Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Acetylcholinesterase Inhibition and Behavioral Changes of Crucian carp (Carassius auratus) Exposed to the Waterborne Parathion

Parathion에 노출된 붕어(Carassius auratus)의 Acetylcholinesterase 억제와 행동변화

  • Cho, Kyu-Seok (Department of Inland Fisheries Research Institute, Chung Cheong Buk-Do) ;
  • Park, Jong-Ho (Department of Environmental Engineering, Chung Ju National University) ;
  • Lee, Won-Ho (Department of Construction & Urban Engineering, Chung Ju National University) ;
  • Kang, Ju-Chan (Department or Aquatic Lire Medicine, Pukyong National University)
  • 조규석 (충청북도내수면연구소) ;
  • 박종호 (충주대학교 환경공학과) ;
  • 이원호 (충주대학교 건설도시공학과) ;
  • 강주찬 (부경대학교 수산생명의학과)
  • Received : 2006.01.09
  • Accepted : 2006.02.07
  • Published : 2006.03.30
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Abstract

The investigation of the Acetylcholinesterase (AChE) activity in tissues (brain, eye, muscle and serum) of crucian carp (Carassius auratus) exposed to the waterborne parathion was carried out for application as biomarker of organophosphate pesticides. The AChE activities were significantly inhibited in the experimental organs of C. auratus treated ${\geq}63{\mu}g/L$ of concentrations of parathion. The AChE activity of C. auratus was significantly reduced in response to brain (79.1~92.4%), eye (76.0~91.5%), muscle (89.7~97.6%) and serum (68.9~78.0%) after 30 days exposure. No significant mortality occurred during the experiment duration but behavioral changes occurred in the carp after exposure to the parathion were erratic swimming and convulsions. The anomaly in the carp exposed to parathion were observed in the form of scoliosis. The use of AChE activity and other adverse responses of the carp might be use as a reliable monitoring tool to detect parathion in aquatic ecosystem which might produce significant population changes.

파라치온에 노출된 붕어의 뇌, 안구, 근육 및 혈청의 AChE 활성 변화를 조사한 결과 파라치온 $63{\mu}g/L$ 농도 이상에서 유의하게 감소하는 경향을 보였다. 실험종료 시 AChE 활성은 뇌에서 79.1~92.4%, 안구에서 76.0~91.5%, 근육에서 89.7~97.6% 및 혈청에서 68.9~78.0% 감소하였다. 실험기간동안 유의한 생존율 변화는 관찰되지 않았으나, 유영이상 및 경련을 보였다. 또한 파라치온에 노출된 붕어의 형태변화는 척추측만(scoliosis)이 관찰되었는데 이러한 변화는 파라치온 $190{\mu}g/L$ 농도는 15%, $380{\mu}g/L$에서는 75%로 관찰되었다. 따라서 붕어의 AChE 활성과 그 밖의 행동 및 형태적 특성은 자연수계에서 파라치온 오염을 추정할 수 있는 생물학적 모니터닝 도구로 활용할 수 있다.

Keywords

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