Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Thermostable Xylanase Encoded by xynA of Streptomyces thermocyaneoviolaceus: Cloning, Purification, Characterization and Production of Xylooligosaccharides

  • CHOI JUN-HO (Department of Agricultural Chemistry and Institute of Agricultural Science & Technology, Kyungpook National University) ;
  • LEE OH-SEUK (Department of Agricultural Chemistry and Institute of Agricultural Science & Technology, Kyungpook National University) ;
  • SHIN JAE-HO (Department of Agricultural Chemistry and Institute of Agricultural Science & Technology, Kyungpook National University) ;
  • KWAK YUN-YOUNG (Department of Agricultural Chemistry and Institute of Agricultural Science & Technology, Kyungpook National University) ;
  • KIM YOUNG-MOG (Department of Agricultural Chemistry and Institute of Agricultural Science & Technology, Kyungpook National University) ;
  • RHEE IN-KOO (Department of Agricultural Chemistry and Institute of Agricultural Science & Technology, Kyungpook National University)
  • Published : 2006.01.01
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Abstract

We have cloned a xylanase gene (xynA) from Streptomyces thermocyaneoviolaceus. The deduced amino acid sequences of the XynA, including the active site sequences of glycosyl hydrolase family 10, showed high sequence homology with several xylanases assigned in this category. The XynA was overexpressed under an IPTG inducible T7 promoter control in E. coli BLR(DE3). The overproduced enzymes were excreted into culture supernatants and periplasmic space. The purified XynA had an apparent molecular mass of near 54 kDa, which corresponds to the molecular mass calculated from its gene. The optimum pH and temperature of the purified XynA were determined to be 5.0 and $65^{\circ}C$, respectively. The XynA retained over $90\%$ its activity after the heat treatment at $65^{\circ}C$ for 30 min. The XynA was highly efficient in producing xylose (X1), xylobiose (X2), xylotriose (X3), and xylotetraose (X4) from xylan.

Keywords

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