile, M. E. 1970. A new extraction method for determining 2-thiobarbituric acid values of pock and beef during storage. J. Food Sci. 35:582-585 https://doi.org/10.1111/j.1365-2621.1970.tb04815.x
  32. Yang, A., Larsen, T. W., Powell, V. H. and Tume, R. K. 1999. A comparison of fat composition of Japanese and long-term grain-fed Australian steers. Meat Sci. 51:1-9 https://doi.org/10.1016/S0309-1740(98)00065-5
  33. Yoshimura T. and Namikawa, K. 1983. Influence of breed, sex and anatomical location on lipid and fatty acid composition of bovine subcutaneous fat. Jpn. J. Zootech. Sci. 54:97-105
  34. 山內 淸. 1987. 肉. 肉製品の脂質酸化と warmed- over flavor. 食肉の科學. 28:165
  35. 生化學辭典. 1984. 第1版. 東京化學同人. 東京
  36. 김수민, 이신호, 성삼경. 1997. Vitamin C와 Vitamin E 처리가 한우육의 육색 및 지방산화에 미치는 영향. 한국축산학회지. 39(3):267-274
  37. 김용선, 양성운, 김주용, 박연수, 황환섭, 이성기. 2002. Vitamin E와 selenium의 급여가 비거세우육의 진열저장 중 육색 안정성에 미치는 영향. 한국축산식품학회지. 22(2):108-114
  38. 농림부. 2005. 축산물 생산비 통계자료. 번식우 및 비육우
  39. 문성실, 주선태, 이정일, 박구부. 2001. 근내지방도와 성숙도가 저장기간에 따른 한우육의 이화학적 특성 변화에 미치는 영향. 한국축산식품학회지. 21(1):47-55
  40. 박구부, 이재숙, 이한기, 송또준. 1989. 저장기간에 따른 한우육 및 돈육의 지방산 조성변화. 한국축산학회지. 31:254
  41. 이성수, 정재경, 박노형, 원유석. 1997. 거세가 한우의 도체특성과 혈청 대사물질에 미치는 영향. 한국축산학회지. 39(2):145-154
  42. 추교문, 조희웅, 안병홍. 2004. 비타민 C 및 E 급여가 한우 거세우 육의 지방산화 및 육색안정성에 미치는 영향. 동물자원지 46(4):635-644
  43. 축산물등급판정소. 2005. 축산물등급판정연보
  44. 최양일, 김영규, 이창림. 1995. 포장방법 및 숙성 온도가 한우육의 육색, 연도 및 저장특성에 미치는 영향. 한국축산학회지. 37(6):639-650

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