Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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A Study on the Extractives of Domestic Major Softwood Needles(I) - Antioxidant Activity of the Extractives from the Needles of Abies koreana Maximowicz and Abies holophylla Wilson -

국내산 주요 침엽수 잎의 추출성분(I) - 구상나무(Abies koreana Maximowicz)와 전나무(Abies holophylla Wilson) 잎 추출성분의 항산화 활성 -

  • Lee, Sang-Keug (Korea Forest Research Institute) ;
  • Choi, Don-Ha (Korea Forest Research Institute) ;
  • Bae, Young-Soo (Dept. of Wood Science & Engineering, College of Forest Sciences, Kangwon National University)
  • Received : 2006.03.03
  • Accepted : 2006.03.24
  • Published : 2006.05.25
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Abstract

The dried needles (1.5 kg) of Abies koreana and Abies holophylla were ground, extracted with acetone-$H_2O$ (7:3, v/v), concentrated, and fractionated with a series of hexane, methylene chloride, ethyl acetate and water on a separatory funnel. Each fraction was freeze dried, then a portion of ethyl acetate soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluents. The isolated compounds were identified by cellulose TLC, $^1H$, $^{13}C-NMR$, COSY, HETCOR, FAB and EI-MS. The needles of Abies koreana and Abies holophylla contained a large amount of aromadendrin-7-O-${\beta}$-D-glucopyranoside (compound III), polydatin (compound VI), (-)-rhododendrol-2-O-${\beta}$-D-glucopyranoside (compound VII), in addition to a small amount of (+)-catechin (compound I), kaempferol-3-O-${\beta}$-D-glucopyranoside (compound IV), myricetin-3-O-${\beta}$-D-glucopyranoside (compound V), naringenin-7-O-${\beta}$-D-glucopyranoside (compound II). DPPH analysis was also tested to investigate the antioxidative effects on the isolated compounds and (+)-catechin and polydatin were effective.

구상나무(Abies koreana Maximowicz)와 전나무(Abies holophylla Wilson) 잎을 채취하여 건조시킨 후 분말로 제조하여 각각 1.5 kg을 아세톤-물(7:3, v/v)로 추출하고 헥산, 메틸렌클로라이드, 에틸아세테이트 및 수용성으로 분획하여 동결건조 시켰다. 에틸아세테이트용성 분획을 Sephadex LH-20으로 충진한 칼럼에서 메탄올과 에탄올-헥산 혼합액을 용리용매로 사용하여 칼럼크로마토그래피를 실시하였다. 단리된 화합물들은 TLC로 확인한 후 $^1H-$, $^{13}C-NMR$, COSY, HETCOR 등의 스펙트럼을 사용하여 정확한 구조를 규명하였고 FAB 및 EI-MS로써 분자량을 측정하였다. 많은 양의 aromadendrin-7-O-${\beta}$-D-glucopyranoside (화합물 III), polydatin (화합물 VI), (-)-rhododendrol-2-O-${\beta}$-D-glucopyranoside (화합물 VII)가 단리되었으며, 소량의 (+)-catechin (화합물 I), kaempferol-3-O-${\beta}$-D-glucopyranoside (화합물IV), myricetin-3-O-${\beta}$-D-glucopyranoside (화합물 V), naringenin-7-O-${\beta}$-D-glucopyranoside (화합물 II)도 단리 되었다. DPPH 라디칼 소거법을 이용하여 단리된 화합물들에 대한 항산화 활성시험을 실시하였으며 (+)- catechin과 polydatin이 항산화 효능을 나타내었다.

Keywords

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