Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Performance of Anion Exchange Expanded Bed Adsorption Chromatography on the Recovery of G6PDH from Unclarified Feedstock with High Biomass Concentration

  • Chow, Yen Mei (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Tey, Beng Ti (Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Ibrahim, Mohd Nordin (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia) ;
  • Ariff, Arbakariya (Department of Bioprocess Technology, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia) ;
  • Ling, Tau Chuan (Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia)
  • Published : 2006.10.30
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Abstract

The bed stability of Streamline DEAE (p = 1.2 g/mL) in a 20mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).

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References

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