Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Electrochemical properties of the mugwort-embedded biosensor for the determination of hydrogen peroxide

쑥을 이용한 과산화수소 정량 바이오센서의 전기화학적 성질

  • Lee, Beom-Gyu (Department of Chemistry, Chosun University) ;
  • Park, Sung-Woo (Department of Scientific Criminal Investigation, Graduate School of Peace and Security Studies, Chungnam National University) ;
  • Yoon, Kil-Joong (Division of Applied Sciences, Cheongju University)
  • 이범규 (조선대학교 자연과학대학 화학과) ;
  • 박성우 (충남대학교 평화안보대학원 과학수사과) ;
  • 윤길중 (청주대학교 이공대학 응용과학부)
  • Received : 2005.11.01
  • Accepted : 2006.01.10
  • Published : 2006.02.27
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Abstract

A mugwort-tissue-based modified carbon paste electrode was constructed for the amperometric detection of hydrogen peroxide and its electrochemical properties are described. Especially the amperometric signal was very stable and bigger than any other enzyme electrode studied in this lab. The effect of tissue composition on the response was linear within the wide range of experiment and the linearity of Lineweaver-Burk plot showed that the sensing process of the biosensor is by enzymatic catalysis. And pH dependent current profile connoted that two isozymes are active in this system.

탄소반죽에 쑥조직을 혼입시켜 과산화수소 정량 바이오센서를 제작하고 그것의 전기화학적 성질을 조사하였다. 다른 생체조직을 이용하여 제작한 센서와 비교할 때, 생촉매 안정성이 뛰어났으며, 보다 큰 감응신호를 보여주었다. 조직의 함량 변화에 따른 신호의 변화는 넓은 범위에 걸쳐 직선성을 보여 주었고, Lineweaver-Burk 도시의 직선성은 전극 감응이 효소 촉매작용에 의하여 조절되고 있음을 보였다. 또 감응전류의 pH 의존성 변화는 센서가 두 종류의 동위효소를 포함하고 있음을 암시하였다.

Keywords

References

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