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Molecular Cloning and Characterization of a Novel Calcium-dependent Protein Kinase Gene IiCPK2 Responsive to Polyploidy from Tetraploid Isatis indigotica

  • Lu, Beibei (Department of pharmacognosy, School of Pharmacy, Second Military Medical University) ;
  • Ding, Ruxian (Department of pharmacognosy, School of Pharmacy, Second Military Medical University) ;
  • Zhang, Lei (Department of pharmacognosy, School of Pharmacy, Second Military Medical University) ;
  • Yu, Xiaojing (Department of Pharmacy, Changzheng Hospital, Second Military Medical University) ;
  • Huang, Beibei (Department of Pharmacy, Changzheng Hospital, Second Military Medical University) ;
  • Chen, Wansheng (Department of Pharmacy, Changzheng Hospital, Second Military Medical University)
  • Received : 2006.03.29
  • Accepted : 2006.06.07
  • Published : 2006.09.30
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Abstract

A novel calcium-dependent protein kinase gene (designated as IiCPK2) was cloned from tetraploid Isatis indigotica. The full-length cDNA of IiCPK2 was 2585 bp long with an open reading frame (ORF) of 1878 bp encoding a polypeptide of 625 amino acid residues. The predicted IiCPK2 polypeptide included three domains: a kinase domain, a junction domain (or autoinhibitory region), and a C-terminal calmodulin-like domain (or calcium-binding domain), which presented a typical structure of plant CDPKs. Further analysis of IiCPK2 genomic DNA revealed that it contained 7 exons, 6 introns and the length of most exons was highly conserved. Semi-quantitative RT-PCR revealed that the expression of IiCPK2 in root, stem and leaf were much higher in tetraploid sample than that in diploid progenitor. Further expression analysis revealed that gibberellin ($GA_3$), NaCl and cold treatments could up-regulate the IiCPK2 transcription. All our findings suggest that IiCPK2 might participate in the cold, high salinity and GA3 responsive pathways.

Keywords

References

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