Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Evolutionary Relationship of Liobagrus mediadiposalis (Teleostei: Amblycipitidae) Populations in Korea Inferred from Cytochrome b DNA Sequences

한국 고유종인 자가사리(Liobagrus mediadiposalis) 지역개체군의 분자진화적 유연관계

  • Kim, Maeng Jin (Faculty of Applied Marine Sciences, Cheju National University) ;
  • Han, Song-Hun (Faculty of Applied Marine Sciences, Cheju National University) ;
  • Yang, Hye-Young (Faculty of Applied Marine Sciences, Cheju National University) ;
  • Jo, Mi-Ran (Faculty of Applied Marine Sciences, Cheju National University) ;
  • Chung, Sang-Chul (Faculty of Applied Marine Sciences, Cheju National University) ;
  • Song, Choon Bok (Faculty of Applied Marine Sciences, Cheju National University)
  • Received : 2006.11.14
  • Accepted : 2006.11.28
  • Published : 2006.12.31
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Abstract

Phylogenetic relationships and DNA polymorphism among local populations of the Korean native catfish species, Liobagrus mediadiposalis, have been investigated based on mitochondrial cytochrome b DNA sequences. As a result, three genetically distinct groups of local populations were recognized based on the phylogenetic tree constructed. The first group was called "Nakdong-river group" that included the local populations from Geumho-river, Gyeongho-river, and Deokcheon-river; the second one was "Geum-river group"; the third one was represented as "Seomjin-river group" that included the samples from Seomjin-river, Dongjin-river and Geokum-do. The phylogeny also implied that the ancestral group of L. mediadiposalis have first evolved to Nakdong-river group, and later two local populations (Geum-river and Seomjinriver group) were diverged from the other lineage. DNA polymorphisms we observed were 4.4~4.7% between Geum-river group and Seomjin-river group, 5.1~5.5% between Seomjinriver group and Nakdong-river group, and 5.5~5.7% between Nakdong-river group and Geumriver group. These results indicated the long period of geographic isolation due to the river system in Korea caused such high degrees of DNA polymorphisms between local populations of L. mediadiposalis.

자가사리 지역개체군의 분자계통 진화적 유연관계를 알아보기 위하여 미토콘드리아 cytochrome b 유전자 서열을 이용하여 분석하였다. 그 결과, 우리나라 자가사리 개체군은 크게 3개의 집단으로 구분되었다. 즉 조상형 자가사리 개체군에서 먼저 낙동강 집단(금호강, 덕천강, 경호강 개체군)이 분화되었으며 그 후 섬진강 집단(동진강, 섬진강, 영산강, 거금도 개체군)과 금강 집단으로 분화되었을 것으로 추정할 수 있었다. 그리고 자가사리 개체군 집단 사이의 염기서열 차이는 금강집단과 섬진강집단 사이의 50~53 bp (4.4~4.7%)에 비해서 금강 집단과 낙동강 집단 사이의 차이는 63~65 bp (5.5~5.7%), 그리고 낙동강 집단과 섬진강 집단 사이에서 58~63 bp (5.1~5.5%)를 나타내어서 금강 집단과 낙동강 집단 사이에서 가장 유전적인 차이가 높은 것으로 나타났다. 이러한 개체군 집단의 분화양상과 유전적인 다형은 수계 형성과 같은 지리적 변화 때문에 생기는 오랫동안의 유전적 격리에 의한 것으로 분화시기 추정결과 이들 개체군 집단들의 격리 시기는 적어도 빙하기 이전으로 거슬러 올라가는 것으로 생각된다.

Keywords

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