Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
권호 표지

Retrospective Cohort Study on Genetic Susceptibility of Lead Exposure using Stored Blood in Specimen Bank-Focused Newly employed workers

시료보관은행에 저장된 혈액을 이용한 납 노출의 유전적 감수성에 관한 후향성 코호트 조사 연구 - 입사 1년차 대상자 연구

  • Kim, Nam-Soo (Institute of Industrial Medicine, Soonchunhyang University) ;
  • Kim, Jin-Ho (Institute of Industrial Medicine, Soonchunhyang University) ;
  • Lee, Kap-Soo (Institute of Industrial Medicine, Soonchunhyang University) ;
  • Lee, Sung-Soo (Institute of Industrial Medicine, Soonchunhyang University) ;
  • Kim, Hwa-Sung (Institute of Industrial Medicine, Soonchunhyang University) ;
  • Ahn, Kyu-Dong (Institute of Industrial Medicine, Soonchunhyang University) ;
  • Schwartz, Brian S. (Department of Epidemiology, Johns Hopkins School of Hygiene and Public Health) ;
  • Lee, Byung-Kook (Institute of Industrial Medicine, Soonchunhyang University)
  • 김남수 (순천향대학교 산업의학연구소) ;
  • 김진호 (순천향대학교 산업의학연구소) ;
  • 리갑수 (순천향대학교 산업의학연구소) ;
  • 이성수 (순천향대학교 산업의학연구소) ;
  • 김화성 (순천향대학교 산업의학연구소) ;
  • 안규동 (순천향대학교 산업의학연구소) ;
  • ;
  • 이병국 (순천향대학교 산업의학연구소)
  • Received : 2006.03.30
  • Accepted : 2006.05.23
  • Published : 2006.06.30
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Abstract

Genotype of ALAD and VDR yields two alleles, respectively and it has been implicated in susceptibility to lead toxicity. Also genotype known to variety by race. To evaluate the genetic susceptibility of ALAD and VDR gene on health effect of lead exposure, this study was done with new workers who entered lead industries from 1992 to 2001. Among database of lead industries of Soonchunhyang University Institute of Industrial Medicine, only new workers were selected for this study. The total of eligible workers for this category was 3,540 workers including non lead exposed workers of same lead industries. Genotype of ALAD and VDR were measured from stored blood in specimen bank of Soonchunhyang University, blood lead and other relevant information were obtained from database of each workers which were gathered at their first year of employment. Among 3,540 new employed study subjects during period of 1992-2001, 3204 workers(90.5%) had ALAD genotype 1-1; whereas 336 workers(9.5%) had variant type of ALAD (1-2 or 2-2). Lead exposed workers, 9.8%(n=243) male and 8.1%(n=16) female were heterozygous for the ALAD allele. Also non lead exposed workers, 8.9%(n=67) male and 9.3%(n=10) female were heterozygous for the ALAD allele. For VDR genotype, 2,903 workers(89.7%) out of total tested 3,238 workers were belonged to type bb and 335 workers(10.3%) were type bB or BB. Lead exposed workers, 10.4%(n=235) male and 12.2%(n=24) female were heterozygous for the VDR allele. Also non lead exposed workers, 9.2%(n=64) male and 12.5%(n=12) female were heterozygous for the VDR allele. No significant differences were seen in mean blood lead levels by ALAD and VDR genotype, nor was significantly associated with blood lead except age in multiple regression analysis.

Keywords

Acknowledgement

Supported by : 한국학술진흥재단

References

  1. Alessio L, Apostoli P, Crippa M. Influence of individual factors and personal habits on the levels of biological indicators of exposure. Toxicology letters 1995; 77: 93-103 https://doi.org/10.1016/0378-4274(95)03277-0
  2. Alexander BH, Checkoway H, Costa-Mallen P, Faustman EM, Woods JS, et al. Interaction of blood lead and aminolevulinic acid dehydratase genotype on markers of heme synthesis and ${\delta}$ sperm production in lead smelter workers. Environ Heal Persp 1998; 106(4): 213-216 https://doi.org/10.2307/3433966
  3. Astrin KH, Bishop DF, Wetmur JG, Kaul B, Davidow B, et al. ${\delta}$-aminolevulinic acid dehydratase isozymes and lead toxicity. Annals New York Academy of Sciences 1987; 5(4): 23-29
  4. Bell NH, Morrison NA, Nguyen TV, Eisman J, Hollis BW. Apa I polymorphisms of the vitamin D receptor predict bone density of the lumbar spine and not racial difference in bone density in young men. J Lab Clin Med 2001; 137(2): 133-140 https://doi.org/10.1067/mlc.2001.112095
  5. Benkmann HG, Bogdanski P, Goedde HW. Polymorphism of delta aminolevulinic acid dehydratase in various populations. Hum Hered 1983; 33: 62-64 https://doi.org/10.1159/000153351
  6. Bergdahl IS, Gerhardsson L, Schutz A, Desnick RJ, Wetmur JG, et al. Delta-aminolevulinic acid dehydratase polymorphism: Influence on lead levels and kidney function in humans. Arch Environ Health 1997; 52: 91-96 https://doi.org/10.1080/00039899709602870
  7. Chuang HY, Yu KT, Ho SK, Wu MT, Lin GT, et al. Investigations of vitamin D receptor polymorphism affecting worker' susceptibility to lead. J Occup Health 2004; 46: 316-321 https://doi.org/10.1539/joh.46.316
  8. Femandes FJ. Micromethod for lead determination in whole blood by atomic absorption with use of graphite furnace. Clin Chem 1975; 21: 555-561
  9. Fountas L, Moutsatsou P, Kastanias I, Tamouridis N, Tzanela M, et al. The contribution of vitamin D receptor gene polymorphisms in osteoporosis and familial osteoporosis. Osteoporos Int 1999; 10: 392-398 https://doi.org/10.1007/s001980050245
  10. Huang J, Ushiyama T, Inoue K, Kawasaki T, Hukuda S. Vitamin D receptor gene polymorphisms and osteoarthritis of the hand, hip and knee; a case-control study in Japan. Rheumatology 2000; 39: 79-84 https://doi.org/10.1093/rheumatology/39.1.79
  11. Kim HS, Lee SS, Lee GS, Hwangbo Y, Ahn KD, et al. The protective effect of ${\delta}$ aminolevulinic acid dehydratase 1-2 and 2-2 isozymes against blood lead with higher hematologic parameters. Environ Heal Persp 2004; 112(5): 538-541
  12. Onalaja AO, Claudio L. Genetic susceptibility to lead poisoning. Envrion Health Perspect 2000; 108(supplement 1): 23-28
  13. palomba S, Giuliano Numis F, Mossetti G, Rendina D, Vuotto P, et al. Raloxifene administration in postmenopausal women with osteoporosis: effect of different Bsm I vitamin D receptor genotypes. Human Reproduction 2003; 18(1): 192-198 https://doi.org/10.1093/humrep/deg031
  14. Schwartz BS, Lee BK, Stewart W, Ahn KD, Springer K, et al. Associations of ${\delta}$-aminolevulinic acid dehydratase genotype with plant, exposure duration, and blood lead and zinc protoporphyrin levels in Korean lead workers. Am J Epidemiol 1995; 142: 738-745 https://doi.org/10.1093/oxfordjournals.aje.a117705
  15. Schwartz BS, Lee BK, Lee GS, Steward WF, Simon D, et al. Associations of blood lead, dipercaptosuccinic acid-chelatable lead, and tibia lead with polymorphisms in the Vitamin D Receptor and d-Aminolevulinic Acid Dehydratase Genes. Environ Health Persp 2000: 108: 949-954 https://doi.org/10.2307/3435053
  16. Smith CM, Wang X, Hu H, Kelsey KT. A polymorphism in the ${\delta}$-aminolevulinic acid dehydratase gene may modify the pharmakokinetics and toxicity of lead. Environ Health Persp 1995; 103: 248-253 https://doi.org/10.2307/3432544
  17. Sousa M, Silva M, Duarte A, Azevedo E. Delta-aminolevulinate dehydrase (ALAD) polymorphism in mixed Brazilian from the State of Bahia. Gene Geography 1991; 5: 33-38
  18. Weaver VM, Schwartz BS, Ahn KD, Stewart WF, Kelsey KT, et al. Associations of renal function with polymorphi 는 in the ${\delta}$- aminolevulinic acid dehydratase, vitamin D receptor, and nitric ocide symthase genes in korean lead workers. Environ Health Persp 2003; 111(13): 1613-1619 https://doi.org/10.1289/ehp.6116
  19. Wetmur JG, Kaya AH, Plewinska M, Desnick RJ. Molecular characterization of the human ${\delta}$aminolevulinate dehydratase 2 (ALAD2) alle; Implications for molecular screening of individuals for genetic susceptibility to lead poisoning. Am J Hum Genet 1991; 49: 757-763
  20. Wetmur JG, Lehnert, Desnick RJ. The ${\delta}$aminolevulinate dehydratase polymorphism: higher blood lead levels in lead workers and environmentally exposed children with the 1-2 and 2-2 isozymes. Environmental Research 1991; 56: 109-119 https://doi.org/10.1016/S0013-9351(05)80104-5
  21. Wetmur JG. Influence of the common human ${\delta}$-aminolevulinate dehydratase polymorphism on lead body burden. Environ Health Perspect 1994; 102(suppl 3): 215-219 https://doi.org/10.1289/ehp.94102s5215
  22. Zajickova K, Zofkova I, Bahbouh R, Krepeldva A. Vitamin D receptor gene polymerphi는, bone mineral density and bone turnover: Fok genotype is related to postmenopausal bone mass. Physiol Res 2002; 51: 501-509