Biotechnology and Bioprocess Engineering:BBE

  • 제11권6호
  • /
  • Pages.516-521
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  • 2006
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Characterization of gltA::luxCDABE Fusion in Escherichia coli as a Toxicity Biosensor

  • Ahn, Joo-Myung (Graduate School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Byoung-Chan (Advanced Environmental Monitoring Research Center, Gwangju Institute of Science and Technology (GIST)) ;
  • Gu, Man-Bock (Graduate School of Life Sciences and Biotechnology, Korea University)
  • 발행 : 2006.12.31
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초록

The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, EBJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens IuxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.

키워드

참고문헌

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