Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Effect of Ponciretin on Helicobacter pylori VacA Toxin-induced Vacuolation in HeLa Cells

  • KIM JONG-MI (College of Pharmacy, Kyung Hee University) ;
  • SHIN JI-EUN (Department of Food and Nutrition, Kyung Hee University) ;
  • BAE EUN-AH (Department of Food and Nutrition, Kyung Hee University) ;
  • HAN MYUNG JOO (Department of Food and Nutrition, Kyung Hee University) ;
  • KIM DONG-HYUN (College of Pharmacy, Kyung Hee University)
  • Published : 2006.01.01
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Abstract

The inhibitory effects of flavanone derivatives on Helicobacter pylori (HP) growth, infection and VacA toxininduced vacuolation were investigated. Among flavanones tested, ponciretin potently inhibited the growth of HP with a MIC value of 0.01 mg/ml and VacA toxin-induced vacuolation in HeLa cells with $IC_{50}$ value of 0.078 mM. However, other flavanones inhibited neither HP growth nor VacA toxininduced vacuolation. All flavanones tested did not inhibit HP infection to KATO III cells. Ponciretin also inhibited activation of procaspase-3 to caspase-3 in HeLa cell induced by HP VacA toxin, but did not affect Bax and Bcl-2 protein levels. These findings indicate that ponciretin inhibits growth as well as vacuolation by HP VacA toxin, which induces cell death via proteolytic activation of a cascade of caspases.

Keywords

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